Direct Cytotoxicity of &lt;i&gt;Lentinula edodes&lt;/i&gt; Mycelia Extract on Human Hepatocellular Carcinoma Cell Line

Yukawa, Hiroshi; Ishikawa, Satoshi; Kawanishi, Takashi; Tamesada, Makoto; Tomi, Hironori

doi:10.1248/bpb.b110657

Cited by 18 publications

(16 citation statements)

References 37 publications

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“…Present studies revealed that BE3 consists mainly of ribonucleic acid (59.1%). 59,60 Sine the anticancer action of FA-2-b-β and LEM are in the same vein as changes caused by the BE3 fraction, it may also confirm the importance of ribonucleic acids presented in BE3 for its chemoprevention abilities. 54,55 Among the mentioned studies, the detailed molecular mechanism of the anticancer action was described only by Sun et al 54 They discovered that FA-2-b-β (RNAprotein complex isolated from A. blazei) in combination with AZT (3′-azido-3′-deoxythymidine) induced apoptosis in the human gastric cancer cell line MKN45 which was associated with inhibition of tumor cell telomerase as well as BCL2 down-regulation and up-regulation of caspase 3 mRNA.…”

Section: Discussionmentioning

confidence: 76%

Boletus edulis ribonucleic acid – a potent apoptosis inducer in human colon adenocarcinoma cells

Lemieszek¹,

Ribeiro

Alves

et al. 2016

Food Funct.

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Despite the large popularity of the Boletus edulis mushroom, little is known about its influence on human health and the possibilities of its therapeutic use. Nevertheless, several reports revealed the usefulness of biopolymers isolated from it in cancer treatment. Our previous studies have shown that B. edulis water soluble biopolymers are not toxic against normal colon epithelial cells (CCD841 CoTr) and at the same concentration range elicited a very prominent antiproliferative effect in colon cancer cells (LS180) which was accompanied with cell cycle arrest in the G0/G1 phase. The purpose of the present study was to verify the proapoptotic properties of a selected fraction from B. edulis -BE3, as well as determine its chemical nature. The BE3 fraction was extracted with hot water and purified by anion-exchange chromatography. Further chemical examinations revealed that BE3 consists mainly of ribonucleic acid (59.1%).The ability of BE3 to induce programmed cell death was examined in human colon cancer cell lines LS180 and HT-29 by measuring caspase activation, DNA fragmentation and expression of BAX, BCL2, TP53 and CDKN1A genes. The sensitivity of colon cancer cells with silenced BAX, TP53 and CDKN1A expression to BE3 treatment was also evaluated. We have demonstrated for the first time that the BE3 fraction is a potent apoptosis inducer in human colon cancer cells. The revealed mechanism of apoptosis triggering was dependent on the presence of functional p53 and consequently was a little different in investigated cell lines. Our results indicated that BE3 stimulated proapoptotic genes BAX (LS180, HT-29), TP53 (LS180) and CDKN1A (HT-29) while at the same time silenced the expression of the key prosurvival gene BCL2 (LS180, HT-29). The obtained results indicate the high therapeutic potential of the BE3 fraction against colon cancer, yet it is necessary to further confirm fraction efficacy and safety in animal and clinical studies.

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Section: Discussionmentioning

confidence: 76%

Boletus edulis ribonucleic acid – a potent apoptosis inducer in human colon adenocarcinoma cells

Lemieszek¹,

Ribeiro

Alves

et al. 2016

Food Funct.

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“…The low temperature (<50°C) aqueous extract of this mushroom significantly decreased proliferation of the human tumor cell lines laryngeal carcinoma (HEp-2) and cervical adenocarcinoma (HeLA), with IC 50% values ranging from 0.46% to 1.03% [13]. Yukawa et al, [22] examined the antiproliferative effect of L. edodes mycelia extract on HepG2 human hepatocellular carcinoma cells. The extract efficaciously induced apoptosis of tested type of cells through the caspase -3 and -8 death receptor pathways [22].…”

Section: Ganoderma Lucidum Commonly Known As Lingzhi Ormentioning

confidence: 99%

“…Yukawa et al, [22] examined the antiproliferative effect of L. edodes mycelia extract on HepG2 human hepatocellular carcinoma cells. The extract efficaciously induced apoptosis of tested type of cells through the caspase -3 and -8 death receptor pathways [22].…”

Section: Ganoderma Lucidum Commonly Known As Lingzhi Ormentioning

confidence: 99%

Mycotherapy of Cancer: An Update on Cytotoxic and Antitumor Activities of Mushrooms, Bioactive Principles and Molecular Mechanisms of their Action

