Direct assay of <i>Aplysia</i> tissues and cells with laser desorption/ionization mass spectrometry on porous silicon

Kruse, Rebecca A.; Rubakhin, Stanislav S.; Romanova, Elena V.; Bohn, Paul W.; Sweedler, Jonathan V.

doi:10.1002/jms.237

Cited by 72 publications

(66 citation statements)

References 38 publications

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“…16,18 In addition, the sample preparation is minimal, and samples can be stored on DIOS chips for later analysis. DIOS-MS has been utilized in the study of peptides, 19 proteins 20 and small drug molecules. 21,22 MALDI and DIOS have most often been performed in vacuum.…”

Section: Introductionmentioning

confidence: 99%

Analysis of amphetamines and fentanyls by atmospheric pressure desorption/ionization on silicon mass spectrometry and matrix‐assisted laser desorption/ionization mass spectrometry and its application to forensic analysis of drug seizures

Pihlainen

Grigoras²,

Franssila³

et al. 2005

J. Mass Spectrom.

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The suitability of atmospheric pressure desorption/ionization on silicon mass spectrometry (AP-DIOS-MS) and matrix-assisted laser desorption ionization mass spectrometry (AP-MALDI-MS) for the identification of amphetamines and fentanyls in forensic samples was studied. With both ionization techniques, the mass spectra recorded showed abundant protonated molecules, and the background did not disturb the analysis. The use of tandem mass spectrometry (MS/MS) allowed unambiguous identification of the amphetamines and fentanyls. AP-DIOS-MS/MS and AP-MALDI-MS/MS were also successfully applied to the identification of authentic compounds from drug seizures. Common diluents and tablet materials did not disturb the analysis and compounds were unequivocally identified. The limits of detection (LODs) for amphetamines and fentanyls with AP-DIOS-MS/MS were 1-3 pmol, indicating excellent sensitivity of the method. The LODs with AP-MALDI-MS/MS were about 5-10 times higher.

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Section: Introductionmentioning

confidence: 99%

Analysis of amphetamines and fentanyls by atmospheric pressure desorption/ionization on silicon mass spectrometry and matrix‐assisted laser desorption/ionization mass spectrometry and its application to forensic analysis of drug seizures

Pihlainen

Grigoras²,

Franssila³

et al. 2005

J. Mass Spectrom.

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“…For example, neuronal somata are often quite large (up to a millimeter in diameter in the mollusc Aplysia), neurons can be reliably identified and repeatedly studied in different experimental contexts, neural functions are frequently segregated in separate ganglia or regions of ganglia, and neural tissues can be readily and quickly prepared for study in a MALDI Imaging instrument Li et al, 1998Li et al, , 1999Li et al, , 2000bPainter et al, 1998;Rubakhin et al, 1999Rubakhin et al, , 2000Sweedler et al, 2000Sweedler et al, , 2002Furukawa et al, 2001;Kruse et al, 2001;Schein et al, 2001). …”

Section: Invertebrate Nervous System Studies Using Msimentioning

confidence: 97%

Molecular MALDI imaging: An emerging technology for neuroscience studies

Wisztorski

Croix

Macagno

et al. 2008

Developmental Neurobiology

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Mass spectrometry (MS) has become an essential tool for the detection, identification, and characterization of the molecular components of biological processes, such as those responsible for the dynamic properties of the nervous system. Generally, the application of these powerful techniques requires the destruction of the specimen under study, but recent technological advances have made it possible to apply the matrix-assisted laser desorption/ionization (MALDI) MS technique directly to tissue sections. The major advantage of direct MALDI analysis is that it enables the acquisition of local molecular expression profiles, while maintaining the topographic integrity of the tissue and avoiding time-consuming extraction, purification, and separation steps, which have the potential for introducing artifacts. With automation and the ability to display complex spectral data using imaging software, it is now possible to create multiple 2D maps of selected biomolecules in register with tissue sections, a method now known as MALDI Imaging, or MSI (for Mass Spectrometry Imaging). This creates, for example, an opportunity to correlate functional states, determined a priori with live recording or imaging, with the corresponding molecular maps obtained at the time the tissue is frozen and analyzed with MSI. We review the increasing application of MALDI Imaging to the analysis of molecular distributions of proteins and peptides in nervous tissues of both vertebrates and invertebrates, focusing in particular on recent studies of neurodegenerative diseases and early efforts to implement assays of neuronal development.

