Direct Complement Fixation by Turkey and Chicken Serum in Viral Systems.

Brumfield, Helene P.

doi:10.3181/00379727-94-22881

Cited by 22 publications

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“…Nitzschke (1954) noticed that the allantoic fluid harvested from eggs infected with infectious bronchitis virus, when used without concentration, failed to give reliable results. None of the unheated chicken sera in the presence of two complete units of guinea-pig complement showed any anticomplementary activity when tested fresh or after storage for a month at -300 C. Similarly Brumfield & Pomeroy (1957), Tsubahara, Kataoko & Kato (1961), Harada & Matumato (1962) and Kato & Horiuchi (1965) did not find any anticomplementary activity with those unheated sera which were kept frozen at -200 C. before use. Orlans, Rose & Clapp (1962 a), however, found that even fresh fowl serum in a dilution of 1/100 inhibits the lysis of sensitized cells by guinea-pig complement.…”

Section: Discussionmentioning

confidence: 99%

“…was found to be ideal for the test. Brumfield & Pomeroy (1957) employed direct complement fixation with the sera of birds immunized with three viral agents, ornithosis, infectious bronchitis virus and Newcastle disease virus. Immune sera against heterologous antigens within the three systems gave negative results.…”

Section: Discussionmentioning

confidence: 99%

“…(Bushnell & Hudson, 1927;Rice, 1947; Rice, Magwood & Annau, 1960; Nitzschke, 1954Nitzschke, , 1956Brumfield & Pomeroy, 1957). This finding led Rice (1948) to use an indirect complement fixation test for demonstrating antibody in heated avian antisera.…”

Section: Introductionmentioning

confidence: 99%

“…A very few unheated chicken serum samples in the presence of guinea-pig complement (1.8 units) showed very slight anticomplementary activity at low dilutions. Brumfield & Pomeroy (1957) employed sufficient amounts of guinea-pig complement to overcome the anticomplementary effects of unheated turkey or chicken sera. They studied ornithosis, infectious bronchitis and Newcastle disease viruses of birds and showed that the fixation was specific due to antibody rather than to the combined anticomplementary effects.…”

Section: Introductionmentioning

confidence: 99%

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Direct complement fixation test with avian infectious bronchitis virus in chickens

Uppal

1970

J. Hyg.

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SUMMARYThe direct complement fixation test was performed to follow the antibody response in chickens infected with avian infectious bronchitis virus. Concentrated allantoic fluid (4 units) was used as an antigen and allowed to react with serially diluted antiserum in the presence of two complete units of guinea-pig complement for 3 hr. at 40 C. and 2 hr. at 37°C. before the addition of sensitized cells. Serum was unheated and used either fresh or within one month of storage at -300 C. Individual birds showed a rise and fall of complement-fixing antibody both after primary and secondary inoculations. The complement-fixing antibody was detected as early as the seventh day after primary inoculation. The highest complement fixation titre (1/32 to 1/64) was recorded from 14 to 21 days after inoculation with a subsequent gradual decline.The results of the direct complement fixation tests have been correlated with the serum neutralization test. The neutralizing antibodies usually appeared by the 14th day but were not detected at a significant titre until the 21st day after primary inoculation. Serum neutralizing antibodies were still present at high titres even after 7 weeks of infection but the complement-fixing antibodies had disappeared by that time.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Direct complement fixation test with avian infectious bronchitis virus in chickens

Uppal

1970

J. Hyg.

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“…The inability to fix complement may be due to some physical attribute of the Fc portion of the CFI immunoglobulin. An additional point to consider, however, is that failure to demonstrate that CF activity in serum may be due to the heat lability of the immunoglobulin as suggested by Brumfield and Pomeroy(3). The lack of agglutinin activity in CFI antibody suggests that the monkey CFI Ig functions essentially as a univalent antibody.…”

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confidence: 97%

Differences in Clinical and Convalescent-Phase Antibodies of Rhesus Monkeys Infected with Monkey Pox, Tanapox, and Yaba Poxviruses

et al. 1973

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Complement-fixing and complement-fixing inhibiting (CFI) antibodies were demonstrated in the clinical and convalescent stages, respectively, of rhesus monikeys infected with either monkey poxvirus, Tanapoxvirus, or Yaba poxvirus. Specificity of the CFI antibody was confirmed by its failure to cross-react with heterologous poxvirus antigens and by experiments demonstrating the CFI test as being antigen dependent. Serum containing CFI antibody neutralized homologous poxvirus but failed to agglutinate antigen-coated, tanned red blood cells. The application of CFI test as a seroepidemiologic tool for studies of poxvirus infection of man and simian monkeys and the biologic role of CFI antibody in pathogenesis were discussed. sealed in 5-ml ampoules and stored at -70 C.CF and CFI antigens. The preparation of virion and virus-associated antigens from Yaba 539 on August 8, 2020 by guest http://iai.asm.org/ Downloaded from

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Myxoviren

Haagen¹

1964

Viruskrankheiten Des Menschen

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Direct Complement Fixation by Turkey and Chicken Serum in Viral Systems.

Cited by 22 publications

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Direct complement fixation test with avian infectious bronchitis virus in chickens

Direct complement fixation test with avian infectious bronchitis virus in chickens

Differences in Clinical and Convalescent-Phase Antibodies of Rhesus Monkeys Infected with Monkey Pox, Tanapox, and Yaba Poxviruses

Myxoviren

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