Direct determination of praziquantel in pharmaceutical formulations and human plasma by cathodic adsorptive stripping differential-pulse voltammetry

Ghoneim, Mohammed M.; Mabrouk, Mokhtar M.; Tawfik, A

doi:10.1016/s0731-7085(02)00457-0

Cited by 14 publications

(9 citation statements)

References 19 publications

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“…This drug has been used for the treatment of schistosomiasis (bilharziasis) caused by all Schistosoma species pathogenic to humans because it is effective against all stages of Schistosoma infection including the acute phase and the chronic phase, which may associate with hepatosplenic involvement. In additional, it also has been used for the treatment of clonorchiasis caused by Clonorchis sinensis (Chinese liver fluke) and opisthorchiasis caused by Opisthorchis viverrini (liver fluke) [1]. For this reason, PZQ has become the drug of choice for the treatment and morbidity control of schistosomiasis, a disease that affects about 200 million people worldwide, leading to the loss of 1.53 million disability-adjusted life years [2].…”

Section: Introductionmentioning

confidence: 99%

Development and physicochemical characterization of solid dispersions containing praziquantel for the treatment of schistosomiasis

Dametto

Polese

et al. 2016

J Therm Anal Calorim

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Praziquantel (PZQ) is an anthelminthic agent active against parasitic flatworms of the Schistosoma type and the most important drug for the treatment and morbidity control of schistosomiasis. In this study, a highperformance liquid chromatography method was employed for quantification of PZQ in the physical mix (PM) and solid dispersion (SD) prepared by Fusion Method utilizing the carriers Gelucire Ò 50/13 and mannitol in ratio 3:1; 1:1; 1:3 PZQ/carrier. Furthermore, PM and SD were characterized by Thermogravimetry-Differential Thermal Analysis, Differential Scanning Calorimetry, Infrared Spectroscopy, X-Ray Diffraction, and Scanning Electron Microscopy. Solubility assay was made to evaluate the solubility of PZQ in purified water, in 0.1 mol L-1 HCl solution and 0.2 mol L-1 buffered phosphate solution. PZQ dissolution test was carried out in 0.1 mol L-1 HCl and 0.2 M buffered phosphate (pH 6.8). The interaction between PZQ and carriers in PMs and SDs was evidenced due to experimental enthalpy, which was different from expected enthalpy. However, this interaction did not affect the solubility of the drug. In PZQ dissolution test, the best result was for SD 1:1 PZQ/Gelucire, which presented higher dissolution rate and release extension than PM and PZQ. Thus, SD may be a strategy to enhance the solubility and dissolution, a crucial step in the development of a pharmaceutical dosage form containing PZQ for possible application in the treatment of schistosomiasis.

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Section: Introductionmentioning

confidence: 99%

Development and physicochemical characterization of solid dispersions containing praziquantel for the treatment of schistosomiasis

Dametto

Polese

et al. 2016

J Therm Anal Calorim

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“…Many analytical methods have been described in the literature for the determination of PZQ and PP alone or in combination with other drugs using various detection techniques, such as potentiometric titration [2], voltammetry [3,4], spectrophotometry [5][6][7]. Literature review states that few RP-HPLC methods [8][9][10][11][12][13] and nuclear magnetic resonance method [14], high-performance liquid chromatography-tandem mass spectrometry methods [15,16] have been reported for the estimation of praziquantel individually and with other drug combinations.…”

Section: Introductionmentioning

confidence: 99%

A Validated Rp-HPLC Method for Simultaneous Estimation of Pyrantel Pamoate and Praziquantel in Bulk and Pharmaceutical Dosage Form

Rajesh

James

2019

Int J Pharm Pharm Sci

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Objective: To develop a simple, accurate and precise reverse-phase high-performance liquid chromatography (RP-HPLC) method and subsequently validate for the simultaneous estimation of praziquantel (PZQ) and pyrantel pamoate (PP) in the pharmaceutical dosage form. Methods: The chromatographic separation was achieved on Phenomenex Luna C18 column (250 mm × 4.6 mm, 5 μm) as stationary phase maintained at an ambient temperature with a mobile phase comprising of water: acetonitrile (20: 80) at a flow rate of 1.0 ml/min and UV detection at 220 nm. Results: The retention time of PZQ and PP was found to be 3.897 min and 1.697 min respectively. The method was validated in terms of specificity, accuracy, precision, linearity and robustness as per ICH guidelines. Linearity was obtained in the concentration range of 20–60 μg/ml for both PZQ and PP with correlation coefficients of 0.987 and 0.998 respectively. The accuracy of the method was determined using a recovery test and found as 98.44 % to 100.35 %. All parameters are found to be within the acceptable limit. Conclusion: The developed RP-HPLC method was simple, rapid, accurate, precise for the simultaneous estimation of PZQ and PP in bulk and tablet dosage form.

