Direct bioethanol production from wheat straw using xylose/glucose co-fermentation by co-culture of two recombinant yeasts

Zhang, Yuanyuan; Wang, Caiyun; Wang, Lulu; Yang, Ruoxin; Hou, Peilei; Liu, Junhong

doi:10.1007/s10295-016-1893-9

Cited by 31 publications

(13 citation statements)

References 41 publications

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“…2 Owing to its availability, wheat straw has been recently considered as an attractive feedstock for bioethanol 2G production. 3 Usually, wheat straw is transformed into bioethanol 2G following a biorefinery approach in which the polysaccharides of wheat straw (i.e. cellulose and hemicellulose) are hydrolyzed in the following sequence of processes: pretreatment, saccharification, fermentation and purification.…”

Section: Introductionmentioning

confidence: 99%

Optimization of ethyl lactate‐based organosolv process for lignin recovery from the solid waste of a wheat straw biorefinery

Valdespino‐Rodríguez

Pérez-Martín

González‐Barajas

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: The organosolv process promotes biomass delignification at relatively high temperatures using a wide variety of organic solvent solutions. Nevertheless, previous studies on organosolv processes have not dealt with the use of ethyl lactate as organic solvent. Thus the aim of the present contribution was to determine and to validate if the use of ethyl lactate has a positive influence on lignin recovery for organosolv processes whose feedstock is the solid waste from a wheat straw-based biorefinery. The effect of operating conditions (temperature and time) on lignin recovery was also studied. RESULTS: A mixed design of experiments was proposed as the strategy to maximize lignin recovery. The mathematical model derived from a multiple linear regression applied to the mixed design results indicates that 190 ∘ C and a non-aqueous solution containing ethyl lactate and ethanol (81.2% w/w ethyl lactate) could provide a maximum lignin recovery of 75.9% w/w. This theoretical maximum was validated. Thus the proposed model adequately describes the behavior of lignin recovery. Moreover, Fourier transform infrared (FTIR) characterization of the resultant lignin suggested that its structure is a function of the organic solvent solution used during the organosolv process.CONCLUSION: The evidence from this study indicates that lignin recovery from the solid waste of a wheat straw-based biorefinery in an organosolv process depends on temperature and ethyl lactate presence in the reaction medium. These findings enhance the understanding of organosolv processes and could be applied if eventual lignin valorization is desired.Corrections added on 24 January 2020, after first online publication: the second occurrence of the term "m residue " in Equation 1 has been changed to "m sample ". Also the term "m sample " has been included in the sentence "In summary, 300.0 ± 10.0 mg of the solid waste was weighed in a pressure tube" under the section "Washings and lignin content quantification in solid waste".

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Section: Introductionmentioning

confidence: 99%

Optimization of ethyl lactate‐based organosolv process for lignin recovery from the solid waste of a wheat straw biorefinery

Valdespino‐Rodríguez

Pérez-Martín

González‐Barajas

et al. 2019

J of Chemical Tech & Biotech

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“… BGL1, EG, CBHI ( T. reesei ). Alkaline peroxide pretreated wheat straw 24 0.36 [ 139 ] Secretion. Industrial-derived host BGL1 ( S. fibuligera ); CBHI ( Talaromyces emersonii ).…”

Section: Consolidated Bioprocessingmentioning

confidence: 99%

“…With this, Gong and collaborators produced 4.6 g/L of ethanol from 20 g/L of carboxymethyl cellulose (CMC) by secreting BGL1 and EG from Trichoderma viride in an industrial host [ 138 ]. Also, through secretion of BGL1, EG, and CBHI from Trichoderma reesei , 24 g/L of ethanol were obtained from alkaline peroxide-pretreated wheat straw [ 139 ]. In a different approach, the expression of a single-enzyme-system-three-cellulase gene isolated from Ampullaria gigas Spix, with endo-beta-1,4-glucanase, exo-beta-1,4-glucanase, and xylanase activities, resulted in the production of 8.1 g/L of ethanol from 80 g/L of rice straw and 20 g/L of wheat bran [ 140 ].…”

