Diplotaxis harra and Diplotaxis simplex organs: Assessment of phenolics and biological activities before and after fractionation

Falleh, Hanen; Msilini, Najoua; Oueslati, Samia; Ksouri, Riadh; Magné, Christian; Lachâal, Mokhtar; Karray‐Bouraoui, Najoua

doi:10.1016/j.indcrop.2012.12.017

Cited by 22 publications

(41 citation statements)

References 35 publications

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“…3 | RESULTS AND DISCUSSION 3.1 | Quantification and identification of phenolics 3.1.1 | Total polyphenol, flavonoid, and proanthocyanidin contents Polyphenols constitute one of the major groups of compounds known for their antioxidant or free radical terminator abilities, which inhibit some molecular targets of pro-inflammatory mediators during inflammation (Fawole, Ndhlala, Amoo, Finnie, & Van, 2009). For these reasons, total phenols (TPC), flavonoids (TFC), and proanthocynidins (PC) have been determined in the studied plants (Table 1) (Falleh et al, 2013) and Salvia (Matkowski, Zielińska, Oszmiański, & Lamer-Zarawska, 2008). Our data showed that VO contained higher amounts of polyphenols than WT, which was more than previously reported values (TPC-35.4 mg/g and TFC-22.8 mg/g; Ebrahimzadeh et al, 2010).…”

Section: Discussionsupporting

confidence: 46%

“…For these reasons, total phenols (TPC), flavonoids (TFC), and proanthocynidins (PC) have been determined in the studied plants (Table ). VO and WT contained high amounts of TPC (48.18 and 29.79 mg GAE/g DW), TFC (18.78 and 8.16 mg QE/g DW) and PC (1.43 and 2.54 mg CE/g DW), as compared with other phenol‐rich medicinal plants like species of Diplotaxis (Falleh et al, ) and Salvia (Matkowski, Zielińska, Oszmiański, & Lamer‐Zarawska, ). Our data showed that VO contained higher amounts of polyphenols than WT, which was more than previously reported values (TPC—35.4 mg/g and TFC—22.8 mg/g; Ebrahimzadeh et al, ).…”

Section: Resultsmentioning

confidence: 99%

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Studies on antioxidant, hepatoprotective, and vasculoprotective potential of Viola odorata and Wrightia tinctoria

Jamshed

Siddiqi

Gilani

et al. 2019

Phytotherapy Research

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We have reported the antidyslipidemic, antihypertensive, and Ca++ channel blocking activities of Viola odorata (VO) and Wrightia tinctoria (WT). This study extends our understanding of their therapeutic potential by exploring the effects on biomarkers of hepatic and vascular dysfunction together with phytochemical standardization and antioxidant potential. Total phenolic compounds, total flavonoids content, and proanthocyanins of the methanolic extracts were identified using HPLC. Antioxidant capacity was measured using the in vitro assays. Two studies of 6‐week duration were conducted on a high‐fat diet rat model to test the leaves and seed extracts of VO and WT (300 and 600 mg/kg) for their effect on biomarkers for hepatic and vascular dysfunction. The HPLC analysis showed high contents of total phenolic compounds, total flavonoids content, and proanthocyanins along with distinctive phenolic composition. Both extracts exhibited significant antioxidant potential in 1,1‐diphenyl‐2‐picrylhydrazyl, 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid), fluorescence recovery after photobleaching, and total antioxidant capacity (TAC) assays, comparable with synthetic standard antioxidants. The in vivo studies indicated a significant reduction in the high‐fat‐diet‐induced rise in serum uric acid, phosphorus, aspartate aminotransferase, alanine aminotransferase, and gamma‐glutamyl transferase. This study indicates the potential of VO and WT to protect from vascular and hepatic damage and an antioxidant effect, thus making these herbs strong candidates for managing cardiometabolic disorders.

