Direct cell imprint lithography in superconductive carbon black polymer composites: process optimization, characterization and
in vitro
toxicity analysis
Abstract:Cell imprint lithography (CIL) or cell replication plays a vital role in fields like biomimetic smart culture substrates, bone tissue engineering, cell guiding, cell adhesion, tissue engineering, cell microenvironments, tissue microenvironments, cell research, drug delivery, diagnostics, therapeutics and many other applications. Herein we report a new formulation of superconductive carbon black photopolymer composite and its characterization towards a CIL process technique. In this article, we demonstrated an … Show more
“…This process is characteristic of polyesters; during the heating, some regions of the polymeric chains acquire mobility, which allows the organization of non-crystallized segments. 13 As for the effects of the printing conditions, it is possible to observe that the increase in speed and printing temperature favored the increase in T g , T m , and crystallinity degree of the samples. This result corroborates the XRD findings, indicating that these conditions favor the orientation of the filament during the printing process.Figure 5.DSC curves of the Tg region (a) and total run of the third heating (b) of the systems with different printing parameters.…”
Section: Resultsmentioning
confidence: 99%
“…The system cytotoxicity was evaluated in L929 lineage cells (provided from Rio de Janeiro Cell Bank–BCRJ) through neutral red dye analysis, following the methodology described by Narayanamurthy et al (2019) 13 and ISO 10993-5 14…”
3D printing techniques are of great interest in the sector of scaffold development aiming for bone tissue regeneration mainly due to the possibility of customizing the scaffold according to the area of the bone defect to be regenerated. Among the 3D printing techniques, the fused deposition modeling (FDM) stands out as promising because it does not require the use of solvents and toxic components throughout the manufacturing process of the scaffold. In this sense, the present article aims to evaluate the influence of the printing speed and the temperature of the printing head on the properties of poly(lactic acid) scaffolds. Three speeds of the printing head (4600 mm/min, 480 mm/min, and 500 mm/min) and two different extrusion temperatures (200oC and 220oC) were evaluated, maintaining the architecture and all other printing conditions constant. After obtaining the scaffolds, they were characterized by the following techniques: Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), time-domain nuclear magnetic resonance (TD-NMR), compressive modulus, L929 cell viability, and enzymatic degradation. The results obtained showed that the increase in printing temperature and speed was able to influence some properties of the material: increase crystallinity, compressive modulus, thermal resistance, and reduce molecular mobility and enzymatic degradation rate of the scaffolds. These findings are promising and indicate that, by altering only the basic parameters of 3D printing, it is possible to modulate the properties of the scaffolds obtained, to achieve greater crystallinity and a superior compressive modulus.
“…This process is characteristic of polyesters; during the heating, some regions of the polymeric chains acquire mobility, which allows the organization of non-crystallized segments. 13 As for the effects of the printing conditions, it is possible to observe that the increase in speed and printing temperature favored the increase in T g , T m , and crystallinity degree of the samples. This result corroborates the XRD findings, indicating that these conditions favor the orientation of the filament during the printing process.Figure 5.DSC curves of the Tg region (a) and total run of the third heating (b) of the systems with different printing parameters.…”
Section: Resultsmentioning
confidence: 99%
“…The system cytotoxicity was evaluated in L929 lineage cells (provided from Rio de Janeiro Cell Bank–BCRJ) through neutral red dye analysis, following the methodology described by Narayanamurthy et al (2019) 13 and ISO 10993-5 14…”
3D printing techniques are of great interest in the sector of scaffold development aiming for bone tissue regeneration mainly due to the possibility of customizing the scaffold according to the area of the bone defect to be regenerated. Among the 3D printing techniques, the fused deposition modeling (FDM) stands out as promising because it does not require the use of solvents and toxic components throughout the manufacturing process of the scaffold. In this sense, the present article aims to evaluate the influence of the printing speed and the temperature of the printing head on the properties of poly(lactic acid) scaffolds. Three speeds of the printing head (4600 mm/min, 480 mm/min, and 500 mm/min) and two different extrusion temperatures (200oC and 220oC) were evaluated, maintaining the architecture and all other printing conditions constant. After obtaining the scaffolds, they were characterized by the following techniques: Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), time-domain nuclear magnetic resonance (TD-NMR), compressive modulus, L929 cell viability, and enzymatic degradation. The results obtained showed that the increase in printing temperature and speed was able to influence some properties of the material: increase crystallinity, compressive modulus, thermal resistance, and reduce molecular mobility and enzymatic degradation rate of the scaffolds. These findings are promising and indicate that, by altering only the basic parameters of 3D printing, it is possible to modulate the properties of the scaffolds obtained, to achieve greater crystallinity and a superior compressive modulus.
