Direct amperometric determination of lipase activity

Computational Modeling of the Amperometric Bioanalytical System for Lipase Activity Assay: a Time-Dependent Response

“…In a simplified one-dimension model (Fig. 1), the working space of bioelectroanalytical system described in [1] could be divided into two parts: the first one -wide area, where enzymatic reaction and molecular/particle (convective-)diffusion occur, the second one -narrow area of a diffusion layer, where the diffusion of hydrolysis product occurs. The latter model assumes that area 2 experimentally could be made inaccessible (e.g., by covering the electrode surface with dialysis membrane) for other components of the system.…”

“…Recently, the amperometric detection method of Thermomyces lanuginosus lipase activity has been published [1]. Lipases, triacylglycerol hydrolases (EC 3.1.1.3) that cleave triacylglycerols at the oil/water interface, have extensive applications in the food, paper, pharmaceutical, cosmetic, detergent, leather, and textile industries [2,3].…”

“…Widespread practical use of these enzymes requires fast and reliable analytical routines to assess their activity. The electrochemical technique, described in [1], presents the method of this kind.…”

“…The PDCHQ molecules were solubilized in the Triton X-100 micelles, while the product of enzymatic hydrolysis, 2,3-dicyanohydroquinone, was readily oxidized on the electrode in a diffusion-controlled process. Under the diffusion control, the magnitude of the electrode current is determined solely by the concentration and diffusion coefficient of the electroactive species (in the case of work [1], 2,3-dicyanohydroquinone) and the effective thickness of the diffusion layer [4].…”

“…The authors of the paper [1] have performed experiments under the steady-state conditions. The aim of the present work is computational modeling of response kinetics of this bioelectroanalytical system.…”