Direct Determination of Bacterial Cell Viability Using Carbon Nanotubes Modified Screen‐printed Electrodes

Hassan, Rabeay Y. A.; Wollenberger, Ulla

doi:10.1002/elan.201900047

Cited by 21 publications

(11 citation statements)

References 26 publications

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“…Cell wall components of microorganisms and pigments could have active metal sorption sites that are able to accumulate cadmium and lead simultaneously [ 26 , 27 , 28 , 29 ]. Amongst biosensors used techniques for online evaluation of microbial activity and intra/extracellular functions, microbial electrochemical systems (MESs) were developed and applied in many applications [ 30 , 31 , 32 , 33 , 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

Microbial Sensing and Removal of Heavy Metals: Bioelectrochemical Detection and Removal of Chromium(VI) and Cadmium(II)

et al. 2021

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The presence of inorganic pollutants such as Cadmium(II) and Chromium(VI) could destroy our environment and ecosystem. To overcome this problem, much attention was directed to microbial technology, whereas some microorganisms could resist the toxic effects and decrease pollutants concentration while the microbial viability is sustained. Therefore, we built up a complementary strategy to study the biofilm formation of isolated strains under the stress of heavy metals. As target resistive organisms, Rhizobium-MAP7 and Rhodotorula ALT72 were identified. However, Pontoea agglumerans strains were exploited as the susceptible organism to the heavy metal exposure. Among the methods of sensing and analysis, bioelectrochemical measurements showed the most effective tools to study the susceptibility and resistivity to the heavy metals. The tested Rhizobium strain showed higher ability of removal of heavy metals and more resistive to metals ions since its cell viability was not strongly inhibited by the toxic metal ions over various concentrations. On the other hand, electrochemically active biofilm exhibited higher bioelectrochemical signals in presence of heavy metals ions. So by using the two strains, especially Rhizobium-MAP7, the detection and removal of heavy metals Cr(VI) and Cd(II) is highly supported and recommended.

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Section: Introductionmentioning

confidence: 99%

Microbial Sensing and Removal of Heavy Metals: Bioelectrochemical Detection and Removal of Chromium(VI) and Cadmium(II)

et al. 2021

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“…Hence, earlier efforts were made to employ the measurement of dissolved oxygen consumption by living cells as a direct measure of the microbial survival [ 7 ]. Consequently, the electron transfer process from living-microorganisms towards electrodes in MES is exploited in microbial fuel cells [ 8 , 9 ] or diagnostic tools for rapid assessment of microbial activity [ 10 , 11 , 12 ]. In these regards, many MES approaches were designed and tested for biological purposes [ 13 , 14 , 15 , 16 ].…”

Section: Principles Of Microbial Electrochemical Systemsmentioning

confidence: 99%

“…Instead of applying biological recognition elements, such as antibodies, the online-measuring of cell viability with the ability to distinguish living from the dead cells can be achieved through the use of MESs [ 1 , 12 ]. MESs could be used effectively to distinguish between live and dead microbial cells, monitor the intracellular redox functions of living cells, and characterize electrochemically active biofilms.…”

Section: Biosensing Applications Of Mess For Microbial Detectionmentioning

confidence: 99%

Microbial Electrochemical Systems: Principles, Construction and Biosensing Applications

Hassan

Febbraio

Andreescu

2021

Sensors

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Microbial electrochemical systems are a fast emerging technology that use microorganisms to harvest the chemical energy from bioorganic materials to produce electrical power. Due to their flexibility and the wide variety of materials that can be used as a source, these devices show promise for applications in many fields including energy, environment and sensing. Microbial electrochemical systems rely on the integration of microbial cells, bioelectrochemistry, material science and electrochemical technologies to achieve effective conversion of the chemical energy stored in organic materials into electrical power. Therefore, the interaction between microorganisms and electrodes and their operation at physiological important potentials are critical for their development. This article provides an overview of the principles and applications of microbial electrochemical systems, their development status and potential for implementation in the biosensing field. It also provides a discussion of the recent developments in the selection of electrode materials to improve electron transfer using nanomaterials along with challenges for achieving practical implementation, and examples of applications in the biosensing field.

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“…In this respect, biosensors provided a diagnostic tool either for viruses or for neutralizing antibodies in many trials by obtaining signals, which, in the best scenario, were directly proportional to the viral load or concentration of the analytes [85][86][87]. To introduce the reader in biosensor-based diagnosis and monitoring of viral diseases, in this section we briefly review a few biosensing devices, which have been developed for previous viral outbreaks.…”

Section: Point-of-care and Standalone Biosensing Devicesmentioning

confidence: 99%

Point-of-Care Diagnostics of COVID-19: From Current Work to Future Perspectives

Hussein

Hassan

Chino

et al. 2020

Sensors

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Coronaviruses have received global concern since 2003, when an outbreak caused by SARS-CoV emerged in China. Later on, in 2012, the Middle-East respiratory syndrome spread in Saudi Arabia, caused by MERS-CoV. Currently, the global crisis is caused by the pandemic SARS-CoV-2, which belongs to the same lineage of SARS-CoV. In response to the urgent need of diagnostic tools, several lab-based and biosensing techniques have been proposed so far. Five main areas have been individuated and discussed in terms of their strengths and weaknesses. The cell-culture detection and the microneutralization tests are still considered highly reliable methods. The genetic screening, featuring the well-established Real-time polymerase chain reaction (RT-PCR), represents the gold standard for virus detection in nasopharyngeal swabs. On the other side, immunoassays were developed, either by screening/antigen recognition of IgM/IgG or by detecting the whole virus, in blood and sera. Next, proteomic mass-spectrometry (MS)-based methodologies have also been proposed for the analysis of swab samples. Finally, virus-biosensing devices were efficiently designed. Both electrochemical immunosensors and eye-based technologies have been described, showing detection times lower than 10 min after swab introduction. Alternative to swab-based techniques, lateral flow point-of-care immunoassays are already commercially available for the analysis of blood samples. Such biosensing devices hold the advantage of being portable for on-site testing in hospitals, airports, and hotspots, virtually without any sample treatment or complicated lab precautions.

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Direct Determination of Bacterial Cell Viability Using Carbon Nanotubes Modified Screen‐printed Electrodes

Cited by 21 publications

References 26 publications

Microbial Sensing and Removal of Heavy Metals: Bioelectrochemical Detection and Removal of Chromium(VI) and Cadmium(II)

Microbial Sensing and Removal of Heavy Metals: Bioelectrochemical Detection and Removal of Chromium(VI) and Cadmium(II)

Microbial Electrochemical Systems: Principles, Construction and Biosensing Applications

Point-of-Care Diagnostics of COVID-19: From Current Work to Future Perspectives

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