Section: Consolidated Bioprocessingmentioning

confidence: 99%

Engineered Saccharomyces cerevisiae for lignocellulosic valorization: a review and perspectives on bioethanol production

et al. 2020

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The biorefinery concept, consisting in using renewable biomass with economical and energy goals, appeared in response to the ongoing exhaustion of fossil reserves. Bioethanol is the most prominent biofuel and has been considered one of the top chemicals to be obtained from biomass. Saccharomyces cerevisiae, the preferred microorganism for ethanol production, has been the target of extensive genetic modifications to improve the production of this alcohol from renewable biomasses. Additionally, S. cerevisiae strains from harsh industrial environments have been exploited due to their robust traits and improved fermentative capacity. Nevertheless, there is still not an optimized strain capable of turning second generation bioprocesses economically viable. Considering this, and aiming to facilitate and guide the future development of effective S. cerevisiae strains, this work reviews genetic engineering strategies envisioning improvements in 2 nd generation bioethanol production, with special focus in process-related traits, xylose consumption, and consolidated bioprocessing. Altogether, the genetic toolbox described proves S. cerevisiae to be a key microorganism for the establishment of a bioeconomy, not only for the production of lignocellulosic bioethanol, but also having potential as a cell factory platform for overall valorization of renewable biomasses.

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“…Bioethanol production from various carbohydrate-based substrates and wastes such as DFW has been explored by many researchers that have proposed different fermentation strategies via monocultures of bacteria or yeasts [5][6][7][8][9][10] but also co-cultures of yeasts, fungi, and bacteria [11][12][13][14][15]. Unlike other types of biomass, such as agricultural and forestry residues, prunings, olive mill wastes, etc.…”

Section: Introductionmentioning

confidence: 99%

Sustainable Second-Generation Bioethanol Production from Enzymatically Hydrolyzed Domestic Food Waste Using Pichia anomala as Biocatalyst

2020

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In the current study, a domestic food waste containing more than 50% of carbohydrates was assessed as feedstock to produce second-generation bioethanol. Aiming to the maximum exploitation of the carbohydrate fraction of the waste, its hydrolysis via cellulolytic and amylolytic enzymatic blends was investigated and the saccharification efficiency was assessed in each case. Fermentation experiments were performed using the non-conventional yeast Pichia anomala (Wickerhamomyces anomalus) under both separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) modes to evaluate the conversion efficiencies and ethanol yields for different enzymatic loadings. It was shown that the fermentation efficiency of the yeast was not affected by the fermentation mode and was high for all handlings, reaching 83%, whereas the enzymatic blend containing the highest amount of both cellulolytic and amylolytic enzymes led to almost complete liquefaction of the waste, resulting also in ethanol yields reaching 141.06 ± 6.81 g ethanol/kg waste (0.40 ± 0.03 g ethanol/g consumed carbohydrates). In the sequel, a scale-up fermentation experiment was performed with the highest loading of enzymes in SHF mode, from which the maximum specific growth rate, μmax, and the biomass yield, Yx/s, of the yeast from the hydrolyzed waste were estimated. The ethanol yields that were achieved were similar to those of the respective small scale experiments reaching 138.67 ± 5.69 g ethanol/kg waste (0.40 ± 0.01 g ethanol/g consumed carbohydrates).

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Direct bioethanol production from wheat straw using xylose/glucose co-fermentation by co-culture of two recombinant yeasts

Cited by 31 publications

References 41 publications

Optimization of ethyl lactate‐based organosolv process for lignin recovery from the solid waste of a wheat straw biorefinery

Optimization of ethyl lactate‐based organosolv process for lignin recovery from the solid waste of a wheat straw biorefinery

Engineered Saccharomyces cerevisiae for lignocellulosic valorization: a review and perspectives on bioethanol production

Sustainable Second-Generation Bioethanol Production from Enzymatically Hydrolyzed Domestic Food Waste Using Pichia anomala as Biocatalyst

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