Direct detection of rhinoviruses by an enzyme‐linked immunosorbent assay

Dearden, C J; Al‐Nakib, W.

doi:10.1002/jmv.1890230211

Cited by 14 publications

(3 citation statements)

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“…Such procedures are time consuming, labour intensive and require considerable expertise. Although, it is now possible to detect rhinovirus antigens directly in nasal washings using immunologically based methods such as ELISA [10], the diversity of serotypes, recently estimated to be around 100 [12] makes efficient detection of all serotypes difficult. We have therefore, attempted to overcome this problem by developing procedures based on R N A detection.…”

Section: Introductionmentioning

confidence: 99%

Rhinovirus detection using probes from the 5′ and 3′ end of the genome

Forsyth

Al‐Nakib

Chadwick

et al. 1989

Archives of Virology

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This study investigated the abilities of cDNA probes from the 5' and 3' ends of the genome of human rhinoviruses (HRV-) 14, 9, and 1B to detect RNA from 59 rhinovirus serotypes. The results show that probes from the 5' end of the genomes of HRV-14, 9, and 1 B detected a large number of serotypes but the detection rate was variable and depended on the degree of homology with the particular probe. In contrast, all the 3' end probes were specific for the homologous virus. However, a long HRV-9 probe detected a large number of serotypes. It was concluded that such cDNA probes would not detect all serotypes with equal efficiency. Synthetic oligonucleotides corresponding to short but highly conserved regions in the 5' non coding region may overcome this problem.

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Section: Introductionmentioning

confidence: 99%

Rhinovirus detection using probes from the 5′ and 3′ end of the genome

Forsyth

Al‐Nakib

Chadwick

et al. 1989

Archives of Virology

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“…A number of respiratory viruses, such as respiratory syncytial virus (RSV), influenza, parainfluenza, and adenoviruses, can now be detected rapidly by either immunofluorescence or ELISA. An ELISA has recently been developed to detect HRV directly in nasal washings but the test was found to be generally serotype specific [3]. Because of the large number of H R V serotypes, such assays are not useful in the general diagnosis of H R V infection.…”

Section: Introductionmentioning

confidence: 99%

Use of synthetic oligonucleotide probes to detect rhinovirus RNA

Bruce

Al‐Nakib

Almond

et al. 1989

Archives of Virology

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Current methods of detecting a human rhinovirus (HRV) infection are either based on isolation of virus in appropriate susceptible cell lines, which is time-consuming and requires considerable expertise, or are dependent on knowing the serotype. The existence of over 100 immunologically distinct serotypes makes serotype specific assays, such as ELISA, unsuitable for general diagnostic assays. In this study a general rhinovirus assay is described which utilises synthetic oligonucleotides as probes in a filter hybridization assay. The probes are designed to bind to short but highly conserved regions of the rhinovirus genome. Indeed, the probes successfully detected all 57 rhinovirus serotypes tested. Furthermore, the test was used to demonstrate rhinovirus infection in clinical samples from 57 volunteers, inoculated with HRV, collected on six consecutive days. Clinical samples were taken prior to inoculation and on days 2-7 after inoculation. The filter hybridization assay gave results comparable to virus culture on days 2 and 3 post-inoculation, but was more sensitive on subsequent days.

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“…Efforts to develop antibodies with multiserotype recognition have not been successful because the epitopes targeted were exterior regions of the viral capsid (Al-Nakib et al, 1986;Dearden and Al-Nakib, 1987;McCray and Werner, 1987). These regions are highly variable and antibodies to the most immunodominant epitopes are often serotype specific (Sherry and Rueckert, 1985;Sherry et al, 1986).…”

Section: Introductionmentioning

confidence: 99%