Direct Analysis of Triacylglycerols from Crude Lipid Mixtures by Gold Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry

Son, Jeongjin; Lee, Gwang Bin; Cha, Sangwon

doi:10.1007/s13361-014-0844-9

Cited by 35 publications

(20 citation statements)

References 16 publications

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“…Other lipids (e.g., phospholipids, sphingomyelin, fatty acids, etc.) also consistently formed silver‐analyte adducts, and some species further fragmented in experiments by Muller et al Sputtered gold has been utilized for ionization of cholesterol, but this is only the second report of in solution AuNP sample preparation, giving an opportunity to further probe fundamental ionization effects . It has now been shown that 20‐nm AgNPs, 2‐nm AuNPs, and 5‐nm AuNPs effectively ionize cholesterol.…”

Section: Resultsmentioning

confidence: 99%

Gold nanoparticles for enhanced ionization and fragmentation of biomolecules using LDI‐MS

Sacks

Stumpo

2018

J Mass Spectrom

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New applications for gold nanoparticles (AuNPs) in laser desorption ionization mass spectrometry are presented here. This work expands on previous biomolecule studies and introduces carbohydrates, steroids, bile acids, and other small molecules as a focus. Broad trends in ionization are observed, and specifically of interest are new species that have not previously been reported from AuNPs (e.g., [M + Au] ). Interesting fragmentation effects have been observed for diphenhydramine, including similarity to electron impact mass spectra and possible radical driven reactions, providing insight into the mechanism of ionization when using AuNPs.

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Section: Resultsmentioning

confidence: 99%

Gold nanoparticles for enhanced ionization and fragmentation of biomolecules using LDI‐MS

Sacks

Stumpo

2018

J Mass Spectrom

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“…Lipid assignment for mass peaks obtained by NP-LDI, MALDI, and high-energy Ar cluster SIMS in the mouse brain in positive ion mode (Table 1) and peak assignments for NP-LDI and Ar GCIB SIMS spectra in negative ion mode (Table 2) [26, 40, 48, 66]. The peaks that appear in each MSI technique are weighted by (+).…”

Section: Resultsmentioning

confidence: 99%

Intact lipid imaging of mouse brain samples: MALDI, nanoparticle-laser desorption ionization, and 40 keV argon cluster secondary ion mass spectrometry

et al. 2016

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We have investigated the capability of nanoparticle-assisted laser desorption ionization mass spectrometry (NP-LDI MS), matrix-assisted laser desorption ionization (MALDI) MS, and gas cluster ion beam secondary ion mass spectrometry (GCIB SIMS) to provide maximum information available in lipid analysis and imaging of mouse brain tissue. The use of Au nanoparticles deposited as a matrix for NP-LDI MS is compared to MALDI and SIMS analysis of mouse brain tissue and allows selective detection and imaging of groups of lipid molecular ion species localizing in the white matter differently from those observed using conventional MALDI with improved imaging potential. We demonstrate that high-energy (40 keV) GCIB SIMS can act as a semi-soft ionization method to extend the useful mass range of SIMS imaging to analyze and image intact lipids in biological samples, closing the gap between conventional SIMS and MALDI techniques. The GCIB SIMS allowed the detection of more intact lipid compounds in the mouse brain compared to MALDI with regular organic matrices. The 40 keV GCIB SIMS also produced peaks observed in the NP-LDI analysis, and these peaks were strongly enhanced in intensity by exposure of the sample to trifluororacetic acid (TFA) vapor prior to analysis. These MS techniques for imaging of different types of lipids create a potential overlap and cross point that can enhance the information for imaging lipids in biological tissue sections.Graphical abstractSchematic of mass spectral imaging of a mouse brain tissue using GCIB-SIMS and MALDI techniquesElectronic supplementary materialThe online version of this article (doi:10.1007/s00216-016-9812-5) contains supplementary material, which is available to authorized users.

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“…Another positive effect is that the spatial resolution depends only by instrumental specification like laser spot diameter and not by the crystallization of the matrix16. Metallic NPs have been largely employed in MALDI experiments1718192021 and in particular, TiO2 nanoparticles have been used for distribution studies of small endogenous molecules2223.…”

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confidence: 99%

3D Mass Spectrometry Imaging Reveals a Very Heterogeneous Drug Distribution in Tumors

Giordano

Morosi

Veglianese

et al. 2016

Sci Rep

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Mass Spectrometry Imaging (MSI) is a widespread technique used to qualitatively describe in two dimensions the distribution of endogenous or exogenous compounds within tissue sections. Absolute quantification of drugs using MSI is a recent challenge that just in the last years has started to be addressed. Starting from a two dimensional MSI protocol, we developed a three-dimensional pipeline to study drug penetration in tumors and to develop a new drug quantification method by MALDI MSI. Paclitaxel distribution and concentration in different tumors were measured in a 3D model of Malignant Pleural Mesothelioma (MPM), which is known to be a very heterogeneous neoplasm, highly resistant to different drugs. The 3D computational reconstruction allows an accurate description of tumor PTX penetration, adding information about the heterogeneity of tumor drug distribution due to the complex microenvironment. The use of an internal standard, homogenously sprayed on tissue slices, ensures quantitative results that are similar to those obtained using HPLC. The 3D model gives important information about the drug concentration in different tumor sub-volumes and shows that the great part of each tumor is not reached by the drug, suggesting the concept of pseudo-resistance as a further explanation for ineffective therapies and tumors relapse.

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Direct Analysis of Triacylglycerols from Crude Lipid Mixtures by Gold Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry

Cited by 35 publications

References 16 publications

Gold nanoparticles for enhanced ionization and fragmentation of biomolecules using LDI‐MS

Gold nanoparticles for enhanced ionization and fragmentation of biomolecules using LDI‐MS

Intact lipid imaging of mouse brain samples: MALDI, nanoparticle-laser desorption ionization, and 40 keV argon cluster secondary ion mass spectrometry

3D Mass Spectrometry Imaging Reveals a Very Heterogeneous Drug Distribution in Tumors

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