Direct Electrochemistry of Myoglobin on a Room Temperature Ionic Liquid Modified Electrode and Its Application to Nitric Oxide Biosensing

Abstract

Myoglobin (Myb) was successfully immobilized on a room temperature ionic liquid (RTIL), 1-ethyl-3-methyl imidazolium tetrafluoroborate ([EMIM][BF 4 ]) modified basal plane graphite (BPG) electrode. The electrochemical behavior of Myb on RTIL modified BPG electrode was explored and the results from cyclic voltammetry (CV) showed a well-defined and quasi-reversible CV peaks with a formal potential of À 0.379 V (versus Ag/AgCl) in a phosphate buffer solution (pH 7.0). RTIL shows an obvious promotion for the direct electron-transfer between Myb and BPG electrode. Myb adsorbed on electrode surface exhibits an obvious electrocatalytic activity for the reduction of nitric oxide (NO). The catalytic current is corresponding linearly to the NO concentration in the range of 7.0 Â 10 À7 to 7.0 Â 10 À6 M with a limit of detection of 2.0 Â 10 À7 M (three times the ratio of signal to noise, S/N ¼ 3).

A review on electrodes modified with ionic liquids

“…In particular, IL containing film enables to observe direct electrochemistry of horseradish peroxidase [27,32,77,78,92,94,100,101,137], hemoglobin [76,80,85,86,90,91,97,99,103,113,115,128,136,[159][160][161], myoglobin [83,89,98,113,127,162], cytochrome c [93,104,113,116], catalase [109] and chloroperoxidase [81]. These and other electrodes are capable of mediatorless bioelectrocatalysis of H 2 O 2 electroreduction with immobilized enzymes as horseradish peroxidase [27,32,78,92,95,96,[100][101][102]137], hemoglobin [76,80,…”

Electrochemistry of myoglobin in Nafion and multi-walled carbon nanotubes modified carbon ionic liquid electrode

“…Zhang et al studied the direct electrochemistry of Mb on an ionic liquid modified electrode and its application to nitric oxide biosensing [28]. Lu et al investigated the direct electrochemistry of HRP in a composite system based on chitosan and 1-butyl-3-methylimidazolium tetrafluoroborate [29].…”

Highly Sensitive Nitric Oxide Sensing Using Three‐Dimensional Graphene/Ionic Liquid Nanocomposite

“…Recently, functional nanomaterials have opened up a window of opportunity in electrochemistry due to their high surface area-tovolume ratio and tunable electron transport which promote efficient electron transfer [22][23][24]. Nanomaterials such as single-walled carbon nanotubes (SWNTs) [25], multi-walled carbon nanotubes (MWNTs) [26][27][28], ionic liquid [29] and gold nanoparticles (Au NPs) [30][31][32] have been widely used in electrochemical NO sensors to enhance sensitivities and push down detection limits. However, the real time detection of NO remains a challenge due to the trace level of the endogenous NO, and more critically, the rapid reaction of NO with oxygen and its short half-life of 5 seconds [13,33,34].…”