Direct Determination of NO2-Substituted Pyrenes by Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Mass Spectrometry

Moriwaki, Hiroshi; Funasaka, Kunihiro; Uebori, Michiko

doi:10.2116/analsci.16.1247

Cited by 15 publications

(5 citation statements)

References 8 publications

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“…Table 4 lists the nitro-PAH recovery rates of the overall analytical procedure (extraction, clean-up, and HPLC analysis) applied to spiked spark discharge soot samples (70-90% for the 12 measured compounds) and from standard diesel soot samples (80-90% for the four compounds listed in the original NIST Certificate of Analysis). The values are similar to those of the corresponding parent PAH investigated in this study (80-95%, [36]) and of nitro-PAH investigated in earlier studies (80-100%) using ultrasonic extraction [21] or different techniques like pressurized fluid extraction (PFE) and soxhlet extraction [29,47,48]. Due to the relatively high recovery rates and the lack of a suitable internal standard, no recovery correction was applied to the nitro-PAH measurement values reported below.…”

Section: Resultssupporting

confidence: 85%

“…Analysis of nitrated polycyclic aromatic hydrocarbons by liquid chromatography with fluorescence and mass spectrometry detection: air particulate matter, soot, and reaction product studies nitrated pyrenes [29] and benzo[a]pyrenes [30] have been reported since then. Other mass spectrometric methods for the analysis of nitro-PAH include HPLC-MS with a particle beam interface (EI and CI) [31], HPLC-GC systems coupled to an atomic emission detector or an ion trap mass spectrometer [32,33], and UV laser desorption and ionization coupled to negative ion time-of-flight mass spectrometry (TOF-MS) [34].…”

Section: Christian Schauer · Reinhard Niessner · Ulrich Pöschlmentioning

confidence: 99%

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Analysis of nitrated polycyclic aromatic hydrocarbons by liquid chromatography with fluorescence and mass spectrometry detection: air particulate matter, soot, and reaction product studies

Schauer

Pöschl

2004

Analytical and Bioanalytical Chemistry

100

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Polycyclic aromatic hydrocarbons (PAH) and their nitrated derivatives (nitro-PAH) are environmental pollutants which pose a threat to human health even at low concentration levels. In this study, efficient analytical methods for the analysis of nitro-PAH and PAH (extraction, clean-up, chromatographic separation, and spectrometric detection) have been developed, characterized, and applied to aerosol samples. The separation and quantification of 12 nitro-PAH was carried out by reversed-phase high performance liquid chromatography (HPLC), on-line reduction, and fluorescence detection. The detection limits were in the range of 0.03-0.5 microg L(-1) (6-100 pg in the investigated sample aliquots), and the recovery rates from soot samples were 70-90%. Nitro-PAH and PAH concentrations have been determined for different types of soot and for urban, rural, and alpine fine air particulate matter (PM2.5). For the first time, trace amounts of nitro-PAH have been detected in a high-alpine clean air environment. The on-line reduction and fluorescence technique has been complemented by atmospheric pressure chemical ionization time-of-flight mass spectrometry (APCI-TOF-MS). The MS detection allowed the analysis of partially nitrated and oxygenated PAH in laboratory studies of the heterogeneous reaction of PAH on soot and glass fiber substrates with gaseous nitrogen oxides and ozone. It led to the tentative identification of a previously unknown nitrated derivative of the particularly toxic PAH benzo[ a]pyrene (BaP-nitroquinone), and provides the first experimental evidence that PAH-nitroquinones can be formed by reaction of PAH with atmospheric photooxidants.

