Direct ammonium oxidation to nitrogen gas (Dirammox) in Alcaligenes strain HO-1: The electrode role

Pous, Narcís; Bañeras, Lluís; Corvini, Philippe F.-X.; Liu, Shuang‐Jiang; Puig, Sebastià

doi:10.1016/j.ese.2023.100253

Cited by 10 publications

(4 citation statements)

References 29 publications

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“…For instance, it has been suggested that the A. faecalis strain HP8 can biodegrade anthracene in soil [35], A. faecalis JQ191 may do the same for quinolinic acid [36], and A. faecalis RB-10 can do the same for indole [37]. A. faecalis strains like HO-1 have the ability to directly oxidize ammonium to nitrogen gas (Dirammox) [38]. Simultaneous ammonia nitrogen and organic carbon removal in a biofilm reactor was achieved after bioaugmentation with A. faecalis strain NR The introduction of new bacterial cells and intense aeration, which led to some cells failing to form a colony on the agar media, are likely to be responsible for the microbial titer in the experimental samples decreasing one day after the experiment began.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Use of Alcaligenes faecalis to Reduce Coliforms and Enhance the Stabilization of Faecal Sludge

Loiko,

Kanunnikov,

Litti

2023

Sustainability

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The disposal of human faecal sludge (FS) is one of the biggest environmental problems. It can be solved by using FS as an agricultural fertilizer. However, this is hampered by the presence of pathogenic microflora and high organic matter content in FS. This paper proposes a novel treatment approach for FS to address these issues. It consists of the preliminary aerobic incubation of FS with the bioaugmentation of bacterial culture Alcaligenes faecalis DOS7. A. faecalis has been shown to inhibit the growth of various microorganisms, including coliforms (Escherichia coli). For the treatment of FS, three tanks with a volume of 1 m3 each, equipped with a mixing and aeration system, were used. A. faecalis culture was introduced into two experimental tanks at a concentration of 106 and 6.5 × 106 cells/mL. The 11-day incubation in the experimental tanks resulted in the decomposition of organic matter in the FS that was several times faster than in the control (p < 0.05). Total suspended solids decreased 2.5–5-fold, chemical oxygen demand decreased 1.8-fold, 5-day biochemical oxygen demand decreased 1.5–2-fold. At the same time, after 4 days of incubation, no coliforms were detected in the experimental tanks, and in the control, coliforms accounted for 13.9% of the total number of cells after 11 days of incubation. The proposed method of FS pretreatment is a real alternative to the existing ones and can be used both individually and in combination with other methods, for example, composting.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Use of Alcaligenes faecalis to Reduce Coliforms and Enhance the Stabilization of Faecal Sludge

Loiko,

Kanunnikov,

Litti

2023

Sustainability

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“…The overexpression of amoA genes compared to denitrification-related genes suggests the potential existence of a direct ammonia oxidation pathway (NH 4 + →NH 2 OH→N 2 ). This direct ammonia oxidation reaction utilizes ammonia as an electron donor and O 2 as an electron acceptor, transforming ammonia directly to N 2 via NH 2 OH as an intermediate [38]. Unlike the common complete nitrification-denitrification process, the direct ammonia oxidation process significantly reduces the consumption of electrons, demonstrating immense application potential in the field of biological nitrogen removal [39].…”

Section: Different Rotational Speedsmentioning

confidence: 99%

Characteristics of Nitrogen Removal and Functional Gene Transcription of Heterotrophic Nitrification-Aerobic Denitrification Strain, Acinetobacter sp. JQ1004

Hou,

Huang,

Pan

et al. 2024

Water

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In this study, the heterotrophic nitrification–aerobic denitrification strain JQ1004 was investigated in terms of its nitrogen removal mechanism and kinetic properties, laying the foundation for its application in the field of wastewater treatment. Nitrogen balance analysis revealed that the final metabolic product was N2, and approximately 54.61% of N was converted into cellular structure through assimilation. According to the fitting of the Compertz model, the maximum degradation rates of ammonia and nitrate were 7.93 mg/(L·h) and 4.08 mg/(L·h), respectively. A weakly alkaline environment was conducive to N removal, and the sensitivity of functional genes to acidic environments was amoA > nirS > narG. An appropriate increase in dissolved oxygen significantly enhanced heterotrophic nitrification activity, and notably, the denitrification-related functional gene narG exhibited greater tolerance to dissolved oxygen compared to nirS. The transcription level of amoA was significantly higher than that of narG or nirS, confirming that there might have been direct ammonia oxidation metabolic pathways (NH4+→NH2OH→N2) besides the complete nitrification and denitrification pathway. The annotation of nitrogen assimilation-related functional genes (including gltB, gltD, glnA, nasA, nirB, narK, nrtP, cynT, and gdhA genes) in the whole-genome sequencing analysis further confirmed the high assimilation nitrogen activity of the HN-AD strain.

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“…A recent study demonstrated that Dirammox bacteria can employ polarized electrodes as terminal electron acceptors instead of oxygen, further reducing the aeration requirement. 9 Moreover, the Dirammox process may need fewer electron donors compared to the denitrification process, which require 3 and 5 mol of e − from the carbon source to reduce 1 mol of nitrite and nitrate, respectively. In contrast, the Dirammox process utilizes ammonia as an electron donor, eliminating the need to obtain electrons from carbon sources for nitrogen conversion.…”

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confidence: 99%

Tapping the Potential of Wastewater Treatment with Direct Ammonia Oxidation (Dirammox)

Pan

Liu

2023

Environ. Sci. Technol.

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Direct ammonium oxidation to nitrogen gas (Dirammox) in Alcaligenes strain HO-1: The electrode role

Cited by 10 publications

References 29 publications

Use of Alcaligenes faecalis to Reduce Coliforms and Enhance the Stabilization of Faecal Sludge

Use of Alcaligenes faecalis to Reduce Coliforms and Enhance the Stabilization of Faecal Sludge

Characteristics of Nitrogen Removal and Functional Gene Transcription of Heterotrophic Nitrification-Aerobic Denitrification Strain, Acinetobacter sp. JQ1004

Tapping the Potential of Wastewater Treatment with Direct Ammonia Oxidation (Dirammox)

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