Direct 3D bioprinting of cardiac micro-tissues mimicking native myocardium

Liu, Justin; Miller, Kathleen L.; Ma, Xinwei; Dewan, Sukriti; Lawrence, Natalie; Whang, Grace; Chung, Peter; McCulloch, Andrew D.; Chen, Shaochen

doi:10.1016/j.biomaterials.2020.120204

Cited by 102 publications

(72 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chen et al . prepared CMs-laden GelMA and fabricated the cardiac microtissues using 3D bioprinting[ 28 ]. The phonotype of cells is similar with the cells in vivo .…”

Section: The Structures Of Heart-on-a-chipmentioning

confidence: 99%

“…fabricated similar microsensors by 3D bioprinting. They obtained microcantilevers with CMs-laden GelMA hydrogel and measured the contraction force[ 28 ].…”

Section: The Structures Of Heart-on-a-chipmentioning

confidence: 99%

“…fabricated the 3D microtissues using 3D bioprinting, and monitored the concentration of calcium. They found that the isopropylnoradrenaline can affect the calcium concentration[ 28 ]. Zhao et al .…”

Section: Biomedical Applications Of Heart-on-a-chipmentioning

confidence: 99%

See 2 more Smart Citations

Fabrication and Biomedical Applications of Heart-on-a-chip

Yang

Xiao

et al. 2024

IJB

View full text Add to dashboard Cite

Heart diseases have become the main killer threatening human health, and various methods have been developed to study heart disease. Among them, heart-on-a-chip has emerged in recent years as a method for constructing disease (or normal) models in vitro and is considered as a promising tool to study heart diseases. Compared with other methods, the advantages of heart-on-a-chip include the high portability, high throughput, and the capability to mimic microenvironments in vivo. It has shown a great potential in disease mechanism study and drug screening. In this paper, we review the recent advances in heart on-a-chip, including the fabrication methods (e.g., 3D bioprinting) and biomedical applications. By analyzing the structure of the existing heart-on-a-chip, we proposed that a highly integrated heart-on-a-chip includes four elements: Microfluidic chips, cells/microtissues, microactuators to construct the microenvironment, and microsensors for results readout. Finally, the current challenges and future directions of heart-on-a-chip are discussed.

show abstract

“…Chen et al . prepared CMs-laden GelMA and fabricated the cardiac microtissues using 3D bioprinting[ 28 ]. The phonotype of cells is similar with the cells in vivo .…”

Section: The Structures Of Heart-on-a-chipmentioning

confidence: 99%

“…fabricated similar microsensors by 3D bioprinting. They obtained microcantilevers with CMs-laden GelMA hydrogel and measured the contraction force[ 28 ].…”

Section: The Structures Of Heart-on-a-chipmentioning

confidence: 99%

See 1 more Smart Citation

Fabrication and Biomedical Applications of Heart-on-a-chip

Yang

Xiao

et al. 2024

IJB

View full text Add to dashboard Cite

show abstract

“…Bioinks are often hydrogels containing live cells in a suitable cell culture medium [ 199 , 200 ]. Hydrogels are the principal materials applied as bioinks for the regeneration of neural [ 201 ], cardiac [ 202 ], cartilage [ 203 ], and skin [ 204 ] tissues.…”

Section: Processing Polysaccharide-based Materials For Biomedical Applicationsmentioning

confidence: 99%

Polysaccharide-Based Materials Created by Physical Processes: From Preparation to Biomedical Applications

et al. 2021

View full text Add to dashboard Cite

Polysaccharide-based materials created by physical processes have received considerable attention for biomedical applications. These structures are often made by associating charged polyelectrolytes in aqueous solutions, avoiding toxic chemistries (crosslinking agents). We review the principal polysaccharides (glycosaminoglycans, marine polysaccharides, and derivatives) containing ionizable groups in their structures and cellulose (neutral polysaccharide). Physical materials with high stability in aqueous media can be developed depending on the selected strategy. We review strategies, including coacervation, ionotropic gelation, electrospinning, layer-by-layer coating, gelation of polymer blends, solvent evaporation, and freezing–thawing methods, that create polysaccharide-based assemblies via in situ (one-step) methods for biomedical applications. We focus on materials used for growth factor (GFs) delivery, scaffolds, antimicrobial coatings, and wound dressings.

show abstract

“…Historically, translational designs for a regenerative cardiac patch have been structured by isotropic scaffolds or gels [41][42][43] . Recent improvements in scaffold fabrication technology have resulted in increasingly refined control over three dimensional (3D) scaffold architecture and subsequently the identification of biomimetic structure as an effective functional modulator 17,30,[44][45][46][47] . While these studies support structural anisotropy as a modulator of engineered cardiac tissue function, due to the complexity of scaffold fabrication and its interdependent relationship with both micro-and microarchitecture the isolated functional and developmental effects of scaffold structure are not fully understood.…”

Section: Introductionmentioning

confidence: 99%

Unravelling Form and Function: Improved function of engineered cardiac tissue through extra-cellular anisotropy

Cyr

Colzani

Bayraktar

et al. 2021

Preprint

View full text Add to dashboard Cite

Cardiac tissue engineering is a promising therapeutic option for myocardial repair after injury, however, so far engineered heart patches have shown limited translational utility due to poor electrical integration and tissue contractility. Emerging research suggests that scaffolds that recapitulate the three-dimensional structure of the native myocardium improve physiological function. Complex scaffold fabrication remains a technical challenge and the isolated impact of scaffold architecture on tissue function and cellular physiology is poorly understood. Here, we provided a direct comparison between isotropic and aligned collagen scaffold morphologies where all confounding physio-mechanical features, such as strut wall thickness and surface roughness are conserved. This enabled the independent and systematic assessment of the effects of pore macro-architecture on global tissue function and cellular maturation. We seeded our scaffolds with embryonic stem cell derived cardiomyocytes (hESC-CM) and measured tissue function through calcium signal transduction and dynamic contractile strain. The aligned tissue constructs facilitated improved signalling synchronicity and directional contractility. We further examined the influence of scaffold macrostructure on intercellular organization and intracellular development. Cells on aligned constructs conformed to the orientation of the scaffold macro-structure and were found to have phenotypic and genetic markers of increased maturity. Our results isolate the influence of scaffold macro-structure on engineered tissue function at multiple length scales. These findings inform the design of optimized cardiac tissue and expand the potential for engineered tissue in regenerative and model medical systems by reducing the gaps in tissue functionality that limit their utility.

show abstract

Direct 3D bioprinting of cardiac micro-tissues mimicking native myocardium

Cited by 102 publications

References 40 publications

Fabrication and Biomedical Applications of Heart-on-a-chip

Fabrication and Biomedical Applications of Heart-on-a-chip

Polysaccharide-Based Materials Created by Physical Processes: From Preparation to Biomedical Applications

Unravelling Form and Function: Improved function of engineered cardiac tissue through extra-cellular anisotropy

Contact Info

Product

Resources

About