Direct acetylation for full analysis of polysaccharides in edible plants and fungi using reverse phase liquid chromatography-multiple reaction monitoring mass spectrometry

Gao, Jia-Ning; Li, Ye; Liang, Jun; Chai, Jun-Hong; Kuang, Hai‐Xue; Xia, Yueyuan

doi:10.1016/j.jpba.2022.115083

Cited by 7 publications

(9 citation statements)

References 34 publications

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“…Monosaccharide composition analysis is the basis for studying the structural properties and the structure–activity relationships of polysaccharides. Common analytical methods for determining monosaccharide composition currently include liquid chromatography [ 10 ], liquid chromatography–mass spectrometry ( Figure 8 ) [ 97 ], gas chromatography [ 64 ] and gas chromatography–mass spectrometry [ 41 ]. A large number of studies have concluded that the chemical modification of natural polysaccharides generally leads to changes in the molar percentage of monosaccharide composition, but it does not lead to changes in monosaccharide composition [ 42 , 64 , 77 , 82 ].…”

Section: Factors Affecting the Bioactivity Of Chemically Modified Pol...mentioning

confidence: 99%

“…b1 : Ganoderma lucidum polysaccharide; b2 : Auricularia auricular-judae polysaccharide; b3 : Armillaria mellea polysaccharide; b4 : Hericium erinaceus polysaccharide; b5 : Panax ginseng polysaccharide; b6 : Aralia elata bud polysaccharide; b7 : Platycodon grandiflorum polysaccharide; b8 : Stigma maydis polysaccharide. This figure was adapted from Gao et al [ 97 ].…”

Section: Figurementioning

confidence: 99%

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Chemical Modification of Polysaccharides: A Review of Synthetic Approaches, Biological Activity and the Structure–Activity Relationship

et al. 2023

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Natural polysaccharides are macromolecular substances with great potential owing to their wide biological activity and low toxicity. However, not all polysaccharides have significant pharmacodynamic activity; hence, appropriate chemical modification methods can be selected according to the unique structural characteristics of polysaccharides to assist in enhancing and promoting the presentation of their biological activities. This review summarizes research progress on modified polysaccharides, including common chemical modification methods, the change in biological activity following modification, and the factors affecting the biological activity of chemically modified polysaccharides. At the same time, the difficulties and challenges associated with the structural modification of natural polysaccharides are also outlined in this review. Thus, research on polysaccharide structure modification is critical for improving the development and utilization of sugar products.

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Section: Factors Affecting the Bioactivity Of Chemically Modified Pol...mentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Chemical Modification of Polysaccharides: A Review of Synthetic Approaches, Biological Activity and the Structure–Activity Relationship

et al. 2023

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“…However, these methods involve multiple enzymes, increasing the complexity and cost . Additionally, mass spectrometry has been designed to identify acetylation substrate residues, but it requires expensive instrument and complicated procedures . Various immunoassays have also been developed by combining with different biosensing mechanisms, for example, chemiluminescence, fluorescence resonance energy transfer, and colorimetric assays. − However, the high cost and unsatisfactory sensitivity remain significant barriers, especially when analyzing low-abundance acetylation-related enzymes in complex microenvironments.…”

Section: Introductionmentioning

confidence: 99%

Portable Detection of Lysine Acetyltransferase Activity in Lung Cancer Cells Based on a Miniature Electrochemical Sensor

Liu,

Lu,

et al. 2024

Anal. Chem.

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The detection of lysine acetyltransferases is crucial for diagnosing and treating lung cancer, highlighting the necessity for highly efficient detection methods. We developed a portable, highly accurate, and sensitive technique using fast-scan cyclic voltammetry (FSCV) to determine the activity of the lysine acetyltransferase TIP60, employing a novel miniature electrochemical sensor. This approach involves a compact electrochemical cell, merely 3 mm in diameter, that holds solutions up to 50 μL, equipped with a conductive indium tin oxide working electrode. Uniquely, this system operates on a two-electrode model compatible with the FSCV, obviating the traditional requirement for a reference electrode. The system detects TIP60 activity through the continuous generation of CoA molecules that engage in reactions with Cu(II), thereby significantly improving the accuracy of the acetylation analysis. Remarkably, the detection limit achieved for TIP60 is notably low at 3.3 pg/mL (S/N = 3). The results show that the reversible dynamic acetylation can be effectively regulated by inhibitor incubation and glucose stimulation. This cutting-edge strategy enhances the analysis of a broad spectrum of biomarkers by modifying the responsive unit, and its miniaturization and portability significantly amplify its applicability in biomedical research, promising it to be a versatile tool for early diagnostic and therapeutic interventions in lung cancer.

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“…The process often involves full monosaccharide liberation, derivative reaction and qualitative or quantitative elucidation of the derivatives with available separation and detection techniques, among which GC–MS is one of the mainstream methods for the derivatization identification of monosaccharides (Ruiz‐Matute et al, 2011; Xia et al, 2018). Based on the previous reports, aldononitrile acetylation, alditol acetylation, direct acetylation and other analytical methods have been diffusely employed for analyzing monosaccharide components by GC–MS (Gao et al, 2023; Liang et al, 2011; Wang et al, 2007). Silylation and trifluoroacetylation, both also combined with prior oximation, have also been reported for GC analysis (Konig et al, 1988; Rojas‐Escudero et al, 2004; Sullivan & Schewe, 1977; Zweckmair et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Development of a joint derivatization protocol for the unequivocal identification of the monosaccharide composition in four dendrobium polysaccharides and free monosaccharide by GC–MS

Yang,

Hao,

Chen

et al. 2023

Biomedical Chromatography

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The determination of monosaccharides is crucial for studying the structure of polysaccharides and the composition of free monosaccharides in living organisms. Based on previous derivatization gas chromatography–mass spectrometry (GC–MS) methods, we aimed to develop a novel analytical protocol for better quantifying monosaccharides. In this study, sugar alcohol acetylation, saccharonitrile acetylation, silylation and a combination of sugar alcohols acetylation and saccharonitrile acetylation were compared. The optimal method was verified with the monosaccharide determination of four polysaccharides and four free monosaccharides from Dendrobium. The results showed that the novel combined derivatization method was superior to the other three methods in terms of content analysis of monosaccharides. Furthermore, it possessed good linearity (all calibration curves showed relative coefficients ≥ 0.999), sensitivity, precision (relative standard deviation < 2%), and accuracy (recovery, 95.7–105%). Finally, the novel method established in this study was successfully employed in determining the monosaccharide composition of four polysaccharides and four free monosaccharide samples from Dendrobium.

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Direct acetylation for full analysis of polysaccharides in edible plants and fungi using reverse phase liquid chromatography-multiple reaction monitoring mass spectrometry

Cited by 7 publications

References 34 publications

Chemical Modification of Polysaccharides: A Review of Synthetic Approaches, Biological Activity and the Structure–Activity Relationship

Chemical Modification of Polysaccharides: A Review of Synthetic Approaches, Biological Activity and the Structure–Activity Relationship

Portable Detection of Lysine Acetyltransferase Activity in Lung Cancer Cells Based on a Miniature Electrochemical Sensor

Development of a joint derivatization protocol for the unequivocal identification of the monosaccharide composition in four dendrobium polysaccharides and free monosaccharide by GC–MS

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