Direct Comparison of Disaccharide Interaction with Lipid Membranes at Reduced Hydrations

Kent, Ben; Hauß, Thomas; Demé, Bruno; Cristiglio, Viviana; Darwish, Tamim A.; Hunt, Taavi; Bryant, Gary; Garvey, Christopher J.

doi:10.1021/acs.langmuir.5b02127

Cited by 23 publications

(26 citation statements)

References 54 publications

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“…Complementary physical methods are continuously improved to investigate interactions between molecules and lipid bilayers (Deleu et al 2014;Kent et al 2015).…”

Section: Methodological Breakthroughsmentioning

confidence: 99%

Factors influencing the membrane fluidity and the impact on production of lactic acid bacteria starters

Fonseca

Pénicaud

Tymczyszyn

et al. 2019

Appl Microbiol Biotechnol

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Production of lactic acid bacteria starters for manufacturing food, probiotic, and chemical products requires the application of successive steps: fermentation, concentration, stabilization, and storage. Despite process optimization, losses of bacterial viability and functional activities are observed after stabilization and storage steps due to cell exposure to environmental stresses (thermal, osmotic, mechanical, and oxidative). Bacterial membrane is the primary target for injury and its damage is highly dependent on its physical properties and lipid organization. Membrane fluidity is a key property for maintaining cell functionality, and depends on lipid composition and cell environment. Extensive evidence has been reported on changes in membrane fatty acyl chains when modifying fermentation conditions. However, a deep characterization of membrane physical properties and their evolution following production processes is scarcely reported. Therefore, the aims of this mini-review are (i) to define the membrane fluidity and the methods used to assess it and (ii) to summarize the effect of environmental conditions on membrane fluidity and the resulting impact on the resistance of lactic acid bacteria to the stabilization processes. This will make it possible to highlight existing gaps of knowledge and opens up novel approaches for future investigations.

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“…Complementary physical methods are continuously improved to investigate interactions between molecules and lipid bilayers (Deleu et al 2014;Kent et al 2015).…”

Section: Methodological Breakthroughsmentioning

confidence: 99%

Factors influencing the membrane fluidity and the impact on production of lactic acid bacteria starters

Fonseca

Pénicaud

Tymczyszyn

et al. 2019

Appl Microbiol Biotechnol

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“…When a desiccated membrane is rehydrated, the transition from the gel phase back to the liquid crystal phase does not occur uniformly across the membrane; some regions remain packed in the gel phase while others spread out in the liquid crystal phase (Fig. 2 d and e) [ 48 – 50 ] . Because of this non-uniform phase transition the membrane cannot expand to accommodate increased water volume during rehydration, ultimately causing transient holes to form in the membrane.…”

Section: Main Textmentioning

confidence: 99%

The biology of tardigrade disordered proteins in extreme stress tolerance

Hesgrove

Boothby

2020

Cell Commun Signal

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Disordered proteins have long been known to help mediate tolerance to different abiotic stresses including freezing, osmotic stress, high temperatures, and desiccation in a diverse set of organisms. Recently, three novel families of intrinsically disordered proteins were identified in tardigrades, microscopic animals capable of surviving a battery of environmental extremes. These three families include the Cytoplasmic-, Secreted-, and Mitochondrial- Abundant Heat Soluble (CAHS, SAHS, and MAHS) proteins, which are collectively termed Tardigrade Disordered Proteins (TDPs). At the level of sequence conservation TDPs are unique to tardigrades, and beyond their high degree of disorder the CAHS, SAHS, and MAHS families do not resemble one another. All three families are either highly expressed constitutively, or significantly enriched in response to desiccation. In vivo, ex vivo, and in vitro experiments indicate functional roles for members of each TDP family in mitigating cellular perturbations induced by various abiotic stresses. What is currently lacking is a comprehensive and holistic understanding of the fundamental mechanisms by which TDPs function, and the properties of TDPs that allow them to function via those mechanisms. A quantitative and systematic approach is needed to identify precisely what cellular damage TDPs work to prevent, what sequence features are important for these functions, and how those sequence features contribute to the underlying mechanisms of protection. Such an approach will inform us not only about these fascinating proteins, but will also provide insights into how the sequence of a disordered protein can dictate its functional, structural, and dynamic properties. Graphical abstract

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“…These two relationships allow us to calculate S and DP from the experimentally observed curvature radii R a and R b provided we know the bending rigidity k and the spontaneous curvature m. It is instructive to rewrite the mechanical tension S as given by eqn (12) in terms of the neck curvature M ab as given by eqn (7) and of the spontaneous tension 37 s 2km 2 (14) which leads to…”

Section: Mechanical Tension and Pressure Difference For Multisphericamentioning

confidence: 99%

“…As a consequence, the sugars experience effectively repulsive interactions with the lipids and are partially excluded from the hydration shells of the membranes. [9][10][11][12][13][14] Other experimental studies provide evidence that the lipids prefer to form hydrogen bonds with the sugar molecules, which then experience effectively attractive interactions towards the membranes. [15][16][17][18][19][20][21] These opposing views have been reconciliated by the proposal that the sugar-lipid interactions depend on the bulk concentration of the sugar, being attractive for low concentrations and repulsive for high concentrations.…”

Section: Introductionmentioning

confidence: 99%