Direct detection of morphine in human urine by surface-ionization mass spectrometry

Abstract

Detection and analysis of drugs-of-abuse in biological fluids are the essential tasks for the law enforcement, forensic toxicology, doping research and social health. In this work, we developed a surface-ionization mass spectrometry method for direct detection of trace amount of morphine spiked in blank urine. The mass-spectrometric analysis of spiked samples was carried out without preliminary extraction and chromatographic separation. It was found out that the major fragment ions at m/z 144, 146 could be served as indicator lines of morphine spiked in blank urine. Limit of detection of morphine spiked in blank urine was 100 pg (50 ng/ml), and a linear range of calibration curve was more than two orders of magnitude. The spiked samples were also analyzed by gas chromatography–mass spectrometry without preliminary extraction and derivatization procedures. No morphine was found in the spiked samples. The experimental data show that the high selectivity of the surface ionization can be allowed to direct analysis of morphine spiked in blank urine without its preliminary extraction and chromatographic separation.

Development of surface ionization mass spectrometry for detection of stimulants in human urine

“…In our previous work, urine spiked with morphine spiked was analyzed by SI/MS. 42 It was found that a major peak of the analyte in the mass spectrum could be served as an indicator line in biofluids. Other detected peaks were products of deep fragmentation of cocaine on the heated surface as well as some peaks such as with m/z 86, 112, and 126 were related to urine ingredients.…”

Examination of the effect of air atmosphere on heterogeneous reactions under surface ionization of psychotropic drug molecules

“…Along with these requirements, the emitters with the high work function for their stable operation in time and with high fluxes of organic compounds should be used as an ion source. 16 For the SI studies of organic compounds, the static magnetic, 25 time-of-flight (TOF) 26 and quadrupole 21,27 mass spectrometers are also used. The static magnetic mass spectrometers allow analysis of monomolecular decays of vibrationally excited maternal ions.…”

“…14,17 By now, a large number of organic and bioorganic compounds on emitters from various materials have been mass-spectrometrically investigated under vacuum conditions. 18–21 It was found that the molecules of organic solvents (ketones, aldehydes, alcohols, simple hydrocarbons) and simple gases (H 2 , N 2 , CO 2 , CO, H 2 O) are almost not ionized by SI. The method has a high selectivity to the ionization energy of the compounds.…”

Исследование Быстродействия Поверхностно-Ионизационных Детекторов

“…Как известно, важнейшими параметрами детекторов являются их чувствительность, селективность, линейно динамический диапазон (ЛДД), стабильность и быстродействие [17,[29][30][31][32][33][34]. На данный момент существует большое количество научно-практических работ, посвященных выявлению закономерностей ПИ и исследованию физико-химических свойств органических и биоорганических соединений [22][23][24][25][26][27][28], поиску эффективных и эмиссионно-стабильных ПИ эмиттеров для детекторов [35][36][37][38][39], определению чувствительности и ЛДД ПИ детекторов к веществам [17][18][19][20][21][25][26][27][28], разработке методов обнаружения и анализа веществ в различных биологических образцах на основе ПИ детекторов [40][41][42][43][44][45][46][47]. Несмотря на это, отсутствуют сведения о закономерностях быстродействия ПИ детекторов.…”