Direct electrochemistry of cytochrome c on a multi-walled carbon nanotubes modified electrode and its electrocatalytic activity for the reduction of H2O2

Zhao, Guang‐Chao; Yin, Zheng‐Zhi; Zhang, Li; Wei, Xian‐Wen

doi:10.1016/j.elecom.2005.01.006

Cited by 216 publications

(76 citation statements)

References 32 publications

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“…electron transfer reaction of some important biomolecules, such as cytochrome c [9][10][11], myoglobin [12][13][14], glucose [15][16][17][18][19], catalase [20,21], NADH [22][23][24][25][26], and haemoglobin [27,28], among others.…”

Section: E-mail Address: Brett@ciucpt (Cma Brett)mentioning

confidence: 99%

Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol

Gouveia‐Caridade

Pauliukaitė

Brett

2008

Electrochimica Acta

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Functionalised multi-walled carbon nanotubes (MWCNTs) were cast on glassy carbon (GC) and carbon film electrodes (CFE), and were characterised electrochemically and applied in a glucose-oxidase-based biosensor. MWCNT-modified carbon film electrodes were then used to develop an alcohol oxidase (AlcOx) biosensor, in which AlcOx-BSA was cross-linked with glutaraldehyde and attached by drop-coating. The experimental conditions, applied potential and pH, for ethanol monitoring were optimised, and ethanol was determined amperometrically at −0.3 V vs. SCE at pH 7.5. Electrocatalytic effects of MWCNT were observed with respect to unmodified carbon film electrodes. The sensitivity obtained was 20 times higher at carbon film/MWCNT-based biosensors than without MWCNT.

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Section: E-mail Address: Brett@ciucpt (Cma Brett)mentioning

confidence: 99%

Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol

Gouveia‐Caridade

Pauliukaitė

Brett

2008

Electrochimica Acta

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“…It is known that Cyt c adsorbs strongly on conventional metal electrodes like Pt, Hg, Au or Ag 17 and conformational changes suffered by the protein (unfolding and/or denaturation) lead to slow electrontransfer kinetics 18,19 . This difficulty has been overcome by using electrode modifiers such as metal oxides, advanced carbon materials, DNA or lipid membranes [20][21][22][23][24][25][26] . In particular, the modification of surfaces with organic thin films like self-assembled monolayers (SAM), has been extensively used to manipulate the electrode surface, promoting the appropriate orientation of the protein and thus enhancing its electroactivity [27][28][29][30][31][32][33][34][35][36] .…”

Section: Introductionmentioning

confidence: 99%

Direct Electron Transfer to Cytochrome c Induced by a Conducting Polymer

et al. 2017

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The direct electron transfer (DET) to redox proteins has become a central topic in the development of biotechnological devices. The present work explores the mechanisms of the direct electrochemistry between cytochrome c and a conducting polymer, PEDOT-PSS. This polymer has been electrosynthesized from its monomers in aqueous solution on gold electrodes and its capabilities for DET to cyt c have been examined by electrochemical and spectroelectrochemical methods. The polymer was electrodeposited with controlled thickness and we determined the electron transfer rate constant for cyt c oxidation was about 2 orders of magnitude higher than those obtained at conventional electrodes. Spectroelectrochemical measurements allowed to evaluate the redox state of the polymer as a function of the potential and, in addition, the observation of intrinsic cyt c redox activity upon electron transfer from the conducting polymer. During the oxidation process of this protein, lysine residues placed near the heme crevice interact electrostatically with the anionic polyelectrolyte PSS. This interaction favors the orientation of the heme group towards the chains of PEDOT backbone, which is the eventual responsible for the electron transfer to the protein.3

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“…However, current scientific attention focuses on the development of the so-called third generation of biosensors. In such devices, the direct electron transfer take place between enzyme molecules and electrode surfaces with no redox mediation [11][12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

Modulation of the Silica Sol–Gel Composition for the Promotion of Direct Electron Transfer to Encapsulated Cytochrome c

et al. 2014

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The direct electron transfer between indium-tin oxide electrodes (ITO) and cytochrome c encapsulated in different sol-gel silica networks was studied. Cyt c@silica modified electrodes were synthesized by a two-step encapsulation method mixing a phosphate buffer solution with dissolved cytochrome c and a silica sol prepared by the alcohol free sol-gel route. These modified electrodes were characterized by cyclic voltammetry, UV-visible spectroscopy and in situ UV-visible spectroelectrochemistry.The electrochemical response of encapsulated protein is influenced by the terminal groups of the silica pores. Cyt c does not present electrochemical response in conventional silica (hydroxyl terminated) or phenyl terminated silica. Direct electron transfer to encapsulated cytochrome c and ITO electrodes only takes place when the protein is encapsulated in methyl modified silica networks.

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Direct electrochemistry of cytochrome c on a multi-walled carbon nanotubes modified electrode and its electrocatalytic activity for the reduction of H2O2

Cited by 216 publications

References 32 publications

Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol

Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol

Direct Electron Transfer to Cytochrome c Induced by a Conducting Polymer

Modulation of the Silica Sol–Gel Composition for the Promotion of Direct Electron Transfer to Encapsulated Cytochrome c

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