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Section: Resultssupporting

confidence: 85%

Section: Christian Schauer · Reinhard Niessner · Ulrich Pöschlmentioning

confidence: 99%

Analysis of nitrated polycyclic aromatic hydrocarbons by liquid chromatography with fluorescence and mass spectrometry detection: air particulate matter, soot, and reaction product studies

Schauer

Pöschl

2004

Analytical and Bioanalytical Chemistry

100

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“…The detection limit for 1-NP was 0.32 fmol/injection. This value is two orders of magnitude lower than the values obtained with recent HPLC methods with on-line reduction and fluorescence detection [21,22], the LC-atmospheric pressure chemical ionization (APCI)-MS method [23] and HPLC with electrochemical detection [24], and comparable…”

Section: Resultsmentioning

confidence: 62%

Determination of 1-nitropyrene in low volume ambient air samples by high-performance liquid chromatography with fluorescence detection

Ohno¹,

Toriba²,

Kameda³

et al. 2009

Journal of Chromatography A

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To measure the actual exposure of a person to 1-nitropyrene (1-NP) in airborne particulate matter, it is considered more accurate to collect air samples with a portable air sampler than to sample at a fixed location. However, because the portable samplers can sample only small volumes, a sensitive method is needed to analyze the compounds that are collected on a filter. Here we describe a high-performance liquid chromatographic (HPLC) method with fluorescence detection that is sensitive and precise enough for use with portable air samplers. The developed column-switching system successfully removed the interfering substances in the samples with only a simple pretreatment. To improve the precision of the measurement, deuterated 1-NP was used as an internal standard, and it eluted immediately prior to 1-NP with sufficient resolution (R s , 1.50).The detection limit was 0.32 fmol/injection, and the calibration range was from 2 to 100 fmol. The proposed method was applied to determining 1-NP in fine airborne particulate matter (PM 2.5 ) at two sites with low pollution levels. 1-NP was detected in all samples at concentrations in the low fmol/m 3 range. The proposed method has enough sensitivity and precision to determine 1-NP in the limited air volume of the portable sampler.

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“…Nitrated PAHs can be detected more easily than PAHs by LC/MS, because the nitro group has a high polarity. Simultaneous determination of 1-nitropyrene, 1,3-, 1,6-and 1,8-dinitropyrene by LC/APCI/MS has been reported by Moriwaki et al (2000) [41]. In the report, the [M] -peaks of nitrated PAHs were observed by APCI in negative ion mode, and soil samples were determined to contain nitrated pyrene at the ng/g level by this method.…”

Section: Discussion 21 Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 98%

Liquid Chromatographic- Mass Spectrometric Methods for the Analysis of Persistent Pollutants: Polycyclic Aromatic Hydrocarbons, Organochlorine Compounds and Perfluorinated Compounds

Moriwaki¹

2005

COC

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Liquid chromatography coupled with mass spectrometry (LC/MS) has become an invaluable technique for trace analysis of pollutants in the environment. Use of this technique offers a faster, more convenient, and more sensitive way to analyze many environmental pollutants. Of these, persistent pollutants in environment, some of which elicit toxicities, are monitored extensively in various environmental matrices as they are known to accumulate biologically through the food chain. Using LC/MS for the analysis of persistent pollutants has the potential to contribute to the simplification of analytical methods and the enhancement of sensitivity. This article presents a review of LC/MS methods published thus far on the determination of polycyclic aromatic hydrocarbons, organochlorine compounds and perfluorinated acids.

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Direct Determination of NO2-Substituted Pyrenes by Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Mass Spectrometry

Abstract: Polycyclic aromatic compounds (PACs) are widespread as environmental contaminants, and many of them have a high mutagenicity and carcinogenicity, suggesting that these compounds pose risks to human health.

Cited by 15 publications

References 8 publications

Analysis of nitrated polycyclic aromatic hydrocarbons by liquid chromatography with fluorescence and mass spectrometry detection: air particulate matter, soot, and reaction product studies

Analysis of nitrated polycyclic aromatic hydrocarbons by liquid chromatography with fluorescence and mass spectrometry detection: air particulate matter, soot, and reaction product studies

Determination of 1-nitropyrene in low volume ambient air samples by high-performance liquid chromatography with fluorescence detection

Liquid Chromatographic- Mass Spectrometric Methods for the Analysis of Persistent Pollutants: Polycyclic Aromatic Hydrocarbons, Organochlorine Compounds and Perfluorinated Compounds

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