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Section: Discussionsupporting

confidence: 46%

Section: Resultsmentioning

confidence: 99%

Studies on antioxidant, hepatoprotective, and vasculoprotective potential of Viola odorata and Wrightia tinctoria

Jamshed

Siddiqi

Gilani

et al. 2019

Phytotherapy Research

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show abstract

“…Nevertheless, the highest flavonoids content was found in ethyl acetate extract (100.60 ± 3.4 mg QE/g extract). Falleh et al (2013) reported that the amount of phenolics present in Diplotaxis species exceeded those found in other medicinal plants. Besides, they showed that D. simplex flowers exhibited the highest polyphenolics and flavonoids contents with respect to leaves and stems of the plant.…”

Section: Resultsmentioning

confidence: 99%

“…HPLC analysis showed that the main phenolic compound identified in D. simplex flowers was caffeic acid. Furthermore, epigallocatechin, chlorogenic, p-coumaric and 3,4-dimethoxybenzoic acids were also identified in the plant flowers (Falleh et al 2013). After that, flower extracts were subjected to their antioxidant activities which were evaluated by complementary methods such as 2,2-diphenyl-1-picrylhydrazyl (DPPH ) radicalscavenging, reducing power, metal (Fe 2+ ) chelating and b-carotene bleaching assays (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Anti-inflammatory and anti-proliferative activities of the wild edible cruciferous:Diplotaxis simplex

Jdir

Khemakham

Najjaa

et al. 2016

Pharmaceutical Biology

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Context The present study deals with new biological properties of the wild edible Diplotaxis simplex (Viv.) Spreng (Brassicaceae). Objectives The current study evaluates the antioxidant, the anti-inflammatory and the anti-cancer properties of ethyl acetate and ethanol extracts from D. simplex flowers. Materials and methods The anti-proliferative activity of the extracts (10-70 mg/mL) was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) against human colon cancer cell line Caco-2. The anti-inflammatory potential was evaluated by the inhibitory effect of the extracts (1.5-7.5 mg/mL) on phospholipase A2 activity as well as on carrageenan-induced paw oedema in mice. Extracts (200 mg/kg) or indomethacin (50 mg/kg) as positive control were injected intraperitoneally for albino mice prior to the induction of the oedema by carrageenan. Antioxidant activities were investigated using various complementary methods.Results Flower extracts contained a high level of polyphenolics (17.10-52.70 mg GAE/g) and flavonoids (74.20-100.60 mg QE/g), which correlate with its appreciable antioxidant potential in b-carotene peroxidation (IC 50 value: 12.50-27.10 mg/mL), DPPH radical-scavenging (IC 50 value: 0.20-0.40 mg/mL), Fe 3+ reducing (EC 50 value: 0.10-0.14 mg/mL) and Fe 2+ chelating (IC 50 value: 0.20-0.60 mg/mL) assays. These extracts were effective in inhibiting cancer cell growth (IC 50 value: 62.0-63.25 mg/mL). Besides, the ethyl acetate extract inhibited phospholipase A2 activity (IC 50 value: 2.97 mg/mL) and reduced the paw oedema in mice (from 0.38 ± 0.01 to 0.24 ± 0.01 cm), 4 h postcarrageenan challenge. Conclusion These data suggest that D. simplex may be useful as a candidate in the treatment of inflammation and the colon cancer.ARTICLE HISTORY

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“…Indeed, D. harra is an edible plant used in traditional medicine for the treatment of various diseases such as anemia, hypercholesterolemia, diabetes and mainly inflammation and cancer (Bellakhdar 1997;Ghrabi 2005;Ramadan et al 2009). The aerial parts of this medicinal plant have a high polyphenol content that could support anti-inflammatory and anticancer properties (Falleh et al 2013;Kassem et al 2013). Our purpose was to determine if compounds from D. harra could modulate epithelial GSK3b activation in two in vitro models: PAR 2 -stimulated IEC6 cells, mimicking inflammatory conditions (Li et al 2015), and human colon cancerderived Caco-2 cell line grown in 3D.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory and anticancer effects of flavonol glycosides from Diplotaxis harra through GSK3β regulation in intestinal cells

Nasri

Chawech

Girardi

et al. 2016

Pharmaceutical Biology

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Diplotaxis harra and Diplotaxis simplex organs: Assessment of phenolics and biological activities before and after fractionation

Cited by 22 publications

References 35 publications

Studies on antioxidant, hepatoprotective, and vasculoprotective potential of Viola odorata and Wrightia tinctoria

Studies on antioxidant, hepatoprotective, and vasculoprotective potential of Viola odorata and Wrightia tinctoria

Anti-inflammatory and anti-proliferative activities of the wild edible cruciferous:Diplotaxis simplex

Anti-inflammatory and anticancer effects of flavonol glycosides from Diplotaxis harra through GSK3β regulation in intestinal cells

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