Direct Comparison of Mice Null for Liver or Intestinal Fatty Acid-binding Proteins Reveals Highly Divergent Phenotypic Responses to High Fat Feeding

Gajda, Angela M.; Zhou, Yin; Agellon, Luis B.; Fried, Susan K.; Kodukula, Sarala; Fortson, Walter M.; Patel, Khamoshi; Storch, Judith

doi:10.1074/jbc.m113.501676

Cited by 48 publications

(85 citation statements)

References 82 publications

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“…This result was reproducible by others using the same line of mice ( 21 ). However, in contrast, FABP1…”

Section: For Example Fabp1supporting

confidence: 56%

“…FABP1 can, therefore, affect enzyme function by its effect on ligand availability and targeting ( 21 ), modulate enzyme activity by changing membrane structure and fl uidity ( 22 ), and regulate gene expression through activating nuclear receptors ( 23 ). PPARs and FABP1 are both found in the nucleus and likely interact directly as part of a signal transduction pathway ( 23,24 ).…”

Section: Fabp1 As a Cellular Antioxidantmentioning

confidence: 99%

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Recent insights into the biological functions of liver fatty acid binding protein 1

Wang

Bonkovsky

Lemos

et al. 2015

Journal of Lipid Research

194

175

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“…This result was reproducible by others using the same line of mice ( 21 ). However, in contrast, FABP1…”

Section: For Example Fabp1supporting

confidence: 56%

Section: Fabp1 As a Cellular Antioxidantmentioning

confidence: 99%

Recent insights into the biological functions of liver fatty acid binding protein 1

Wang

Bonkovsky

Lemos

et al. 2015

Journal of Lipid Research

194

175

View full text Add to dashboard Cite

“…The level of fat chosen, 45 kcal %, is used to promote obesity in mice [13] using physiologically relevant fat levels and without lowering carbohydrate to an extent that could promote ketogenesis [14]. The body weights were measured weekly, daily food intake and whole-body fat mass determined, and euthanasia performed as described [15]. The studies were approved by and conducted in accordance with Rutgers University Institutional Animal Care and Use Committee policies.…”

Section: Animal and Dietsmentioning

confidence: 99%

Bacterial communities in the small intestine respond differently to those in the caecum and colon in mice fed low- and high-fat diets

Onishi

Campbell

Moreau³

et al. 2017

Microbiology

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Bacterial communities in the mouse caecum and faeces are known to be altered by changes in dietary fat. The microbiota of the mouse small intestine, by contrast, has not been extensively profiled and it is unclear whether small intestinal bacterial communities shift with dietary fat levels. We compared the microbiota in the small intestine, caecum and colon in mice fed a low-fat (LF) or high-fat (HF) diet using 16S rRNA gene sequencing. The relative abundance of major phyla in the small intestine, Bacteriodetes, Firmicutes and Proteobacteria, was similar to that in the caecum and colon; the relative abundance of Verrucomicrobia was significantly reduced in the small intestine compared to the large intestine. Several genera were uniquely detected in the small intestine and included the aerotolerant anaerobe, Lactobacillus spp. The most abundant genera in the small intestine were accounted for by anaerobic bacteria and were identical to those identified in the large intestine. An HF diet was associated with significant weight gain and adiposity and with changes in the bacterial communities throughout the intestine, with changes in the small intestine differing from those in the caecum and colon. Prominent Gram-negative bacteria including genera of the phylum Bacteroidetes and a genus of Proteobacteria significantly changed in the large intestine. The mechanistic links between these changes and the development of obesity, perhaps involving metabolic endotoxemia, remain to be determined.

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“…These mice do not show overt fat malabsorption, indicating that either these FABPs play minor roles in fat absorption or their functions in the process can be compensated for when they are absent. Upon high-fat feeding, mice lacking IFABP showed increased energy expenditure and less weight gain, while mice lacking LFABP showed a propensity to gain weight in a recent study ( 44 ). Other studies, however, found male mice lacking IFABP had greater liver mass and gained more weight under high-fat feeding than wild-type control mice did ( 45,46 ), and mice lacking LFABP were protected against obesity and hepatic steatosis induced by diet without malabsorption of dietary fat ( 47 ).…”

Section: Enzymatic Pathways Mediating Intestinal Tag Synthesismentioning

confidence: 92%

Intestinal triacylglycerol synthesis in fat absorption and systemic energy metabolism

Yen

Nelson

Yen

2015

Journal of Lipid Research

122

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cell membranes. The biosynthesis of TAG thus serves many physiological functions. Because it is highly reduced and anhydrous, TAG is the primary energy substrate stored in adipose tissues to sustain animals during fasting. TAG is also synthesized in the liver for the assembly and secretion of VLDL to transport neutral lipids to other tissues, as well as in the mammary gland for the formation of milk fat globules to deliver fatty acids and other lipid-soluble nutrients to mammalian neonates.In the intestine, TAG synthesis is prominent for its role in the absorption of dietary fat ( 2, 3 ). TAG forms the bulk of animal fats and plant oils. It accounts for approximately 95% of dietary fat, depending on the food sources, with the rest as phospholipids and trace amounts of sterols, lipid-soluble vitamins, and other lipophilic components. Because of its hydrophobicity, TAG does not traverse the cellular membrane. Its absorption involves hydrolysis to fatty acids and monoacylglycerol (MAG) in the intestinal lumen, lipid uptake by the enterocytes, resynthesis of TAG, and assembly and secretion of ApoB-containing chylomicrons for delivery of TAG and other lipid-soluble nutrients. Like with most nutrients, the absorption of TAG occurs mostly in the proximal half of the intestine and less so in the distal intestine, where specifi c compounds, such as bile acids and vitamin B12, are absorbed. The activity of TAG synthesis coincides with the absorption of dietary fat along the length of the intestine, and it is more active in Press, September 17, 2014 DOI 10.1194 Abbreviations: ACSL, acyl-CoA synthetase; AGPAT, 1-acylglycerol-3-phosphate acyltransferase; ATGL, adipose triglyceride lipase; CD36, cluster of differentiation; CGI-58, comparative gene identifi cation-58; Cideb, cell death-inducing DNA fragmentation factor 45 (DFF45) -like effector b; DAG, diacylglycerol; DGAT, acyl-CoA:diacylglycerol acyltransferase; ER, endoplasmic reticulum; FABP, fatty acid binding protein; GLP-1, glucagon-like peptide 1; GPAT, glycerol-3-phosphate acyltransferase; G3P, glycerol-3-phosphate; IFABP, intestine-type fatty acid binding protein; LFABP, liver-type fatty acid binding protein; MAG, monoacylglycerol; MBOAT, membrane-bound O-acyltransferase; MGAT, acylCoA:monoacylglycerol acyltransferase; MTP, microsomal triacylglycerol transfer protein; PA, phosphatidic acid; PAP, phosphatidic acid phosphatase; TAG, triacylglycerol (triglyceride ). Published, JLR Papers in

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Direct Comparison of Mice Null for Liver or Intestinal Fatty Acid-binding Proteins Reveals Highly Divergent Phenotypic Responses to High Fat Feeding

Cited by 48 publications

References 82 publications

Recent insights into the biological functions of liver fatty acid binding protein 1

Recent insights into the biological functions of liver fatty acid binding protein 1

Bacterial communities in the small intestine respond differently to those in the caecum and colon in mice fed low- and high-fat diets

Intestinal triacylglycerol synthesis in fat absorption and systemic energy metabolism

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