Direct electrochemistry of sarcosine oxidase on graphene, chitosan and silver nanoparticles modified glassy carbon electrode and its biosensing for hydrogen peroxide

Zhou, Yunlei; Yin, Huanshun; Meng, Xiaomeng; Xu, Zigang; Fu, Yan-Rong; Ai, Shiyun

doi:10.1016/j.electacta.2012.04.014

Cited by 65 publications

(29 citation statements)

References 38 publications

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“…33 Figure 4 illustrates the typical Nyquist diagrams obtained on different modified electrodes in 1.0 mM [Fe(CN) 6 ] 3−/4− prepared in 0.1 M KCl and the corresponding electrical equivalent circuit (inset). In this circuit, the parameter R s represents the solution resistance and R ct is the charge-transfer resistance, which reflects the counterion transfer happening at the film− electrolyte interface.…”

Section: Characterization On the Biosensor Fabricationmentioning

confidence: 99%

Construction of a Novel Colitoxin DNA Biosensor Based on Cross-Linker-Free Fixation of Probe Fragments on the Interface of Rugby-Ball-Shaped CoS₂ Submicroparticles and Poly(2-thiophenesulfonyl chloride) Composite Film

Chen

Wang

et al. 2015

Ind. Eng. Chem. Res.

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Rugby-ball-shaped CoS 2 (rCoS 2 ) submicroparticles have been synthesized via a simple hydrothermal reaction. The rCoS 2 submicroparticles were then casted on a glassy carbon electrode and served as a highly conductive and large surface area platform for the electrosynthesis of poly(2-thiophenesulfonyl chloride) (pTSC), using 2-thiophenesulfonyl chloride as the monomer. The stepwise modification process was characterized by attenuated total reflectance−Fourier transform infrared spectroscopy (ATR-FTIR) and different electrochemical technologies. The stable pTSC layer was utilized as a functional interface to immobilize amino-modified probe DNA via a facile sulfonyl−amino condensation reaction, without the assistance of any other cross-linkers. Because of the synergistic effect of rCoS 2 and pTSC from the hybrid material, the developed DNA biosensor could detect target DNA over a wide dynamic range, from 1.0 × 10 −13 to 1.0 × 10 −7 M, with the detection limit down to 1.1 × 10 −14 M. Benefiting from the steady electropolymerization film of pTSC and the stable sulfamide bond between the amino-modified DNA and pTSC, the biosensor also showed high stability, reusability, and reproducibility.

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Section: Characterization On the Biosensor Fabricationmentioning

confidence: 99%

Construction of a Novel Colitoxin DNA Biosensor Based on Cross-Linker-Free Fixation of Probe Fragments on the Interface of Rugby-Ball-Shaped CoS₂ Submicroparticles and Poly(2-thiophenesulfonyl chloride) Composite Film

Chen

Wang

et al. 2015

Ind. Eng. Chem. Res.

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“…The attachment of graphene to a supporting electrode can be achieved through simple electrode treatments, such as the application of graphene suspensions to GC, which promotes the formation of a stable thin film owing to π–π stacking interactions . Alternatively, composite mixtures of chitosan and graphene can be applied to carbon electrode surfaces as thin films which promote the physisorption of redox proteins . A review of the uses of graphene in electrochemical sensors and biosensors has been compiled by Shao et al …”

Section: Carbon Electrodesmentioning

confidence: 99%

Methodologies for “Wiring” Redox Proteins/Enzymes to Electrode Surfaces

Yates

Fascione

Parkin

2018

Chemistry A European J

109

116

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The immobilization of redox proteins or enzymes onto conductive surfaces has application in the analysis of biological processes, the fabrication of biosensors, and in the development of green technologies and biochemical synthetic approaches. This review evaluates the methods through which redox proteins can be attached to electrode surfaces in a “wired” configuration, that is, one that facilitates direct electron transfer. The feasibility of simple electroactive adsorption onto a range of electrode surfaces is illustrated, with a highlight on the recent advances that have been achieved in biotechnological device construction using carbon materials and metal oxides. The covalent crosslinking strategies commonly used for the modification and biofunctionalization of electrode surfaces are also evaluated. Recent innovations in harnessing chemical biology methods for electrically wiring redox biology to surfaces are emphasized.

