Direct cleavage during the first mitosis is a sign of abnormal fertilization in cattle

Suzuki, Ryosuke; Yao, Tatsuma; Okada, Mai; Nagai, Hiroki; Khurchabilig, Atchalalt; Kobayashi, Jun’ichi; Yamagata, Kazuya; Sugimura, Satoshi

doi:10.1016/j.theriogenology.2023.01.028

Cited by 6 publications

(5 citation statements)

References 40 publications

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“…Recently, it has been shown that multi pronuclei and migration error of pronuclei are the primary reasons for direct cleavage in cattle (73). Using TLM we were able to observe a higher probability of direct cleavage in zygotes of cows with combined and inflammatory disease.…”

Section: Discussionmentioning

confidence: 74%

“…Using TLM we were able to observe a higher probability of direct cleavage in zygotes of cows with combined and inflammatory disease. This might be due to the negative effects of intrafollicular inflammatory agents on oocyte spindle assembly, considering that abnormalities in oocyte microtubule positioning lead to failure in pronuclei apposition (73). As direct cleavage was previously associated with embryonic arrest in cattle (41,74), it would be interesting to analyze the pregnancy rate after transfer of embryos from diseased cows produced in this study.…”

Section: Discussionmentioning

confidence: 96%

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Impact of negative energy balance and postpartum diseases during the transition period on oocyte quality and embryonic development in dairy cows

Serbetci,

González-Grajales,

Herrera

et al. 2024

Front. Vet. Sci.

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Transition period is a critical time for dairy cows because a large proportion of clinical and subclinical diseases are observed in the first month after parturition. Occurrence of negative energy balance is associated with depressed immunity and these conditions can affect oocyte quality and further embryonic development. The aim of this study was to assess the effects of negative energy balance-associated disorders on in vitro embryo production (IVP) in dairy cattle. We hypothesized that subclinical metabolic and/or inflammatory disorders have a negative effect on oocyte developmental competence and morphokinetic parameters of the resulting embryos. The study was conducted on 30 lactating Holstein-Friesian cows which were assigned into four groups: healthy (HEAL, n = 6), metabolic disease (META, n = 8), inflammatory disease (INFL, n = 8), or combined metabolic and inflammatory disease (COMB, n = 8). Ovum pick-up (OPU) was performed twice weekly on all cows over a period of four weeks (n = 8 OPU sessions/cow) starting on the fifth week postpartum, and the collected oocytes were subjected to routine IVP. Donor’s health status did not affect the number of oocytes/OPU or the recovery rate (p > 0.05). The number of quality 1 oocytes collected from INFL and COMB cows was lower compared to HEAL cows (p < 0.05). Also, the percentage of quality 1 embryos was reduced in META and COMB compared to HEAL cows (p < 0.05). Cleavage, blastocyst and hatching rates were similar among groups (p > 0.05). Presence of disease did not affect the time required by zygotes to reach specific developmental stages, as recorded by means of time-lapse monitoring. Nevertheless, there was a higher probability of direct cleavage after IVF in oocytes of COMB cows compared to those of HEAL cows (p < 0.05). In conclusion, oocytes and embryos derived from dairy cows diagnosed with subclinical metabolic and/or inflammatory diseases during the transition period showed reduced quality but similar developmental potential and morphokinetics when compared to healthy cows. These results shed light on the consequences of subclinical disease on embryonic development in dairy cows which might be important for embryo transfer programs.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 96%

Impact of negative energy balance and postpartum diseases during the transition period on oocyte quality and embryonic development in dairy cows

Serbetci,

González-Grajales,

Herrera

et al. 2024

Front. Vet. Sci.

