Direct electron transfer reactions of laccases from different origins on carbon electrodes

Shleev, Sergey; Jarosz-Wilkołazka, Anna; