Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Clark, Matthew G.; Brasseale, Kent A.; Gonzalez, Gil A.; Eakins, Gregory; Zhang, Chi

doi:10.3791/63677-v

Cited by 1 publication

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The CARS image contrast is usually mixed with the nonresonant background, whereas the SRS signal is relatively clean unless strong absorption‐induced transient absorption or photothermal effect may occur. Due to the interference of the nonresonant background, raw CARS spectra might show slightly broader peaks with red‐shifted peak centers 101 . Due to the higher order laser intensity dependence of the pump beam, the CARS image gives better spatial resolution compared with SRS under the same imaging condition 101 .…”

Section: Discussionmentioning

confidence: 99%

“…Due to the interference of the nonresonant background, raw CARS spectra might show slightly broader peaks with red‐shifted peak centers 101 . Due to the higher order laser intensity dependence of the pump beam, the CARS image gives better spatial resolution compared with SRS under the same imaging condition 101 . Due to the position‐dependent phase effect, small LDs above the focus usually have positive intensity, and those below the focus might have a shadowing effect showing as dark spots in CARS images 42 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Coherent Raman scattering microscopy of lipid droplets in cells and tissues

Zhang

2023

J Raman Spectroscopy

View full text Add to dashboard Cite

Lipid droplets (LDs) play a key role as the hub for lipid metabolism to maintain cellular metabolic homeostasis. Understanding the functions and changes of LDs in different pathological conditions is crucial for identifying new markers for diagnosis and discovering new targets for treatment. In recent years, coherent Raman scattering (CRS) microscopy has been popularized for the imaging and quantification of LDs in live cells. Compared with spontaneous Raman scattering microscopy, CRS microscopy offers a much higher imaging speed while maintaining similar chemical information. Due to the high lipid density, LDs usually have strong CRS signals and therefore are the most widely studied organelle in the CRS field. In this review, we discuss recent achievements using CRS to study the quantity, distribution, composition, and dynamics of LDs in various systems.

show abstract

Section: Discussionmentioning

confidence: 99%