Direct electron transfer of horseradish peroxidase and its electrocatalysis based on carbon nanotube/thionine/gold composites

Wang, Zhijuan; Li, Meiye; Su, Pingping; Zhang, Yuanjian; Shen, Yanfei; Han, Dongxue; Ivaska, Ari; Niu, Li

doi:10.1016/j.elecom.2007.12.011

Cited by 63 publications

(28 citation statements)

References 34 publications

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“…Similar results have been reported for catalase covalently bound to electrospun nanofiber meshes filled with carbon nanotubes [40]. It was also reported that the conducting carbon nanotubes were able to promote direct electron transport from redox enzymes such as ascorbate oxidase, peroxidase, and Lac [38,[40][41][42][43][44][45][46]. Therefore, PpPD/Fe3O4 nanocomposite may also be suitable for enzyme immobilization, especially for redox enzymes.…”

Section: Removal Of Rb-19supporting

confidence: 68%

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

et al. 2016

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Magnetic poly(p-phenylenediamine) (PpPD) nanocomposite was synthesized via mixing p-phenylenediamine solution and Fe 3 O 4 nanoparticles and used as a carrier for immobilized enzymes. Successful synthesis of PpPD/Fe 3 O 4 nanofiber was confirmed by transmission electron microscopy and Fourier transform infrared spectroscopy. Laccase (Lac) was immobilized on the surface of PpPD/Fe 3 O 4 nanofiber through covalent bonding for reactive blue 19 dye removal. The immobilized Lac-nanofiber conjugates could be recovered from the reaction solution using a magnet. The optimum reaction pH and temperature for the immobilized Lac were 3.5 and 65 • C, respectively. The storage, operational stability, and thermal stability of the immobilized Lac were higher than those of its free counterpart. The dye removal efficiency of immobilized Lac was about 80% in the first 1 h of incubation, while that of free Lac was about 20%. It was found that the unique electronic properties of PpPD might underlie the high dye removal efficiency of immobilized Lac. Over a period of repeated operation, the dye removal efficiency was above 90% during the first two cycles and remained at about 43% after eight cycles. Immobilized Lac on PpPD/Fe 3 O 4 nanofiber showed high stability, easy recovery, reuse capabilities, and a high removal efficiency for reactive blue 19 dye; therefore, it provides an optional tool for dye removal from wastewater.

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Section: Removal Of Rb-19supporting

confidence: 68%

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

et al. 2016

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“…The consequent glucose biosensor fabricated by modifying a TiO 2 /CNT/Pt electrode with glucose oxidase yielded a high sensitivity with a response time of less than 3 s and a detection limit of 6 μmol·L −1 . Also, Wang et al, have used HRP incorporated into a multi-wall CNT/thionine/Au (MTAu) composite film via electrostatic interactions between positively charged HRP and negatively charged MTAu [190]. The resulting composite was able to retain the electrocatalytic activity of HRP and showed good direct electron transfer behavior.…”

Section: Recent Developmentsmentioning

confidence: 99%

“…In order to perform mechanistic studies of the immobilized proteins or to fabricate electrochemical biosensors, various nanocomposites of CNT with MnO 2 [186], NiO [187], TiO 2 [188], Pt [189], or Au [148,190] have also been prepared. Chen et al developed an amperometric glucose biosensor based on a MnO 2 /multi-wall CNT electrode [186].…”

Section: Recent Developmentsmentioning

confidence: 99%

Recent applications of carbon-based nanomaterials in analytical chemistry: Critical review

Scida

Stege

Haby

et al. 2011

Analytica Chimica Acta

398

168

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The objective of this review is to provide a broad overview of the advantages and limitations of carbon-based nanomaterials with respect to analytical chemistry. Aiming to illustrate the impact of nanomaterials on the development of novel analytical applications, developments reported in the 2005–2010 period have been included and divided into sample preparation, separation, and detection. Within each section, fullerenes, carbon nanotubes, graphene, and composite materials will be addressed specifically. Although only briefly discussed, included is a section highlighting nanomaterials with interesting catalytic properties that can be used in the design of future devices for analytical chemistry.

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“…Various hybrid nanomaterials films such as TiO 2 /MWNTs [23], Au NPs/CNTs [24,25], and MWNTsFc [26] have been used to immobilize proteins or enzymes on electrode surface for either mechanistic study of the proteins or fabricating electrochemical biosensors. These hybrid nanomaterials exhibited desirable microenvironment for protein immobilization and great facilitation of the electron transfer compared with those of the individual components [27,28].…”

Section: Introductionmentioning

confidence: 99%

Direct electrochemistry of myoglobin immobilized in NiO/MWNTs hybrid nanocomposite for electrocatalytic detection of hydrogen peroxide

et al. 2010

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A novel matrix, multiwalled carbon nanotubes supported nickel oxide nanoparticles composite nanomaterial (NiO@MWNTs), for immobilization of protein and biosensing was designed using a simple and effective hydrothermal method. Using myoglobin (Mb) as a model, the direct electrochemistry of immobilized Mb indicated the matrix could accelerate the electron transfer between protein's active sites and the electrode. The modified electrode shows excellent electrocatalytic activity toward the reduction of H 2 O 2 without the help of an electron mediator. The simple operation, fast response, acceptable stability, and reproducibility of the proposed biosensor indicated its promising application in protein immobilization and preparation of the third generation biosensors.

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Direct electron transfer of horseradish peroxidase and its electrocatalysis based on carbon nanotube/thionine/gold composites

Cited by 63 publications

References 34 publications

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

Laccase Immobilization on Poly(p-Phenylenediamine)/Fe3O4 Nanocomposite for Reactive Blue 19 Dye Removal

Recent applications of carbon-based nanomaterials in analytical chemistry: Critical review

Direct electrochemistry of myoglobin immobilized in NiO/MWNTs hybrid nanocomposite for electrocatalytic detection of hydrogen peroxide

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