Diploid potato germplasm derived from wild and land race genetic resources

Watanabe, Kazuo; Orrillo, Matilde; Iwanaga, Michitaka; Ortíz, Rodomiro; Freyre, Rosanna; Perez, S.

doi:10.1007/bf02851525

Cited by 44 publications

(20 citation statements)

References 21 publications

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“…Thus, these diploids and tetraploids with Ry adg were deduced as heterozygous and simplex for Ry adg , respectively. Indeed, this inference was well correlated with the pedigree and progeny testing conducted previously (Watanabe et al 1992(Watanabe et al , 1994a, R. L. Plaisted, personal communication on NY clones in Table 1). As summarized in Figure 1, at least one R allele was found to be amplified from each Ry adg -carrying line, whereas all the ADG2 in non-Ry adg -carrying lines were amplified from the S allele with nucleotide C substituted by G. An exception was Pentland Crown, which had an allele with nucleotide G in the kinase-3a motif but many variations occurred in other regions.…”

Section: Adg2 Fragments Have Small But Specific Variationsupporting

confidence: 80%

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Conservation of a Resistance Gene-like Fragment ADG2 Related to Patato Y Potyvirus Resistance Gene Ryadg in Diploid and Tetrasomic Tetraploid Potato Lines

et al. 2003

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Section: Adg2 Fragments Have Small But Specific Variationsupporting

confidence: 80%

“…The sequence comparison also showed that the R alleles were well conserved among Ry adg carrying lines that derived from different genotypes of S. tuberosum ssp. andigena (Watanabe et al 1992(Watanabe et al , 1994a, R. L. Plaisted, personal communication on NY clones in Table 1). …”

Section: Adg2 Fragments Have Small But Specific Variationmentioning

confidence: 99%

Conservation of a Resistance Gene-like Fragment ADG2 Related to Patato Y Potyvirus Resistance Gene Ryadg in Diploid and Tetrasomic Tetraploid Potato Lines

et al. 2003

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“…These clones are 79-48 (genotype II RR pw ) (De Jong 1987), 320-02 (Ii Rr; unpublished), 07506-01 (ii rr) (De Jong 1987;De Jong and Burns 1983) and W5281.2 (Ii RR) (De Jong and Burns 1983). CIP590001.7 was described by Watanabe et al (1994). Diploids W5281.2, W5337.3 and W5295.7 were developed at the University of Wisconsin.…”

Section: Plant Materialsmentioning

confidence: 99%

An allele of dihydroflavonol 4-reductase associated with the ability to produce red anthocyanin pigments in potato (Solanum tuberosum L.)

Jong

et al. 2003

Theor Appl Genet

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The potato R locus is necessary for the production of red pelargonidin-based anthocyanin pigments in any tissue of the plant, including tuber skin and flower petals. The production of pelargonidins in plants requires the activity of dihydroflavonol 4-reductase (DFR) to catalyze the reduction of dihydrokaempferol into leucopelargonidin. To test the hypothesis that potato R encodes DFR, portions of both dfr alleles were sequenced from a diploid potato clone known to be heterozygous Rr. Sequence comparison revealed a polymorphic BamHI restriction site. The presence or absence of this site was monitored in three diploid populations that segregated for R, as well as in a wide range of tetraploid breeding clones and cultivars, by amplifying a fragment of dfr and digesting the products with BamHI. An identically sized dfr restriction fragment lacking the BamHI site was present in all potato clones that produced red anthocyanin pigments, while the same fragment was absent in many potato clones with white tuber skin and flowers. An independent RFLP test using DraI to detect sequence polymorphism was performed on a subset of the potato clones. This test also revealed dfr-derived bands that were present in all red-colored potatoes and absent in several white clones. The presence of shared restriction fragments in all red-colored potatoes provides strong evidence that R does indeed code for DFR. The data are also consistent with a 48 year-old hypothesis by Dodds and Long, that R was selected just once during the domestication of potato. A cDNA clone corresponding to the red allele of dfr was sequenced and compared to two other alleles. The red allele is predicted to encode a 382 amino acid protein that differs at ten amino acid positions from the gene products of the two alternative alleles. Several of these differences map in a region known to influence DFR substrate specificity in Gerbera.

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“…CIP made available this diploid potato germplasm bred with haploids, wild species, and landrace genetic resources (Watanabe et al 1994). The tetraploid potato cultivar "Nemared," derived from this diploid breeding population, was released in the 1990s in Burundi due to its bacterial wilt and root-knot nematode resistances and desired agronomic traits.…”

Section: Potatomentioning

confidence: 99%

Polyploidy and Plant Breeding

Ríos

2015

Plant Breeding in the Omics Era

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Diploid potato germplasm derived from wild and land race genetic resources

Cited by 44 publications

References 21 publications

Conservation of a Resistance Gene-like Fragment ADG2 Related to Patato Y Potyvirus Resistance Gene Ryadg in Diploid and Tetrasomic Tetraploid Potato Lines

Conservation of a Resistance Gene-like Fragment ADG2 Related to Patato Y Potyvirus Resistance Gene Ryadg in Diploid and Tetrasomic Tetraploid Potato Lines

An allele of dihydroflavonol 4-reductase associated with the ability to produce red anthocyanin pigments in potato (Solanum tuberosum L.)

Polyploidy and Plant Breeding

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