Direct analysis of root zone data in a microculture system

Abstract

The feasibility of using a whole plant microculture system coupled with image analysis to observe and quantify elusive root growth phenomena was demonstrated. Subtle differences in root initiation and growth rate for maple microcuttings inserted into three distinct rooting media were recurrently registered over the span of the rooting phase in terms of root length, number, and weighted density (equivalent to fresh weight) without disturbing the rooting environment. This method provided a non-intrusive time-course assay for take-all disease symptom expression on wheat lines (degree and rate of root lesion development), which paralleled the relative resistance rated in field plots. Although agar-solidified media had a relatively high light transmission (nearly 84% through 6.6 cm medium depth), roots could only be clearly perceived through a thickness of about 2 cm, and were often completely obscured when embedded to a depth greater than 5 cm.

Increased Phosphorus Mitigates the Adverse Effects of Salinity in Tissue Culture

“…In vitro cultures could be an effective alternative to avoid soil or environmental complexities when studying plant response to an imposed stress factor (11). In vitro cultures have the advantage of small scale and treatment control, with clear visibility for monitoring shoot and root responses in the presence of the imposed stress (11,15,16,17,18). Therefore; results of in vitro system, yielded useful information to elucidate plant response to stress for the study mechanism of plant stress differences (11).…”

Growth, Osmotic Adjustment, and Nutrient Acquisition of Bitter Almond Under Induced Sodium Chloride Salinity In Vitro

“…[2,5,9,11] In vitro culture system has been adapted for studying salinity responses in different plant species. [2,9,11,13,14] Such systems can provide reasonable information concerning physiological and biochemical responses, [12,15] especially if the in vitro-induced response mimics the in vivo plant response exposed to similar stress. [2,9,11,13,14] Such systems can provide reasonable information concerning physiological and biochemical responses, [12,15] especially if the in vitro-induced response mimics the in vivo plant response exposed to similar stress.…”

Olive cuttings survival influences with saline water irrigation

“…[40][41][42][43] In vitro culture systems allow a precise measurement of growth and response to the imposed treatment. [42][43][44][45][46] The exact tolerance of cells to salinity stress is not understood although, 47,48 growth parameters in vitro could reflect the exact tissue response. [40][41][42][43][44][45][46][47][48][49] It has been suggested that whole plant response to salinity is associated with a cellular response, [48][49][50] and the response of cultures to in vitro induced stress mimics the in vivo plant exposed to similar stress.…”

“…[42][43][44][45][46] The exact tolerance of cells to salinity stress is not understood although, 47,48 growth parameters in vitro could reflect the exact tissue response. [40][41][42][43][44][45][46][47][48][49] It has been suggested that whole plant response to salinity is associated with a cellular response, [48][49][50] and the response of cultures to in vitro induced stress mimics the in vivo plant exposed to similar stress. [42][43][44][45][46][47][48][49] There is still a controversial question in the essence of plant cell, callus, or whole plant response to salinity in either way.…”

“…[40][41][42][43][44][45][46][47][48][49] It has been suggested that whole plant response to salinity is associated with a cellular response, [48][49][50] and the response of cultures to in vitro induced stress mimics the in vivo plant exposed to similar stress. [42][43][44][45][46][47][48][49] There is still a controversial question in the essence of plant cell, callus, or whole plant response to salinity in either way. 49,50 Osmotic has been modified as a technology to tolerate or adapt to ambient conditions in many plants.…”