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“…As above discussed, direct cleavage acted as the main reasons for fastcleaving embryos [19]. Despite the exact cause of direct cleavage remained unclear, the incidence of direct cleavage increased in zygotes with multi-pronuclei and those showing abnormal pronuclei migration and tripolar or tetrapolar spindle formation [19,46,47]. This probably meant that some of 0PN zygotes actually were multipronuclei zygotes which missed xed-point assessment of fertilization.…”

Section: Discussionmentioning

confidence: 99%

Developmental competence and neonatal outcomes of nonpronuclear zygotes following single vitrified-warmed blastocyst transfers using propensity score matching analysis

Zhu,

Wang,

Cao

et al. 2023

Arch Gynecol Obstet

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Purpose To investigate developmental competence and neonatal outcomes of nonpronuclear (0PN) zygotes following single vitri ed-warmed blastocyst transfers (VBT).Methods The clinical, laboratorial and neonatal data of 996 patients with ≤38 years who underwent blastocyst culture and single VBT were retrospectively analyzed. The pregnancy and neonatal outcomes of VBT were compared between 0PN and 2PN blastocysts using propensity score matching (PSM). Moreover, Day 3 (D3) embryo development and blastocyst formation were compared between 0PN and 2PN zygotes.ResultsThere were no signi cant differences in clinical pregnancy rate (CPR), live birth rate (LBR) and neonatal outcomes of VBT between the 0PN and 2PN blastocysts irrespectively of whether PSM was used. However, early abortion rate (EAR) was higher in blastocysts from 0PN D3 embryos 10 cells (p 0.05) before PSM. Moreover, the early developmental competence of 0PN zygotes was different from that of 2PN zygotes presenting higher percentages of D3 embryos ≤6 cells (p 0.01) and 10 cells (p 0.01), lower available blastocyst formation rate (ABFR) (p 0.01) and good-quality blastocyst formation rate (GBFR) (p 0.01) in D3 embryos with 4-6 cells. ABFR and GBFR increased with cell number when compared among embryos with 4-6 cells, 7-10 cells and 10 cells, irrespectively of 0PN or 2PN embryos.ConclusionThe early developmental competence of 0PN zygotes was different from that of 2PN zygotes, but did not in uence pregnancy and neonatal outcomes following VBT. ABFR and GBFR increased with cell number, irrespectively of 0PN or 2PN embryos.What does this study add to the clinical work?The early developmental competence of 0PN zygotes was different from that of 2PN zygotes, but did not in uence pregnancy and neonatal outcomes following VBT.The rates of available blastocyst and good-quality blastocyst increased with cell number of D3 embryos, irrespectively of 0PN or 2PN embryos.

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“…There could be several reasons for this observation. First, delayed blastulation (time from fading of pronuclei to blastocyst stage) occurs in embryos with multi-pronuclei (PN) in bovine models [ 95 ] and is associated with abnormal fertilization [ 96 ]. Multi-PNs in human zygotes are also associated with high rates of aneuploidy [ 97 , 98 ].…”

Section: Discussionmentioning

confidence: 99%

Machine learning in time-lapse imaging to differentiate embryos from young vs old mice

Yang,

Leynes,

Pawelka

et al. 2024

Biology of Reproduction

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Time-lapse microscopy for embryos is a non-invasive technology used to characterize early embryo development. This study employs time-lapse microscopy and machine learning to elucidate changes in embryonic growth kinetics with maternal aging. We analyzed morphokinetic parameters of embryos from young and aged C57BL6/NJ mice via continuous imaging. Our findings show that aged embryos accelerated through cleavage stages (from 5-cells) to morula compared to younger counterparts, with no significant differences observed in later stages of blastulation. Unsupervised machine learning identified two distinct clusters comprising of embryos from aged or young donors. Moreover, in supervised learning, the XGBoost (extreme gradient boosting) algorithm successfully predicted the age-related phenotype with 0.78 accuracy, 0.81 precision, and 0.83 recall following hyperparameter tuning. These results highlight two main scientific insights: maternal aging affects embryonic development pace, and that AI can differentiate between embryos from aged and young maternal mice by a non-invasive approach. Thus, machine learning can be used to identify morphokinetics phenotypes for further studies. This study has potential for future applications in selecting human embryos for embryo transfer, without or in complement with preimplantation genetic testing.

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Direct cleavage during the first mitosis is a sign of abnormal fertilization in cattle

Cited by 6 publications

References 40 publications

Impact of negative energy balance and postpartum diseases during the transition period on oocyte quality and embryonic development in dairy cows

Impact of negative energy balance and postpartum diseases during the transition period on oocyte quality and embryonic development in dairy cows

Developmental competence and neonatal outcomes of nonpronuclear zygotes following single vitrified-warmed blastocyst transfers using propensity score matching analysis

Machine learning in time-lapse imaging to differentiate embryos from young vs old mice

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