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“…Since the antihelminthic spectra of most drugs used for treatment is limited, combinations of more than one active ingredient are required to control helminthic infections 2 .In this context the mix of two drugs such as Fenbendazole, methyl N-(6-phenylsulfanyl-1H-benzoimidazol-2-yl) carbamate, (FBZ) (Fig.1a), this drug is thought to bind to tubulin and thereby preventing its polymerization to form microtubules, but it also inhibits fumarat reductase and glucose transport. As a result, parasites may die of starvation 3 and Praziquantel, (RS)-2-(Cyclohexyl-carbonyl)-1,2,3,6,7,11,b-hexahydro-4H-pyrazino (2,1-alpha) isoquinoline-4-one, (PZQ) (Fig.1b), is a synthetic antihelminthic agent, used against many cestodes, and for the treatment of schistosomiasis, as well other fluke infections pathogenic to humans 4,5 , are commonly used in domestic animals for the prevention and control of a wide variety of parasitic diseased, in a pharmaceutical combination with a 1:10 mass ratio.…”

Section: Introductionmentioning

confidence: 99%

“…The evaluation of the veterinary preparations require of the determination of their active ingredients in different samples such as drug formulations and biological matrices. Numerous methods for the quantification of either one or a mixture of FBZ and PZQ with others compounds has been reported in the literature, in biological matrices using techniques such as, liquid membrane and solid phase extraction with liquid chromatography electrospray mass spectrometry (LC ES MS) 7 liquid membrane extraction and liquid chromatography (LC) 8 , high performance liquid chromatography (HPLC) 9 , LC 10,11 polymer solid phase extraction coupled to square wave voltammetry at carbon fibre microelectrodes 12 , cathodic adsorptive stripping differential pulse voltammetry (CASDPV) 13 . In feeds and pharmaceutical formulations by techniques such as, CASDPV 13 , colorimetric method 14 , second derivative spectrophotometry and multivariated calibration methods 15 , amperometric detection with flow injection 16 , HPLC capillary electrophoresis 17 , HPLC 18,19 , gas liquid chromatography (GLC) 20 , densitometric determination 21 , voltammetric determination with poly(3-methylthiophene)-modified glassy carbon electrode 22 , voltammetric determination 16,23 , voltametric stripping methods 24 and polarographic assay 25 .…”

Section: Introductionmentioning

confidence: 99%

Co-Determination of Two Antiparasitics Drugs by Derivative Spectrophotometry and Its Photodegradation Studies

Soto¹,

Otipka

Contreras³

et al. 2013

J. Chil. Chem. Soc.

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This work proposes a method for the co-determination of praziquantel and fenbendazole in veterinary pharmaceutical formulations (VPF) by second-derivative spectrophotometry at 226.4 nm (Zero-crossing method) and 324.6 nm (Graphic method), respectively. The excipients commonly used in VPF do not interfere. To obtain the optimal determination conditions, studies of solvent and light effects, were performed. The results of the light effects show that the expose of both drugs to indirect light and darkness does not produce any chemical change, since their zero-order spectra remained unchanged during the experiment. However, the exposition to the direct light only affects the fenbendazole. The zero order spectrum of FBZ in EtOH-HCl, present two broad bands (200 to 240 nm and 260 to 330 nm), these signals disappear and appear others totally different, at 230 nm and other with two peaks (285 and 295 nm). The FT-IR spectrum shows characteristics signals of the FBZ structure as the amide-NH group (3336.3 cm -1 ), this signal disappear indicating the bond break of the amide group caused by the exposition to sunlight for a time. These spectral changes evidenced a structural transformation as a result of photochemical decomposition in acid medium.

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Direct determination of praziquantel in pharmaceutical formulations and human plasma by cathodic adsorptive stripping differential-pulse voltammetry

Cited by 14 publications

References 19 publications

Development and physicochemical characterization of solid dispersions containing praziquantel for the treatment of schistosomiasis

Development and physicochemical characterization of solid dispersions containing praziquantel for the treatment of schistosomiasis

A Validated Rp-HPLC Method for Simultaneous Estimation of Pyrantel Pamoate and Praziquantel in Bulk and Pharmaceutical Dosage Form

Co-Determination of Two Antiparasitics Drugs by Derivative Spectrophotometry and Its Photodegradation Studies

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