2006
DOI: 10.1530/rep.1.01216
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Diploid porcine parthenotes produced by inhibition of first polar body extrusion during in vitro maturation of follicular oocytes

Abstract: We investigated nuclear progression and in vitro embryonic development after parthenogenetic activation of porcine oocytes exposed to cytochalasin B (CB) during in vitro maturation (IVM). Nuclear progression was similar in control oocytes and oocytes matured in the presence of 1 mg/ml CB (IVM-CB group) by 37 h IVM; at this time the proportion of oocytes that had reached or passed through the anaphase-I stage did not differ significantly between the IVM-CB and the control groups (61.3 and 69.9% respectively; P!… Show more

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Cited by 24 publications
(19 citation statements)
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“…Although we did not analysis ploid of SCNT embryos, however, we believe that almost all these embryos are euploid, because we successfully produced live cloned offsprings using this procedure (Liu et al, ). Similar to our discoveries in nuclear transfer embryos, the double sets of chromosomes from anaphase‐I stage had reunited in 89.5% pig IVM‐CB oocytes after further culture of several hours (Somfai et al, ).…”
Section: Discussionsupporting
confidence: 86%
“…Although we did not analysis ploid of SCNT embryos, however, we believe that almost all these embryos are euploid, because we successfully produced live cloned offsprings using this procedure (Liu et al, ). Similar to our discoveries in nuclear transfer embryos, the double sets of chromosomes from anaphase‐I stage had reunited in 89.5% pig IVM‐CB oocytes after further culture of several hours (Somfai et al, ).…”
Section: Discussionsupporting
confidence: 86%
“…Heterozygous parthenotes were produced as described previously with minor modifications [8] by treating in vitro maturing oocytes with 5 µg/ml CB or 2.5 µg/ml CD at 20h of maturation for 22 hours (Figure 1). Oocytes were freed of cumulus cells by brief exposure to 0.1% hyaluronidase and manual pipetting and were activated 3 hours after treatment as previously described [16].…”
Section: Heterozygous Parthenotes Productionmentioning
confidence: 99%
“…While homozygous parthenotes have been produced for a range of species, heterozygous parthenotes have only been produced in mice and pigs [5,8,9]. With that heterozygous parthenotes derived from the oocytes of the same donor are practically a clones of donor of oocytes because have the same genome as donor of oocytes but with different gene arrangement because of cross over [9].…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…2005). Our recent data suggest that treatment of immature oocytes with cytochalasin B, a drug that inhibits actin filament polymerization, results in the failure of extrusion of the first polar body during the first meiosis (Somfai et al. 2006).…”
Section: Ivf and The Normality Of Porcine Zygotesmentioning
confidence: 99%