Direct CdTe Quantum‐Dot‐Based Fluorescence Imaging of Human Serum Proteins

Na, Na; Liu, Lu; Taes, Youri; Zhang, Canli; Huang, Biao; Liu, Yueli; Ma, Lin; Ouyang, Jin

doi:10.1002/smll.201000684

Cited by 25 publications

(21 citation statements)

References 23 publications

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“…3, the curves show abundant peaks with time, which is similar to the migration of proteins during electrophoresis. 31 In detail, no signal was obtained until 2 min for all the three charge states. Subsequently, the signals gradually increased and reached the strongest value at about 4 min, and then began to decrease and reached the lowest value at approximately 6.5 min.…”

Section: Migration Of Proteins During Detectionmentioning

confidence: 99%

Direct analysis of in-gel proteins by carbon nanotubes-modified paper spray ambient mass spectrometry

Han

Yang

Ouyang

et al. 2015

Analyst

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The in situ and direct extraction, desorption and ionization of in-gel intact proteins after electrophoresis has been achieved by carbon nanotubes (CNTs)-modified paper spray mass spectrometry at ambient conditions. Characteristics of CNTs (including larger surface area, smaller pore diameter and enhanced conductivity) were endowed to the porous filter paper substrate by uniformly dispersing the CNTs on the filter paper. Upon applying electric potential to the CNTs-modified paper, the in-gel proteins were extracted from the gel and subsequently migrated to the tip of the filter paper by electrophoresis-like behavior for paper spray ionization, which was monitored by extracted ion chronograms. The characterizations of modified filter papers and CNTs nanoparticles further confirmed the role of CNTs in in-gel protein extraction, protein migration as well as spray ionization at the paper tip. Under optimized conditions, a mixture of cytochrome c, lysozyme and myoglobin was successfully separated by native electrophoresis and subsequently analysed by the present method, showing a limit of detection of 10 ng per gel band. The present strategy offers a new pathway for the direct detection of in-gel intact proteins at ambient conditions without any pre-treatment (e.g. digestion, chemical extraction and desalting), which exhibits potential applications in top-down proteomics.

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Section: Migration Of Proteins During Detectionmentioning

confidence: 99%

Direct analysis of in-gel proteins by carbon nanotubes-modified paper spray ambient mass spectrometry

Han

Yang

Ouyang

et al. 2015

Analyst

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“…Non-covalent interactions between QDs and proteins/ peptides have been investigated [22,36] and successfully applied in colorimetric analysis of proteins [37], intracellular delivery [5], live cell imaging [1] etc., where the electrostatic and coordinate interactions play important roles in the formation of QD-protein/peptide complex. As the TomSys has an isoelectric point of 9.70 and several positive charge centers of lysines and arginine, it could be expected that there are certain electrostatic interactions between the TomSys and 3-MPA capped CdTe/CdS/ZnS QDs at a moderate pH.…”

Section: In-capillary Non-covalent Labeling Of Tomsys With Qdsmentioning

confidence: 99%

In‐capillary non‐covalent labeling and determination of tomato systemin with quantum dots in capillary electrophoresis with laser‐induced fluorescence detection

Bai¹,

Du²,

Yang³

et al. 2011

J of Separation Science

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Quantum dots (QDs), with their superior size-dependent fluorescence properties, have been employed as non-covalent fluorescent labels for the determination of tomato systemin (TomSys) by capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection. The optimum conditions of in-capillary labeling and CE separation were investigated in detail, and complete separation of QDs-labeled TomSys from free QDs labels was achieved. Satisfactory results were obtained in terms of linearity (R(2)=0.998), sensitivity (limit of detection, 66 fmol) and repeatability (run-to-run RSDs of migration time and peak area, 0.9 and 4.6%, respectively; day-to-day RSDs of migration time and peak area, 3.1 and 11.9%, respectively). The established CE-LIF method was later applied in the detection of TomSys spiked in the sample of tomato leaves, which showed the applicability of the proposed method in the analysis of the target plant peptide hormone in the complex matrix.

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“…Fluorescent dye staining also has some weak points, for instance, narrow excitation spectra, and the requirement for excitation by lights of a specific wavelength varying between particular dyes. [14] We have developed nanosized fluorescent probes because they have the advantage of higher sensitivity. Our previous work has shown that Ag nanoclusters can be applied in fluorescent imaging of human serum proteins after native polyacrylamide gel electrophoresis.…”

Section: Introductionmentioning

confidence: 99%

“…However, traditional and prevalent methods, such as CBB staining, meet with drawbacks of low sensitivity and tedious staining procedures. Fluorescent dye staining also has some weak points, for instance, narrow excitation spectra, and the requirement for excitation by lights of a specific wavelength varying between particular dyes 14. We have developed nanosized fluorescent probes because they have the advantage of higher sensitivity.…”

Section: Introductionmentioning

confidence: 99%

The Application of Amine‐Terminated Silicon Quantum Dots on the Imaging of Human Serum Proteins after Polyacrylamide Gel Electrophoresis (PAGE)

Huang

et al. 2012

Chemistry A European J

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Novel amine-terminated silicon (Si) quantum dots (QDs) were synthesized and applied for the detection of human serum proteins on gels directly after polyacrylamide gel electrophoresis (PAGE). The diameter of these stable amine-terminated Si QDs was in the range of 0.5-2.0 nm. In this study, the fluorescent imaging conditions, such as the buffer solution, pH value, buffer concentration and quantity of Si QDs, were optimized and the possible mechanisms of Si QDs-protein interaction were analyzed. The mode of Si QDs and human serum albumin association was found to occur by hydrogen bond interactions; this was probably attributed to the interaction between the amino group of amine-terminated Si QDs and the carboxyl group of proteins. Meanwhile, human serum proteins separated by native 1D and native 2D electrophoresis were detected by Si QD-based fluorescent imaging. Some proteins, such as isoform 1 of α-1-antitrypsin, complement C3 (Fragment) and hemopexin, which were identified by mass spectrometry (MS), were easily detected by using Si QDs, but not with CBB-R250 staining. The Si QDs-based fluorescent imaging technique with high resolution is a sensitive and dependable method for direct detection of human serum proteins, and has enormous potential in clinical diagnosis.

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Direct CdTe Quantum‐Dot‐Based Fluorescence Imaging of Human Serum Proteins

Cited by 25 publications

References 23 publications

Direct analysis of in-gel proteins by carbon nanotubes-modified paper spray ambient mass spectrometry

Direct analysis of in-gel proteins by carbon nanotubes-modified paper spray ambient mass spectrometry

In‐capillary non‐covalent labeling and determination of tomato systemin with quantum dots in capillary electrophoresis with laser‐induced fluorescence detection

The Application of Amine‐Terminated Silicon Quantum Dots on the Imaging of Human Serum Proteins after Polyacrylamide Gel Electrophoresis (PAGE)

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