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“…In context Zhou et al. needed 118 nmole/cm 2 SOx at an electrode. They achieved this by producing a high surface area for immobilisation with a graphene, chitosan and silver nanoparticle (AgNPs) modified glassy carbon electrode.…”

Section: Introductionmentioning

confidence: 99%

A Biosilification Fusion Protein for a ‘Self‐immobilising’ Sarcosine Oxidase Amperometric Enzyme Biosensor

Chen

Hall

2020

Electroanalysis

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Monomeric sarcosine oxidase (mSOx) fusion with the silaffin peptide, R5, designed previously for easy protein production in low resource areas, was used in a biosilification process to form an enzyme layer electrode biosensor. mSOx is a low activity enzyme (10–20 U/mg) requiring high amounts of enzyme to obtain an amperometric biosensor signal, in the clinically useful range <1 mM sarcosine, especially since the Km is >10 mM. An amperometric biosensor model was fitted to experimental data to investigate dynamic range. mSOx constructs were designed with 6H (6×histidine) and R5 (silaffin) peptide tags and compared with native mSOx. Glutaraldehyde (GA) cross‐linked proteins retained ∼5 % activity for mSOx and mSOx‐6H and only 0.5 % for mSOx‐R5. In contrast R5 catalysed biosilification on (3‐mercaptopropyl) trimethoxysilane (MPTMS) and tetramethyl orthosilicate (TMOS) particles created a ‘self‐immobilisation’ matrix retaining 40 % and 76 % activity respectively. The TMOS matrix produced a thick layer (>500 μm) on a glassy carbon electrode with a mediated current due to sarcosine in the clinical range for sarcosinemia (0–1 mM). The mSOx‐R5 fusion protein was also used to catalyse biosilification in the presence of creatinase and creatininase, entrapping all three enzymes. A mediated GC enzyme linked current was obtained with dynamic range available for creatinine determination of 0.1–2 mM for an enzyme layer ∼800 nm.

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Direct electrochemistry of sarcosine oxidase on graphene, chitosan and silver nanoparticles modified glassy carbon electrode and its biosensing for hydrogen peroxide

Cited by 65 publications

References 38 publications

Construction of a Novel Colitoxin DNA Biosensor Based on Cross-Linker-Free Fixation of Probe Fragments on the Interface of Rugby-Ball-Shaped CoS₂ Submicroparticles and Poly(2-thiophenesulfonyl chloride) Composite Film

Construction of a Novel Colitoxin DNA Biosensor Based on Cross-Linker-Free Fixation of Probe Fragments on the Interface of Rugby-Ball-Shaped CoS₂ Submicroparticles and Poly(2-thiophenesulfonyl chloride) Composite Film

Methodologies for “Wiring” Redox Proteins/Enzymes to Electrode Surfaces

A Biosilification Fusion Protein for a ‘Self‐immobilising’ Sarcosine Oxidase Amperometric Enzyme Biosensor

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Direct electrochemistry of sarcosine oxidase on graphene, chitosan and silver nanoparticles modified glassy carbon electrode and its biosensing for hydrogen peroxide

Cited by 65 publications

References 38 publications

Construction of a Novel Colitoxin DNA Biosensor Based on Cross-Linker-Free Fixation of Probe Fragments on the Interface of Rugby-Ball-Shaped CoS2 Submicroparticles and Poly(2-thiophenesulfonyl chloride) Composite Film

Construction of a Novel Colitoxin DNA Biosensor Based on Cross-Linker-Free Fixation of Probe Fragments on the Interface of Rugby-Ball-Shaped CoS2 Submicroparticles and Poly(2-thiophenesulfonyl chloride) Composite Film

Methodologies for “Wiring” Redox Proteins/Enzymes to Electrode Surfaces

A Biosilification Fusion Protein for a ‘Self‐immobilising’ Sarcosine Oxidase Amperometric Enzyme Biosensor

Contact Info

Product

Resources

About

Construction of a Novel Colitoxin DNA Biosensor Based on Cross-Linker-Free Fixation of Probe Fragments on the Interface of Rugby-Ball-Shaped CoS₂ Submicroparticles and Poly(2-thiophenesulfonyl chloride) Composite Film

Construction of a Novel Colitoxin DNA Biosensor Based on Cross-Linker-Free Fixation of Probe Fragments on the Interface of Rugby-Ball-Shaped CoS₂ Submicroparticles and Poly(2-thiophenesulfonyl chloride) Composite Film