Direct and indirect regeneration of plants from internodal and leaf expiants ofHypencum bupleuroides gris

Abstract

Species of the genus Hypericum are of considerable interest worldwide because of their medicinal properties. In-vitro culture is a useful tool for both multiplication of the genus and studying its economically important secondary metabolites. Here, we present an effective in-vitro propagation method for H. bupleuroides. Leaf and internodal explants excised from 9-week-old, in vitro-germinated seedlings were cultured on a Murashige and Skoog (MS) medium supplemented with benzyladenine (BA; 1.0 or 0.1 mg L -1) and 2,4-dichlorophenoxyacetic acid (2,4-D; 1.0 or 0.1 mg IL 1). Depending on the BA and 2,4-D combination used, these cultures produced adventitious shoot buds directly on the surfaces of both types of explants as well as excessive calli. Numerous shoots were obtained when the calli from both explant types were cultured on an MS medium supplemented with 2 mg L 1 BA. Internodal explants were more responsive than leaf tissues to direct and indirect plant regeneration. After shoots that regenerated from either the calli or the explant surface were excised, rooting was best on an MS medium lacking any growth hormones. These rooted plants were then acclimatized under greenhouse conditions, and 90% of regenerants had survived. Ours is the first report of in-vitro plant regeneration from H. bupleuroides.

Callus induction, high frequency shoot organogenesis and assessment of clonal fidelity in Torenia bicolor Dalzell.

“…Therefore, only the internodal explants were used for the present study. Similar results on superiority of intermodal explants on callus induction and shoot organogenesis have been reported in other systems like Hypencum bupleuroides (Cirak et al, 2007) and Rosa hybrid (Hsia and Korban, 1996). The optimum plant growth regulator combination for callus induction was 4.0 M 2, 4-D and 0.5 M Kn.…”

Hypericum adenotrichum SPACH‘UN KALLUS İNDÜKSİYONU VE ADVENTİF SÜRGÜN REJENERASYONU

“…The highest number of shoots was obtained from nodal explants in the presence of BA and NAA in Hypericum foliosum [30]. In another study, leaf and internodium explants of Hypericum bupleuroides were used and direct shoots were developed in medium containing BA and 2,4 D [31].…”

In vitro propagation, lactucin quantification, and antibacterial activity of Indian lettuce (Lactuca indica L.)

“…Vegetative propagation is also possible, but limited. These are the factors limiting the sustainability and large-scale cultivation of this species; however, these obstacles could be solved through the use of a tissue-culture technique, which is used for mass propagation, disease-free plant production, genetic improvement, and analyzing secondary metabolites for pharmaceutical applications (Pink 1992;Ayan and KevseroĞlu 2007;Georgiev et al 2011;Al Khateeb et al 2012;Salgotra and Gupta, 2015). Also, this method provides a continuous supply of potential resources without any seasonal and environmental limitations, which ensures their practical application on a commercial scale (Kumari et al 2016).…”