Direct Analysis of Dried Blood Spot Samples

Abu-Rabie, Paul

doi:10.1002/9781118890837.ch22

Cited by 7 publications

(11 citation statements)

References 184 publications

(267 reference statements)

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“…The above control data were also collected using direct elution. Direct elution was carried out using the CAMAG DBS-MS500, which is one of the few commercially available DBS direct elution instruments . This instrument fully automates the DBS direct elution process first reported in 2009 .…”

Section: Resultsmentioning

confidence: 99%

“…This is a more refined version of the IS spray technique we previously reported . This IS module eliminates the “flooding” issues we experienced during our previous report by replacing the static application used with the Touchspray device (The Technology Partnership, Hertfordshire, U.K.) with a dynamic spray application. , Rather than applying the IS solution in a single spray arc covering the entire DBS, this system applies a much finer stream, covering only a very small section of the sample (spray arc diameter less than 2 mm) and uses automation to move the sample relative to the spraying device (a dynamic spray application). The result is a 10 × 10 mm grid of IS solution applied centered around the DBS (guided by the integrated visual recognition system).…”

Section: Resultsmentioning

confidence: 99%

“…Direct elution was carried out using the CAMAG DBS-MS500, which is one of the few commercially available DBS direct elution instruments. 23 This instrument fully automates the DBS direct elution process first reported in 2009. 24 Further work has shown this technique to be suitably reliable and reproducible, and it is used here as an example of how to make the DBS workflow more efficient and reduce the additional manual burden associated with DBS.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In addition to the issues associated with HCT, another notable barrier to introducing DBS analysis to pharmaceutical drug development was persuading bioanalysts to accept a technique that was new and both more complex, and offered less assay sensitivity than the conventional wet plasma analysis technique it was potentially replacing . To counter this, numerous groups have investigated DBS direct analysis techniques that eliminate manual preparation prior to analysis and (in some cases) enable increased assay sensitivity through injecting the entire extracted volume onto the analytical system. ,, We have found that the most accessible DBS direct analysis technique for use in regulated quantitative bioanalysis is currently direct elution, as it offers reliable performance, compatibility with HPLC, and increased assay sensitivity. ,, In order to maximize the scope of this work, for each method of IS addition, analysis was investigated using both conventional manual extraction (using both subpunch and whole-spot extraction) and direct elution (using both sub sampling and whole-spot elution).…”

mentioning

confidence: 99%

“…4,18,23 We have found that the most accessible DBS direct analysis technique for use in regulated quantitative bioanalysis is currently direct elution, as it offers reliable performance, compatibility with HPLC, and increased assay sensitivity. 4,23,24 In order to maximize the scope of this work, for each method of IS addition, analysis was investigated using both conventional manual extraction (using both subpunch and whole-spot extraction) and direct elution (using both sub sampling and whole-spot elution).…”

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confidence: 99%

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Investigation of Different Approaches to Incorporating Internal Standard in DBS Quantitative Bioanalytical Workflows and Their Effect on Nullifying Hematocrit-Based Assay Bias

et al. 2015

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Hematocrit (HCT)-based assay bias (composed of area and recovery bias) is an important contributing factor to the barriers that currently hinder the development and acceptance of dried blood spots (DBS) as a widely used quantitative bioanalytical sampling technique for regulatory studies. This article describes the evaluation of a practical internal standard spray addition technique, used prior to LC-MS/MS analysis, which is demonstrated to nullify the effect of recovery bias. To our knowledge, this is the first time a potential solution to HCT-based recovery bias has been investigated in detail and reported in the literature. This new technique is coupled with accurate volume DBS sampling, whole-spot extraction, and automated direct elution techniques to demonstrate a workflow that both nullifies HCT-based assay bias and the additional manual extraction burden associated with DBS analysis.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Investigation of Different Approaches to Incorporating Internal Standard in DBS Quantitative Bioanalytical Workflows and Their Effect on Nullifying Hematocrit-Based Assay Bias

et al. 2015

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Harmonization of distributed multi‐center analysis based on dried blood spot reference materials supporting the screening of neonatal inherited metabolic disorders

Qu,

Tao,

Qin

et al. 2023

Clinical Laboratory Analysis

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BackgroundThe standardization of quantification data is critical for ensuring the reliability and measurement traceability in the screening of neonatal inherited metabolic disorders. However, the availability of national certified reference materials is limited in China.MethodsIn this study, we developed a series of dried blood spot (DBS) reference materials containing 9 amino acids (AA) and 10 acylcarnitines (AC) for neonatal screening. Four levels of the reference materials were measured with tandem mass spectrometry (MS/MS) by seven laboratories using different commercial In Vitro Diagnostic Device (IVD) kits. Then, 100 clinical samples were measured using both derivatization and non‐derivatization methods by the same laboratory.ResultsWe found high homogeneity and stability at all levels of the reference materials, with the coefficient of variation (CV) of the analytes less than 15%. These reference materials can be used to assess the testing capabilities of different laboratories. Our test also revealed that the correction factors (CF) calculated by the reference materials, along with clinical samples, could increase the consistency for different kits.ConclusionThe DBS reference materials proposed in this study provide reliability for the harmonization in multi‐center analysis for the screening of neonatal inherited metabolic disorders. And applying our correction method for the screening could improve the data consistency of the DBS samples prepared by different methods.

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The Open SprayBot: A high-throughput paper spray mass spectrometry platform for disease screening

García-Rojas,

Guillén-Alonso,

MacKay

et al. 2024

HardwareX

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Direct Analysis of Dried Blood Spot Samples

Cited by 7 publications

References 184 publications

Investigation of Different Approaches to Incorporating Internal Standard in DBS Quantitative Bioanalytical Workflows and Their Effect on Nullifying Hematocrit-Based Assay Bias

Investigation of Different Approaches to Incorporating Internal Standard in DBS Quantitative Bioanalytical Workflows and Their Effect on Nullifying Hematocrit-Based Assay Bias

Harmonization of distributed multi‐center analysis based on dried blood spot reference materials supporting the screening of neonatal inherited metabolic disorders

The Open SprayBot: A high-throughput paper spray mass spectrometry platform for disease screening

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