“…The study of the cytotoxicity of the systems was carried out in a L929 cell line Materials Sciences and Applications (provided from Rio de Janeiro Cell Bank-BCRJ) following the methodology of Narayanamurthy et al [13] and the standards of ISO 10993-5 [14]. To this end, we used fibroblasts from the L929 cell line that were cultivated in essential medium supplemented with gentamicin and glutamine in a controlled environment at 37˚C and 5% CO 2 .…”
Section: Cell Viability Of the L929 Fibroblast Lineagementioning
3D printing is a valuable resource that allows flexibility in the production of objects based on a virtual file. When it is combined with nanotechnology, new features can be added to existing materials. Thus, form and function can be associated to achieve a specific goal, such as the development of support structures for cell growth applicable to systems aiding tissue regeneration. Based on this rationale, the present work proposes a system composed of ABS and graphene nanoparticles solubilized in acetone to be 3D impressed using solvent casting technique. Our main goal was to develop a biocompatible and non-degradable material that fully makes use of the design versatility of 3D printing, to enable new practical employments in the future, for example in the medical field. In this study, different characterization techniques were used-such as microscopy, TGA, DSC, and others-to understand the features and properties of the material obtained, as well as the viability of its use and diffusion. Moreover, the artifacts impressed proved to be non-cytotoxic and promoted cellular adhesion to the cellular lineage of fibroblasts L929. In sum, we believe that the technology described in this article has the potential to serve as a basis for the development of future biocompatible materials that take advantage of their three-dimensional design to perform their functions.
“…Controlling surface functionalization is essential for constructing and utilizing composite materials [1–5] . In particular, failure at the interface of the composite materials frequently occurs because of the weak adhesion between two or more materials.…”
Section: Introductionmentioning
confidence: 99%
“…Controlling surface functionalization is essential for constructing and utilizing composite materials. [ 1 , 2 , 3 , 4 , 5 ] In particular, failure at the interface of the composite materials frequently occurs because of the weak adhesion between two or more materials. Acrylate‐based resins are widely used as surface adhesives in composite materials because of their tunable bonding strength, design flexibility, and durability.…”
Marine mussels contain an abundant catechol moiety, 3,4‐dihydroxyphenylalanine (DOPA), in their interfacial foot proteins. DOPA contributes to both surface adhesion and bridging between the surface and overhead proteins (surface priming) by taking advantage of the unique redox properties of catechol. Inspired by the mussel surface priming mechanism, herein we synthesized a series of DOPA‐mimetic analogs – a bifunctional group molecule, consisting of a catechol group and an acrylic group at the opposite ends. The surface primers with differently substituted (−COOH, −CH3) alkyl chains in the middle spacer were synthesized. Time‐dependent oxidation and redox potentials of the surface primers were studied in an oxidizing environment to gain a better understanding of the mussel‘s redox chemistry. The thickness and degree of priming of the surface primers on silicon‐based substrates were analyzed by ellipsometry and UV/Vis absorption spectroscopy. The post‐reactivity of the acrylic groups of the primed layer was first visualized through a reaction with an acrylic group‐reactive dye.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
