Direct and indirect antimicrobial effects and antioxidant activity of Cordia gilletii De Wild (Boraginaceae)

Okusa, Philippe N.; Penge, O; Devleeschouwer, Michel; Duez, Pierre

doi:10.1016/j.jep.2007.04.003

Cited by 68 publications

(69 citation statements)

References 14 publications

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“…For bacteria, the stock cultures were subcultured in nutrient broth for incubation at 37°C and for fungi the stock cultures were cultured in Potato dextrose broth at 28°C prior to each antimicrobial testing. The 24 hr culture was stirred with 0.9% saline to achieve 0.5 Mc farland (10 8 cells /ml for bacteria and 10 6 for fungi) (Okusa et al, 2007) .Ciproflaxin and Nystatin were used as reference antbiotics (RA) respectively for bacteria and fungi.…”

Section: Agric Biol J N Am 2010 1(6): 1249-1252mentioning

confidence: 99%

Antimicrobial efficacy of extracts from Alpinia purpurata (Vieill.) K.Schum. against human pathogenic bacteria and fungi

Kochuthressia¹,

Britto²,

M.O.Jaseentha³

et al. 2010

ABJNA

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The antimicrobial activity of three solvent extracts (ethanol, petroleum ether & chloroform) of three different plant parts of Alpinia purpurata (leaves, roots and rhizomes) was studied in vitro against six reference strains of bacteria and four pathogenic fungi using disc diffusion assay. Ethanolic extracts of rhizomes showed a wide spectrum of activity against all tested bacteria, but no notable activity against any fungi except Candida albicans. Highest inhibition zone of (14.4 ± 0.4 mm) was recorded for the ethanolic extracts of rhizomes against Enterobacter aerogens. Petroleum ether and chloroform extracts did not show any highlighting results. The antimicrobial potential of rhizomes of A. purpurata is well substantiated than any other parts tested.

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Section: Agric Biol J N Am 2010 1(6): 1249-1252mentioning

confidence: 99%

Antimicrobial efficacy of extracts from Alpinia purpurata (Vieill.) K.Schum. against human pathogenic bacteria and fungi

Kochuthressia¹,

Britto²,

M.O.Jaseentha³

et al. 2010

ABJNA

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“…Anti-Infective Activities of the D. Trichocarpa Bark Extract On P. aeruginosa PAO1, the MIC and MBC of the D. trichocarpa bark (DTB) n-hexane extract were higher than 4000 µg/ml, indicating an extremely weak potential as a direct antibiotic [11]; by comparison, the MIC and MBC for tobramycin [antibiotic generally used to treat P. aeruginosa infection and particularly pulmonary infection in patients with cystic fibrosis [21] were 1 µg/ml and 2 µg/ml, respectively (data not show).…”

Section: Resultsmentioning

confidence: 99%

“…All inhibited growth culture were then sub-cultured onto LB agar plate and incubated at 37°C for 24 h to determine the MBC which was defined as the lowest concentration that yielded negative sub-cultures [11]. Tobramycin was used as positive control.…”

Section: Antibacterial Assaymentioning

confidence: 99%

An Active Fraction from DalbergiaTrichocarpa Baker Disrupts the Formation and Maintenance of Biofilms in Pseudomonas Aeruginosa PAO1

Rasamiravaka¹,

Labtani²,

Mol³

et al. 2016

International Advanced Research Journal in Science, Engineering

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Abstract:The bark of Dalbergiatrichocapra Baker is traditionally used in Madagascar as an anti-infective remedy. Beyond the recently known anti-quorum sensing (QS) properties of the D. trichocarpabark n-hexane extract, QSindependent anti-infective activities have been also detected. Indeed, chromatographic fractionation allowed the elution of fraction F1 that affects neither bacterial growth nor the expression of QS-related genes (lasB and rhlA) but significantly reduces the formation of biofilm (55.8 ± 2.3%, as compared to control conditions). Moreover,F1 is able to disrupt the structure of one-day old preformed biofilms, which consequently increases the effectiveness of an antibiotic, levofloxacin, on biofilm-encapsulated bacteria (dead bacteria in presence of levofloxacin-F1 were two-fold higher compared to levofloxacin alone). This F1-triggered disruption of biofilm formation is presumably due to an induced reduction in flagellar-dependent motilities (swimming and swarming) as well as in exopolysaccharides production. The inhibitory effect on biofilm appears reversible as the biofilm formation resumes when F1 is discarded from the culture medium. This interesting non-bactericidal mechanism of action may justify the traditional uses of D. trichocarpa in Malagasy medicine. Further work aims at identifying the compound(s) responsible for this biofilm disruption.

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“…The antioxidant activity was determined according to the method by Okusa et al, (2007). Ninety-six-well ELISA plates were filled with extract concentrations ranging from 1-100 µg/mL, HPLC grade methanol served as a blank sample, and a DPPH solution (100 µM) served as a control.…”

Section: Antioxidant Activity (Dpph Free Radical Scavenging)mentioning

confidence: 99%

Antimicrobial activity of the fiber produced by “pochote” <i>Ceiba aesculifolia</i> subsp. <i>parvifolia</i>

Franco¹,

Jiménez-Estrada²,

Hernández-Hernández³

et al. 2016

Afr. J. Trad. Compl. Alt. Med.

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Background: The cotton-like fiber from the fruit of Pochote (Ceiba aesculifolia subsp. parvifolia) can be applied to wounds for healing purposes. As microorganisms can infect wounds and hamper the wound healing process, the aim of this study was to determine the antimicrobial activity and chemical composition of the methanolic extract of Pochote fiber. Materials and Methods: The methanolic extract was tested against bacteria and fungi. For bacteria, the Kirby-Baüer disc diffusion and broth dilution methods were employed to determine the MIC and MBC. In addition, bactericidal kinetic curves were generated. The antifungal activity was determined by the radial diffusion method. The antioxidant activity, total phenolic content, and the flavonoid content were determined. Bioassay guided fractionation was also performed. Results: The methanolic extract showed activity against Enterococcus faecalis, Staphylococcus aureus and Vibrio cholerae (cc). The tested V. cholerae strains were the most sensitive strains, and exhibited a clear CFU size reduction from the bactericidal kinetic curves. The methanolic extract had activity against T. mentagrophytes and R. lilacina. The antioxidant activity (SC 50 = 36.42 μg/mL) was related to the total phenolic (74.4 mg eAG/g) and flavonoid content (21.982 mg (eQ)/g). The bioassay guided fractionation results suggested that the antimicrobial properties of the extract may act through synergism because the total extract had higher activity against bacteria compared to the collected fractions. Conclusion: This study scientifically validates the application of the fruit fiber from Pochote as a part of a traditional medicine approach to alleviate infections caused by bacteria and fungi.

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Direct and indirect antimicrobial effects and antioxidant activity of Cordia gilletii De Wild (Boraginaceae)

Cited by 68 publications

References 14 publications

Antimicrobial efficacy of extracts from Alpinia purpurata (Vieill.) K.Schum. against human pathogenic bacteria and fungi

Antimicrobial efficacy of extracts from Alpinia purpurata (Vieill.) K.Schum. against human pathogenic bacteria and fungi

An Active Fraction from DalbergiaTrichocarpa Baker Disrupts the Formation and Maintenance of Biofilms in Pseudomonas Aeruginosa PAO1

Antimicrobial activity of the fiber produced by “pochote” <i>Ceiba aesculifolia</i> subsp. <i>parvifolia</i>

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