Diphosphatidyl Glycerol in Mycoplasma laidlawii

Abstract

The phospholipids of Mllycoplasina laidlawii strain B consist of phosphatidyl glucose and an unidentified compound. This unidentified phospholipid (fraction H) was shown to be neither a nitrogen-containing compound, a plasmalogen,

Effect of acyl chain composition on salt-induced lamellar to inverted hexagonal phase transitions in cardiolipin

“…Incipient non-lamellar phase-forming cardiolipins may additionally possess dynamic properties which can enhance the activity of integral membrane enzymes such as cytochrome oxidase [13], as found also for unsaturated phosphatidylethanolamines in the case of the Ca2+-ATPase [14]. In connection with the present work on synthetic cardiolipins, it is also interesting to note that, in contrast to the bovine heart lipid, cardiolipin from yeast contains a high proportion of oleoyl chains, and 85% of the chains in CL from Acholeplasma laidlawii are composed of myristic and palmitic acids [15].…”

Influence of Lipid Components of Mycoplasma laidlawii Membranes on Osmotic Fragility of Cells

“…The fatty acid composition of M. laidlawii lipids apparently reflects the fatty acid composition of the growth medium, and accordingly it could be varied by addition of fatty acids to the medium (Tables 6, 7, and 8). The resemblance of the fatty acid composition of phospholipids of M. laidlawii to that of the growth medium was also noticed by Smith, Koostra, and Henrikson (27). The requirement of M. laidlawii for unsaturated fatty acids (21) suggests that this organism is incapable of synthesizing these acids.…”

Fatty acid composition, distribution, and requirements of two nonsterol-requiring mycoplasmas from complex but defatted growth media

“…The fatty acid content and composition of these cells, however, was not determined during these nutritional studies. Similarly, utilizing ill-defined but solvent-extracted media, Smith et al (1965) and Razin et al (1966b) showed the predominating presence of saturated over unsaturated acids in M. laidlawii phospholipids. However, as mentioned by Smith et al (1965) the significance of the fatty acid types found could not be assessed since the preextraction procedures employed did not remove all of the free fatty acids present in the culture medium.…”

“…Similarly, utilizing ill-defined but solvent-extracted media, Smith et al (1965) and Razin et al (1966b) showed the predominating presence of saturated over unsaturated acids in M. laidlawii phospholipids. However, as mentioned by Smith et al (1965) the significance of the fatty acid types found could not be assessed since the preextraction procedures employed did not remove all of the free fatty acids present in the culture medium. It has been shown that mycoplasmas, like bacteria, may mimic the fatty acid composition of the growth medium.…”