Direct acetylation of a conserved threonine of RIN4 by the bacterial effector HopZ5 or AvrBsT activates RPM1-dependent immunity in Arabidopsis

Choi, Si Hwan; Prokchorchik, Maxim; Lee, Hyeonjung; Gupta, Ravi; Lee, Yoonyoung; Chung, Eui Hwan; Cho, Buhyeon; Kim, Min Sung; Kim, Sun Tae; Sohn, Kee Hoon

doi:10.1016/j.molp.2021.07.017

Cited by 35 publications

(41 citation statements)

References 58 publications

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“…2 and 3), NbPtr1 is activated upon the recognition of AvrRpt2, AvrRpm1, AvrB, HopZ5, and AvrBsT. Since the effectors that are recognized by NbPtr1 are previously reported to modify a guardee called RIN4 in A. thaliana (Axtell & Staskawicz, 2003; Mackey et al ., 2003; Day et al ., 2005; Chung et al ., 2011; Liu et al ., 2011; Redditt et al ., 2019; Mazo-Molina et al ., 2020; Choi et al ., 2021), we tested if N. benthamiana RIN4 homologs are required for the effector-induced PCD (Fig. S6).…”

Section: Resultsmentioning

confidence: 99%

“…DNA fragments of VIGS-com flanked by BsaI sites were synthesized (Twist Biosciences, USA) and cloned into the pUC19b vector (Jayaraman et al ., 2017). Subsequently, VIGS-com were assembled into a Golden Gate compatible pTRV2-66 (Choi et al ., 2021). All VIGS constructs in addition to VIGS-com cloned in this study were assembled into pTRV2-66 (Choi et al ., 2021).…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, VIGS-com were assembled into a Golden Gate compatible pTRV2-66 (Choi et al ., 2021). All VIGS constructs in addition to VIGS-com cloned in this study were assembled into pTRV2-66 (Choi et al ., 2021).…”

Section: Methodsmentioning

confidence: 99%

“…MACULICOLA 1 (RPM1) (Chung et al, 2011;Liu et al, 2011;Redditt et al, 2019). Similarly, Pseudomonas and Xanthomonas effectors, HopZ5 and AvrBsT, respectively, acetylate Thr166 and activate RPM1 (Choi et al, 2021). Lastly, the Pseudomonas effector AvrRpt2 cleaves RIN4 at two major sites leading to the activation of the CNL protein RESISTANCE TO P. SYRINGAE 2 (RPS2) in Arabidopsis Mackey et al, 2003;Day et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Ptr1 and ZAR1 immune receptors confer overlapping and distinct bacterial pathogen effector specificities

Ahn

Kim

Choi

et al. 2022

Preprint

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Nucleotide-binding and leucine-rich repeat receptors (NLRs) detect pathogen effectors inside the plant cell. To identify Nicotiana benthamiana NLRs (NbNLRs) with novel effector recognition specificity, we designed an NbNLR VIGS library and conducted a rapid reverse genetic screen. During the NbNLR VIGS library screening, we identified that N. benthamiana homolog of Ptr1 (PSEUDOMONAS SYRINGAE PV. TOMATO RACE 1 RESISTANCE) recognizes the Pseudomonas effectors AvrRpt2, AvrRpm1, and AvrB. We demonstrated that recognition of the Xanthomonas effector AvrBsT and the Pseudomonas effector HopZ5 in N. benthamiana is conferred independently by N. benthamiana homolog of Ptr1 and ZAR1 (HOPZ-ACTIVATED RESISTANCE 1). In addition, we showed that the RLCK XII family protein JIM2 (XOPJ4 IMMUNITY 2) physically interacts with AvrBsT and HopZ5 and is required for the NbZAR1-dependent recognition of AvrBsT and HopZ5. The recognition of multiple bacterial effectors by Ptr1 and ZAR1 in N. benthamiana demonstrates a convergent evolution of effector recognition across plant species. Identification of key components involved in Ptr1 and ZAR1 mediated immunity would reveal unique mechanisms of expanded effector recognition and be useful for engineering resistance in solanaceous crops.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ptr1 and ZAR1 immune receptors confer overlapping and distinct bacterial pathogen effector specificities

Ahn

Kim

Choi

et al. 2022

Preprint

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“…Additional research has focused on the recognition mechanism of Psa T3SEs in model plants. HopZ5 Psa -tiggered ETI can be inhibited by SOBER1 in non-host Arabidopsis, while AvrRpm1 Psa can be recognized by Rpa1 (but not RPM1) to trigger a plant immunity response [ 30 , 31 , 32 , 33 ]. Other than HopZ5 and AvrRpm1, reports of Psa T3SEs that can activate plant immune responses are limited.…”

Section: Introductionmentioning

confidence: 99%

Pseudomonas syringae pv. actinidiae Effector HopAU1 Interacts with Calcium-Sensing Receptor to Activate Plant Immunity

Zhang

Zhou

Liu

et al. 2022

IJMS

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Kiwifruit canker, caused by Pseudomonas syringae pv. actinidiae (Psa), is a destructive pathogen that globally threatens the kiwifruit industry. Understanding the molecular mechanism of plant-pathogen interaction can accelerate applying resistance breeding and controlling plant diseases. All known effectors secreted by pathogens play an important role in plant-pathogen interaction. However, the effectors in Psa and their function mechanism remain largely unclear. Here, we successfully identified a T3SS effector HopAU1 which had no virulence contribution to Psa, but could, however, induce cell death and activate a series of immune responses by agroinfiltration in Nicotiana benthamiana, including elevated transcripts of immune-related genes, accumulation of reactive oxygen species (ROS), and callose deposition. We found that HopAU1 interacted with a calcium sensing receptor in N. benthamiana (NbCaS) as well as its close homologue in kiwifruit (AcCaS). More importantly, silencing CaS by RNAi in N. benthamiana greatly attenuated HopAU1-triggered cell death, suggesting CaS is a crucial component for HopAU1 detection. Further researches showed that overexpression of NbCaS in N. benthamiana significantly enhanced plant resistance against Sclerotinia sclerotiorum and Phytophthora capsici, indicating that CaS serves as a promising resistance-related gene for disease resistance breeding. We concluded that HopAU1 is an immune elicitor that targets CaS to trigger plant immunity.

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Polyamines and Their Crosstalk with Phytohormones in the Regulation of Plant Defense Responses

Asija

Seth

Umar

et al. 2022

J Plant Growth Regul

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Direct acetylation of a conserved threonine of RIN4 by the bacterial effector HopZ5 or AvrBsT activates RPM1-dependent immunity in Arabidopsis

Cited by 35 publications

References 58 publications

Ptr1 and ZAR1 immune receptors confer overlapping and distinct bacterial pathogen effector specificities

Ptr1 and ZAR1 immune receptors confer overlapping and distinct bacterial pathogen effector specificities

Pseudomonas syringae pv. actinidiae Effector HopAU1 Interacts with Calcium-Sensing Receptor to Activate Plant Immunity

Polyamines and Their Crosstalk with Phytohormones in the Regulation of Plant Defense Responses

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