Direct detection of carbapenemase-associated proteins of Acinetobacter baumannii using nanodiamonds coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Chang, Kai‐Chih; Chung, Chin-Yi; Yeh, Chen-Hsing; Hsu, Kuo-Hsiu; Chin, Ya-Ching; Huang, Sin-Siang; Liu, Bo-Rong; Chen, Hsi-An; Hu, Anren; Soo, Po-Chi; Peng, Wen-Ping

doi:10.1016/j.mimet.2018.02.014

Cited by 17 publications

(8 citation statements)

References 49 publications

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“…Among 51 carbapenem-resistant A. baumannii strains, 49 showed a signal at 40,279 ± 87 m/z, whereas four out of 15 carbapenem-susceptible strains showed a signal at the same m/z. The sensitivity and specificity were 96 and 73% in comparison to the microdilution imipenem susceptibility testing, which provides MIC determination (Chang et al, 2018).…”

Section: Identification Of Biomarkers Associated With Drug-resistancementioning

confidence: 96%

Detection of Antibiotic-Resistance by MALDI-TOF Mass Spectrometry: An Expanding Area

Florio

Baldeschi

Rizzato

et al. 2020

Front. Cell. Infect. Microbiol.

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Several MALDI-TOF MS-based methods have been proposed for rapid detection of antimicrobial resistance. The most widely studied methods include assessment of β-lactamase activity by visualizing the hydrolysis of the β-lactam ring, detection of biomarkers responsible for or correlated with drug-resistance/non-susceptibility, and the comparison of proteomic profiles of bacteria incubated with or without antimicrobial drugs. Antimicrobial-resistance to a number of antibiotics belonging to different classes has been successfully tested by MALDI-TOF MS in a variety of clinically relevant bacterial species including members of Enterobacteriaceae family, non-fermenting Gram-negative bacteria, Gram-positive cocci, anaerobic bacteria and mycobacteria, opening this field to further clinically important developments. Early detection of drug-resistance by MALDI-TOF MS can be particularly helpful for clinicians to streamline the antibiotic therapy for a better outcome of patients with systemic infection, in all cases where a prompt and effective antibiotic treatment is essential to preserve organ function and/or patient survival.

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Section: Identification Of Biomarkers Associated With Drug-resistancementioning

confidence: 96%

Detection of Antibiotic-Resistance by MALDI-TOF Mass Spectrometry: An Expanding Area

Florio

Baldeschi

Rizzato

et al. 2020

Front. Cell. Infect. Microbiol.

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“…An extended-spectrum class C beta-lactamase from A. baumannii was recently identified ( m/z ~40,279) as a biomarker for carbapenem resistance. The sensitivity and specificity of the method were 96% and 73%, respectively, compared with the microdilution imipenem susceptibility testing method [ 103 ]. An 11,109-Da peak was identified by MALDI-TOF MS to distinguish the protein patterns of Klebsiella pneumoniae carbapenemase (KPC)-producing and non-KPC-producing K. pneumoniae strains [ 104 ].…”

Section: Accuracy For Microbial Identification and Other Applicationmentioning

confidence: 99%

Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) Analysis for the Identification of Pathogenic Microorganisms: A Review

et al. 2021

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Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used in the field of clinical microbiology since 2010. Compared with the traditional technique of biochemical identification, MALDI-TOF MS has many advantages, including convenience, speed, accuracy, and low cost. The accuracy and speed of identification using MALDI-TOF MS have been increasing with the development of sample preparation, database enrichment, and algorithm optimization. MALDI-TOF MS has shown promising results in identifying cultured colonies and rapidly detecting samples. MALDI-TOF MS has critical research applications for the rapid detection of highly virulent and drug-resistant pathogens. Here we present a scientific review that evaluates the performance of MALDI-TOF MS in identifying clinical pathogenic microorganisms. MALDI-TOF MS is a promising tool in identifying clinical microorganisms, although some aspects still require improvement.

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“…A more elaborate sample preparation of periplasmic proteins allowed the detection of CMY-2 cephalosporinases ( m/z 39,850) in various Enterobacteriaceae, however, not in all strains positive for CMY-2 by reference methods (Papagiannitsis et al, 2014). With a sample preparation using detonation nanodiamonds, Chang et al (2018) were able to detect carbapenemases in Acinetobacter baumannii isolates ( m/z ∼40,279 ± 87), although 4 out of 15 susceptible strains showed also a signal in this mass range. With a moderately complex sample preparation procedure, Espinosa et al (2018) achieved the detection of CMY-2 carbapenemases ( m/z 39,800) in E. coli by MALDI-TOF MS.…”

Section: Detection Of Proteins Conferring Resistancementioning

confidence: 99%

One System for All: Is Mass Spectrometry a Future Alternative for Conventional Antibiotic Susceptibility Testing?

Welker

Belkum

2019

Front. Microbiol.

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The two main pillars of clinical microbiological diagnostics are the identification of potentially pathogenic microorganisms from patient samples and the testing for antibiotic susceptibility (AST) to allow efficient treatment with active antimicrobial agents. While routine microbial species identification is increasingly performed with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), routine AST still largely relies on conventional and molecular techniques such as broth microdilution or disk and gradient diffusion tests, PCR and automated variants thereof. However, shortly after the introduction of MALDI-TOF MS based routine identification, first attempts to perform AST on the same instruments were reported. Today, a number of different approaches to perform AST with MALDI-TOF MS and other MS techniques have been proposed, some restricted to particular microbial taxa and resistance mechanisms while others being more generic. Further, while some of the methods are in a stage of proof of principles, others are already commercialized. In this review we discuss the different principal approaches of mass spectrometry based AST and evaluate the advantages and disadvantages compared to conventional and molecular techniques. At present, the possibility that MS will soon become a routine tool for AST seems unlikely – still, the same was true for routine microbial identification a mere 15 years ago.

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Direct detection of carbapenemase-associated proteins of Acinetobacter baumannii using nanodiamonds coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Cited by 17 publications

References 49 publications

Detection of Antibiotic-Resistance by MALDI-TOF Mass Spectrometry: An Expanding Area

Detection of Antibiotic-Resistance by MALDI-TOF Mass Spectrometry: An Expanding Area

Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) Analysis for the Identification of Pathogenic Microorganisms: A Review

One System for All: Is Mass Spectrometry a Future Alternative for Conventional Antibiotic Susceptibility Testing?

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