Direct electrochemistry and electrocatalysis of horseradish peroxidase with hyaluronic acid–ionic liquid–cadmium sulfide nanorod composite material

A review on electrodes modified with ionic liquids

“…In particular, IL containing film enables to observe direct electrochemistry of horseradish peroxidase [27,32,77,78,92,94,100,101,137], hemoglobin [76,80,85,86,90,91,97,99,103,113,115,128,136,[159][160][161], myoglobin [83,89,98,113,127,162], cytochrome c [93,104,113,116], catalase [109] and chloroperoxidase [81]. These and other electrodes are capable of mediatorless bioelectrocatalysis of H 2 O 2 electroreduction with immobilized enzymes as horseradish peroxidase [27,32,78,92,95,96,[100][101][102]137], hemoglobin [76,80,…”

“…These and other electrodes are capable of mediatorless bioelectrocatalysis of H 2 O 2 electroreduction with immobilized enzymes as horseradish peroxidase [27,32,78,92,95,96,[100][101][102]137], hemoglobin [76,80,85,90,97,99,103,128,136,[159][160][161], cytochrome c [93,104], myoglobin [83,89,98,127], catalase [109] or chloroperoxidase [81]. Other group of IL containing composite film electrodes modified with horseradish peroxidase [27], hemoglobin [80,86,90,91,128], cytochrome c [116] or chloroperoxidase [81] exhibits catalysis of dioxygen reduction.…”

“…These components contribute to mechanical and electrical properties of the film, and many of them as conductive nanotubes or nanoparticles provide electrocatalytic activity. Combinations of polymer, conductive nanoparticles and IL [126,127], polymer and nonconductive nanoparticles [128,129], polymer, carbon nanotubes or nanofibers and IL [34,[130][131][132][133][134][135] and polymer, IL and other particles as CuS microspheres [136] or CdS nanorods [137] are the examples. The preparation of film from surfactant, MWCNTs and IL, has to be noted [79].…”

Direct electrochemistry of horseradish peroxidase immobilized on electrografted 4-ethynylphenyl film via click chemistry

“…The surface concentration of HRP ( ) is about 4.4 × 10 −11 mol cm −2 , which is close to the theoretical monolayer coverage (about 2 × 10 −11 mol cm −2 ) [36], indicating that a monolayer of immobilized HRP participated in the electron transportation process. According to Laviron [37], the heterogeneous electron transfer rate constant k s is calculated to be 2.13 s −1 , which is larger than the value 1.15 s −1 obtained by immobilizing HRP on Zinc oxide nanorods [9], 0.92 s −1 for HRP immobilized on hexagonal mesoporous silica matrix [38], and 0.65 s −1 for HRP immobilized on composite material consisted of hyaluronic acid, ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate and cadmium sulfide nanorod [39]. It suggests a relatively fast ET, which may be caused by the high electron transfer rate through the triazole linkage and benzene ring [32].…”

Graphite Furnace Atomic Absorption Spectrophotometric Determination of Trace Horseradish Peroxidase Using Nanosilver

“…[6][7][8][9][10][11][12] Thus, its detection is very important. Presently, the main assays for detection of HRP activity including voltammetry, 12 spectrophotometry, 13-15 fluorescence spectrometry, 16 chemiluminescence, 17 flow injection analysis, 18 resonance scattering methods.…”