Direct cytotoxicity evaluation of 63S bioactive glass and bone-derived hydroxyapatite particles using yeast model and human chondrocyte cells by microcalorimetry

Abstract

In this study, the cytotoxicity evaluation of prepared 63S bioactive glass and bone-derived hydroxyapatite particles with yeast and human chondrocyte cells was carried out using isothermal micro-nano calorimetry (IMNC), which is a new method for studying cell/biomaterial interactions. Bioactive glass particles were made via sol-gel method and hydroxyapatite was obtained from bovine bone. Elemental analysis was carried out by XRF and EDXRF. Amorphous structure of the glass and completely crystalline structure of HA were detected by XRD analysis. Finally, the cytotoxicity of bioactive glass and bone-derived HA particles with yeast and cultured human chondrocyte cells was evaluated using IMNC. The results confirmed the viability, growth and proliferation of human chondrocyte cells in contact with 63S bioactive glass, and bone-derived HA particles. Also the results indicated that yeast model which is much easier to handle, can be considered as a good proxy and can provide a rapid primary estimate of the ranges to be used in assays involving human cells. All of these results confirmed that IMNC is a convenient method which caters to measuring the cell-biomaterial interactions alongside the current methods.

Rice husk derived bioactive glass-ceramic as a functional bioceramic: Synthesis, characterization and biological testing

“…The results from Hafezi et al [38] are in agreement with the bands in the range from 550 to 650 cm −1 , and the band near 1040 cm − 1 corresponds to a P-O group described in a related study. The bands at 1487 and 1418 cm −1 formed after the bioactivity test and are related to the C-O stretching of the carbonate group [39][40][41]. Additionally, the band at 873 cm −1 is related to C-O stretching, which formed for a similar sample after immersion in the SBF [42].…”

Characterization and Stability of Tanshinone IIA Solid Dispersions with Hydroxyapatite

“…Furthermore HAp has been applied widely in various biomedical applications, owning to its unique functional properties of high surface-area-to-volume ratio and porosity. Its ultrafine structure, which is similar to biological apatite’s, had a great impact on cell-biomaterial interaction [32,33,34]. However, HAp has not yet been reported as a carrier of solid dispersions.…”

Synthesis of ternary bioactive glass derived aerogel and xerogel: study of their structure and bioactivity

“…Glass powders were synthesized through a sol-gel process. Xerogel bioactive glasses (labelled as XG) were prepared in a similar way as previously described by other authors (Doostmohamadi et al 2011). Briefly, tetraethylorthosilicate (TEOS), which was selected as a silica precursor for the sol, was added to ethanol as an alcoholic media and the mixture was stirred for 30 min.…”