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“…Therefore, in DIOS, the detectable mass range of small biomolecules, pharmaceutical compounds, amino acids, and oligopeptides can be extended to below m/z 500. [7][8][9] More recently, several other types of nanostructured substrates for organic-matrix-free LDI-MS have been reported. In particular, silicon-based nanomaterials, including nanostructured silicon films, [10] silicon nanowires, [11,12] silicon nanocavity arrays, [13,14] silicon microcolumn arrays, [15] and amorphous silicon, [16] have been extensively studied, and a new technique known as nanostructure-initiator mass spectrometry (NIMS) has recently been proposed.…”

Section: Introductionmentioning

confidence: 99%

Platinum Nanoflowers on Scratched Silicon by Galvanic Displacement for an Effective SALDI Substrate

Kawasaki

Yao

Suganuma

et al. 2010

Chemistry A European J

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We report a new and facile method for synthesizing 3D platinum nanoflowers (Pt Nfs) on a scratched silicon substrate by electroless galvanic displacement and discuss the applications of the Pt Nfs in surface-assisted laser desorption/ionization-mass spectrometry (SALDI-MS). Surface scratching of n-type silicon is essential to induce Pt Nf growth on a silicon substrate (to obtain a Pt Nf silicon hybrid plate) by the galvanic displacement reaction. The Pt Nf silicon hybrid plate showed excellent SALDI activity in terms of the efficient generation of protonated molecular ions in the absence of a citrate buffer. We propose that the acidity of the Si-OH moieties on silicon increases because of the electron-withdrawing nature of the Pt Nfs; hence, proton transfer from the Si-OH groups to the analyte molecules is enhanced, and finally, thermal desorption of the analyte ions from the surface occurs. Signal enhancement was observed for protonated molecular ions produced from a titania nanotube array (TNA) substrate on which Pt nanoparticles had been photochemically deposited. Moreover, surface modification of the Pt Nf silicon hybrid plate by perfluorodecyltrichlorosilane (FDTS) (to obtain an FDTS-Pt Nf silicon hybrid plate) was found to facilitate soft SALDI of labile compounds. More interestingly, the FDTS-Pt Nf silicon hybrid plate acts 1) as a high-affinity substrate for phosphopeptides and 2) as a SALDI substrate. The feasibility of using the FDTS-Pt Nf silicon hybrid plate for SALDI-MS has been demonstrated by using a β-casein digest and various analytes, including small molecules, peptides, phosphopeptides, phospholipids, carbohydrates, and synthetic polymers. The hybridization of Pt Nfs with a scratched silicon substrate has been found to be important for achieving excellent SALDI activity.

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Direct assay of Aplysia tissues and cells with laser desorption/ionization mass spectrometry on porous silicon

Cited by 72 publications

References 38 publications

Analysis of amphetamines and fentanyls by atmospheric pressure desorption/ionization on silicon mass spectrometry and matrix‐assisted laser desorption/ionization mass spectrometry and its application to forensic analysis of drug seizures

Analysis of amphetamines and fentanyls by atmospheric pressure desorption/ionization on silicon mass spectrometry and matrix‐assisted laser desorption/ionization mass spectrometry and its application to forensic analysis of drug seizures

Molecular MALDI imaging: An emerging technology for neuroscience studies

Platinum Nanoflowers on Scratched Silicon by Galvanic Displacement for an Effective SALDI Substrate

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