Direct demonstration of termination signals for RNA polymerase II from the sea urchin H2A histone gene

Briggs, D.; Jackson, Dean A.; Whitelaw, Emma; Proudfoot, Nicholas J.

doi:10.1093/nar/17.20.8061

Cited by 16 publications

(10 citation statements)

References 23 publications

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“…In both systems the termination site does not require a histone 3'-processing signal. The termination site which they defined is capable of blocking expression of a human gene in the absence of the histone 3'-processing signal, suggesting that this is an intrinsic termination signal (8). The physiological significance of this sequence is not clear, given the heterologous nature of these experiments.…”

Section: Discussionmentioning

confidence: 96%

“…They suggested that termination of transcription in the sea urchin H2a gene injected into Xenopus oocytes occurred about 200 nt downstream of the gene. This same sequence has been shown to terminate transcription of heterologous genes in mammalian cells in the absence of a 3'-processing signal (8,25), suggesting that the mechanism of transcription termination may differ between histone genes and genes encoding polyadenylated mRNAs.…”

mentioning

confidence: 89%

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An intact histone 3'-processing site is required for transcription termination in a mouse histone H2a gene.

Chodchoy¹,

Pandey²,

Marzluff³

1991

Mol. Cell. Biol.

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A transcription termination site has been characterized between the mouse histone H2a-614 and H3-614 genes. There is a poly(A)- RNA present in small amounts in the nucleus which ends 600 nucleotides 3' to the H2a-614 gene. Nuclear transcription studies demonstrate that transcription extends at least 600 nucleotides 3' to the gene but is greatly reduced 700 nucleotides 3' to the gene. If all or part of the normal 3'-processing signal, consisting of the stem-loop and the U7 small nuclear ribonucleoprotein binding site, is deleted, transcription then continues past the putative termination site and RNAs which end at the 3' end of the downstream H3-614 gene accumulate. Insertion of a 150-nucleotide fragment containing the termination site between the histone 3' end and downstream polyadenylation sites reduces usage of polyadenylation sites 85 to 90%. Taken together these results suggest there is a transcription termination site which requires an intact histone 3'-processing signal to function.

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Section: Discussionmentioning

confidence: 96%

mentioning

confidence: 89%

An intact histone 3'-processing site is required for transcription termination in a mouse histone H2a gene.

Chodchoy¹,

Pandey²,

Marzluff³

1991

Mol. Cell. Biol.

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“…Cytoplasmic RNA was purified by centrifugation through a sucrose cushion, phenol-chloroform extraction and ethanol precipitation. S1 analysis was carried out as described [28], using a 3'end-labeled double-strand DNA probes as described in Fig. 2.…”

Section: Transfection and S1-nuclease Analysismentioning

confidence: 99%

Expression of the early lymphocyte activation antigen CD69, a C‐type lectin, is regulated by mRNA degradation associated with AU‐rich sequence motifs

et al. 1995

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CD69 is the earliest inducible cell surface glycoprotein acquired during lymphoid activation both in vitro and in vivo under physiological conditions and inflammation. This molecule is involved in lymphocyte proliferation, and functions as a signal-transmitting receptor in T and B lymphocytes, NK cells and platelets. Molecular cloning of CD69 cDNA revealed that this antigen is a type-II integral membrane protein with a C-type lectin domain in the extracellular region. The expression time course of CD69 mRNA has previously been reported to be transient, peaking around 3 h after induction in T lymphocytes, and declining to nearly resting levels by 8 h. We describe herein studies on the stability of the CD69 mRNA in phorbol ester-activated T lymphocytes. The level of CD69 mRNA in these cells declined rapidly with a half-life of less than 60 min. This finding is consistent with the presence of several AU-rich sequence motifs in the 3' untranslated region (3'UTR), which have been implicated in the selective destabilization of short-lived mRNA of mammalian cytokines, and proto-oncogenes. We have therefore introduced a fragment of the 3'UTR of the human CD69 cDNA, which contains the AU-rich sequence motifs, into the 3'UTR of the rabbit beta-globin gene. This inserted sequence causes the otherwise stable beta-globin mRNA to become unstable in vivo. A similar destabilizing effect is observed when the 51-nucleotide AU sequence from the mRNA of the human cytokine granulocyte/macrophage colony-stimulating factor is used as a positive control. Furthermore, the introduction of 194-bp fragment from the CD69 3'UTR containing most of the AU-rich motifs was sufficient to induce the destabilizing effect. We propose that the selective degradation pathway involved in the regulation of the expression of cytokines and proto-oncogenes is implicated in the rapid degradation of CD69 mRNA in activated T lymphocytes. This pathway may constitute a general mechanism to regulate the expression of inducible molecules involved in inflammatory processes.

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“…In addition to the wellstudied effects in c-myc, which formed the starting point for our work, similar mechanisms appear to control transcription of c-myb (3), c-mos (48), c-fos (18,50), L-myc (36), and the adenosine deaminase gene (14). Premature (12,15), and in small nuclear RNA (snRNA) genes (28,54). However, no consensus sequence for a pol II termination site has emerged from these studies.…”

mentioning

confidence: 92%

Analysis of premature termination in c-myc during transcription by RNA polymerase II in a HeLa nuclear extract.

London¹,

Keene²,

Landick³

1991

Mol. Cell. Biol.

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Transcriptional regulation of the human c-myc gene, an important aspect of cellular differentiation, occurs in part at the level of transcript elongation. In vivo, transcriptional arrest, due to either pausing or termination, occurs near the junction between the first exon and first intron and varies with the growth state of the cell. We have tested the transcription of c-myc templates in HeLa nuclear extracts. We did not observe significant arrest under standard conditions, but we found that a considerable fraction of transcription complexes stopped at the c-myc TII site (just past the first exon-intron junction) when the KCI concentration was raised to 400 mM during elongation. Transcriptional arrest at TII also was observed at KCI concentrations as low as 130 mM and when potassium acetate or potassium glutamate was substituted for KCI. Under these conditions, arrest occurred at the T1I site when transcription was initiated at either the c-myc P2 promoter or the adenovirus 2 major late promoter. Further, the TII sequence itself, in forward but not reverse orientation, was sufficient to stop transcription in a HeLa nuclear extract. By separating the TII RNA from active transcription complexes by using gel filtration, we found that arrest at TII at 400 mM KCI resulted in transcript release and thus true transcriptional termination. The efficiency of termination at TII depended on the growth state of the celHs from which the extracts were made, suggesting that some factor or factors control premature termination in c-myc.

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Direct demonstration of termination signals for RNA polymerase II from the sea urchin H2A histone gene

Cited by 16 publications

References 23 publications

An intact histone 3'-processing site is required for transcription termination in a mouse histone H2a gene.

An intact histone 3'-processing site is required for transcription termination in a mouse histone H2a gene.

Expression of the early lymphocyte activation antigen CD69, a C‐type lectin, is regulated by mRNA degradation associated with AU‐rich sequence motifs

Analysis of premature termination in c-myc during transcription by RNA polymerase II in a HeLa nuclear extract.

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