Currently, in Portugal, there is a lack of validated scales to measure depression stigma; therefore, validation of the Depression Stigma Scale (DSS) is an essential step to the depression stigma research in Portugal. The sample was collected as part of the OSPI program: Optimising suicide prevention programs and their implementation in Europe, specifically within the application in Portugal, and 1693 participants were included. Floor-ceiling effects and response ranges were analysed, and we calculated Cronbach alphas, conducted a Principal Component Analysis, and Confirmatory Analysis. Construct validity was tested with two well documented hypotheses, using data on gender and depression symptoms. The sample was well comparable with the Portuguese general population, indicating its representativeness. A three-factor structure was identified in each subscale (personal and perceived stigma): weak-not-sick, discrimination, and, dangerous/unpredictable. The Cronbach alphas were satisfactory and construct validity was confirmed. The scale showed good psychometric properties in our sample. More research is needed to test the convergent validity.