Popović¹,

Živković²,

Davidović³

et al. 2013

CTMC

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Mycotherapy is defined as the study of the use of extracts and compounds obtained from mushrooms as medicines or health-promoting agents. The present review updates the recent findings on anticancer/antitumor agents derived from mushroom extracts and their metabolites. The increasing number of studies in the past few years revealed mushroom extracts as potent antitumor agents. Also, numerous studies were conducted on bioactive compounds isolated from mushrooms reporting the heteropolysaccharides, β-glucans, α-glucans, proteins, complexes of polysaccharides with proteins, fatty acids, nucleoside antagonists, terpenoids, sesquiterpenes, lanostanoids, sterols and phenolic acids as promising antitumor agents. Also, molecular mechanisms of cytotoxicity against different cancer cell lines are discussed in this review. Findings with Antrodia camphorata and Ganoderma lucidium extracts and isolated compounds are presented, as being the most deeply studied previously.

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“…Mushrooms are a good source of natural compounds for cancer treatments. Many edible mushrooms and their products have been noted as dietary supplements and sources of medicinal compounds with potential anticancer properties against several types of cancer in human (Ferrari et al, 2012;Yukawa et al, 2012. ) L. edodes is one of the medicinal mushrooms which was studied and showed numerous pharmacological properties, such as antimicrobial, antiviral, antioxidant, anti-inflammatory, antiatherogenic, hypoglycemic, hepatoprotective, anticancer, and immunomodulating properties (Ferreira et al, 2012;Rai et al, 2005;Zaidman et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…) L. edodes is one of the medicinal mushrooms which was studied and showed numerous pharmacological properties, such as antimicrobial, antiviral, antioxidant, anti-inflammatory, antiatherogenic, hypoglycemic, hepatoprotective, anticancer, and immunomodulating properties (Ferreira et al, 2012;Rai et al, 2005;Zaidman et al, 2005). Several compounds extracted from L. edodes such as Lentinan (Chihara et al, 1970;Zhang et al, 2007), the polysaccharide L-II (Zheng et al, 2005), water extracts of fruiting bodies (Israilides et al, 2008), L•E•M (Shen et al, 2009), and LEP (Yukawa et al, 2012), show immunomodulatory and anticancer activity.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the cytotoxic effect of crude aqueous and ethanolic extracts isolated from Lentinus spp. on human cancer cell lines

Udchumpisa¹,

Bangyeekhun.²

2019

MJM

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The aim of this study was to evaluate some chemical properties and the cytotoxic effect of aqueous and ethanolic crude polysaccharides extracted from five Lentinus sp. on human cancer cell lines. It was hypothesized that other species of the genus Lentinus could show the pharmacological actions as presence in Lentinus edodes, especially anticancer properties. Methodology and results: Crude extracts of dried fruit bodies and mycelia from L. edodes, Lentinus sajor-caju, Lentinus swartzii, Lentinus squarrosulus and Lentinus velutinus were extracted using two solvents, hot water and 95% ethanol, and evaluated for their total carbohydrates, proteins, reducing sugar, phenol contents, and cytotoxicity. The yield of crude extracts was 33.6-205.3 mg/g dry weight of a sample. Cytotoxicity was determined with 10 mg/mL of crude aqueous and 1 mg/mL of crude ethanolic extracts by using the [3-(4,5-dimethylthiazol-2-yl)-2, 5diphenyltetrazolium bromide] (MTT) method. All extracts showed non-cytotoxicity against the normal cell lines, LLC-MK2 and L929 cells. While, the extracts of L. edodes, L. sajor-caju, L. squarrosulus and L. velutinus displayed the cytotoxicity against the human cancer cell lines. Conclusion, significance and impact study: The crude aqueous and ethanolic extract from fruit bodies of L. velutinus and the ethanolic extract from fruit bodies of L. sajor-caju and L. squarrosulus displayed the adverse effect against the human cancer cell lines. Hence, these extracts are a potential source of bioactive compounds for cancer treatment.

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Direct Cytotoxicity of <i>Lentinula edodes</i> Mycelia Extract on Human Hepatocellular Carcinoma Cell Line

Cited by 18 publications

References 37 publications

Boletus edulis ribonucleic acid – a potent apoptosis inducer in human colon adenocarcinoma cells

Boletus edulis ribonucleic acid – a potent apoptosis inducer in human colon adenocarcinoma cells

Mycotherapy of Cancer: An Update on Cytotoxic and Antitumor Activities of Mushrooms, Bioactive Principles and Molecular Mechanisms of their Action

Evaluation of the cytotoxic effect of crude aqueous and ethanolic extracts isolated from Lentinus spp. on human cancer cell lines

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