Direct electrochemistry of hemoglobin and its electrocatalytic effect based on its direct immobilization on carbon ionic liquid electrode

Safavi, Afsaneh; Maleki, N.; Moradlou, Omran; Sorouri, Mohsen

doi:10.1016/j.elecom.2007.12.026

Cited by 128 publications

(45 citation statements)

References 27 publications

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“…18 Enzymes are usually active and protein refolding is improved in ILs. [19][20][21][22][23][24][25] Immobilization of redox proteins is significant step in constructing biosensors or bioreactors. 26 Biocompatible materials have been used widely by entrapment or encapsulation of proteins within them to realize direct electrochemistry of redox proteins for their desirable properties, such as nontoxic and biocompatible, and potential applications for the fabrication of biosensors.…”

Section: Introductionmentioning

confidence: 99%

Direct Electrochemistry and Electrocatalysis Behaviors of Glucose Oxidase Based on Hyaluronic Acid‐Carbon Nanotubes‐Ionic Liquid Composite Film

et al. 2010

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Multi-walled carbon nanotubes (MWNTs) were dispersed in the ionic liquid [BMIM] [BF 4 ]-MWNTs could obviously improve the diffusion of ferricyanide toward the electrode surface. The experimental results of CV showed that a pair of well-defined and quasi-reversible peaks of GOx at the modified electrode was exhibited, and the redox reaction of GOx at the modified electrode was surface-confined and quasi-reversible electrochemical process. The average surface coverage of GOx and the apparent Michaelis-Menten constant were 8.5×10-9 mol/cm 2 and 9.8 mmol/L, respectively. The cathodic peak current of GOx and the glucose concentration showed linear relationship in the range from 0.1 to 2.0 mmol/L with a detection limit of 0.03 mmol/L (S/N＝3). As a result, the method presented here could be easily extended to immobilize and obtain the direct electrochemistry of other redox enzymes or proteins.

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Section: Introductionmentioning

confidence: 99%

Direct Electrochemistry and Electrocatalysis Behaviors of Glucose Oxidase Based on Hyaluronic Acid‐Carbon Nanotubes‐Ionic Liquid Composite Film

et al. 2010

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“…CILE was a new kind of modified electrode with many specific advantages such as high conductivity, provision of excellent electron transfer ability and good anti-fouling properties. A thin layer of IL cationic ions were present on the surface of CILE, 5,[18][19][20] which was also considered to be able to play an inherent catalytic ability toward the electrochemical reaction of Hb. At the same time CTS was a biocompatible biomacromolecules and often used as a immobilization material to fix the biomolecules on the surface of biosensors.…”

Section: Resultsmentioning

confidence: 99%

Electrochemistry of Hemoglobin in the Chitosan and TiO₂Nanoparticles Composite Film Modified Carbon Ionic Liquid Electrode and Its Electrocatalysis

Sun¹,

Li²,

Liu

et al. 2009

Bulletin of the Korean Chemical Society

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Direct electron transfer of hemoglobin (Hb) in the chitosan (CTS) and TiO2 nanoparticles (nano-TiO2) composite films was achieved by using a room temperature ionic liquid of 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF6) modified carbon paste electrode (CILE) as the basal electrode. UV-Vis and FT-IR spectroscopy indicated that Hb in the film retained the native structure. Electrochemical investigation indicated that a pair of well-defined quasi-reversible redox peaks of Hb heme Fe(III)/Fe(II) was obtained with the formal potential located at -0.340 V (νs. SCE) in pH 7.0 phosphate buffer solution (PBS). The electrochemical parameters such as the electron transfer coefficient (α), the electron transfer number (n) and the standard electron transfer rate constant (ks) were got as 0.422, 0.93 and 0.117 s -1 , respectively. The fabricated CTS/nano-TiO2/Hb/CILE showed good electrocatalytic ability to the reduction of trichloroacetic acid (TCA) and hydrogen peroxide (H2O2), which exhibited a potential application in fabricating a new kind of third generation biosensor.

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“…1 12 For example, the electrocatalytic reduction of hydrogen peroxide, 2 6,8 12 oxygen, 1,2 trichloroacetic acid, 7,12 and nitric oxide 2,12 …”

Section: Electrocatalytic Activity Of Hb-cf For O 2 Reduction and Itsmentioning

confidence: 99%

Bioelectrocatalytic Reduction of Oxygen by Hemoglobin-Adsorbed Carbon-Felt, and its Inhibition by Azide

Hasebe

Wang

2012

Electrochemistry

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Hemoglobin (Hb) was adsorbed onto a carbon-felt (CF), which is a microelectrode ensemble of microcarbon fiber (ca. 7 µm diameter) and possesses a random three-dimensional structure. Direct electron transfer between Hb heme and the CF was achieved without any electron mediating species and special materials. The Hb-adsorbed CF (Hb-CF) showed a pair of well-defined cyclic voltammetric peaks with the formal potential of −0.221 V (vs. Ag/AgCl) at pH 5.0 (0.1 M phosphate/citrate buffer), which is attributed to Hb Fe(III)/Fe(II) redox couple. The apparent heterogeneous electron-transfer rate constant (k s ) was estimated to be 14.6 s −1 . Furthermore, the Hb-CF exhibited an excellent bioelectrocatalytic activity for the reduction of O 2 . This bioelectrocatalytic activity was inhibited by azide, which binds to active heme center of Hb.

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Direct electrochemistry of hemoglobin and its electrocatalytic effect based on its direct immobilization on carbon ionic liquid electrode

Cited by 128 publications

References 27 publications

Direct Electrochemistry and Electrocatalysis Behaviors of Glucose Oxidase Based on Hyaluronic Acid‐Carbon Nanotubes‐Ionic Liquid Composite Film

Direct Electrochemistry and Electrocatalysis Behaviors of Glucose Oxidase Based on Hyaluronic Acid‐Carbon Nanotubes‐Ionic Liquid Composite Film

Electrochemistry of Hemoglobin in the Chitosan and TiO₂Nanoparticles Composite Film Modified Carbon Ionic Liquid Electrode and Its Electrocatalysis

Bioelectrocatalytic Reduction of Oxygen by Hemoglobin-Adsorbed Carbon-Felt, and its Inhibition by Azide

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Direct electrochemistry of hemoglobin and its electrocatalytic effect based on its direct immobilization on carbon ionic liquid electrode

Cited by 128 publications

References 27 publications

Direct Electrochemistry and Electrocatalysis Behaviors of Glucose Oxidase Based on Hyaluronic Acid‐Carbon Nanotubes‐Ionic Liquid Composite Film

Direct Electrochemistry and Electrocatalysis Behaviors of Glucose Oxidase Based on Hyaluronic Acid‐Carbon Nanotubes‐Ionic Liquid Composite Film

Electrochemistry of Hemoglobin in the Chitosan and TiO2Nanoparticles Composite Film Modified Carbon Ionic Liquid Electrode and Its Electrocatalysis

Bioelectrocatalytic Reduction of Oxygen by Hemoglobin-Adsorbed Carbon-Felt, and its Inhibition by Azide

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Electrochemistry of Hemoglobin in the Chitosan and TiO₂Nanoparticles Composite Film Modified Carbon Ionic Liquid Electrode and Its Electrocatalysis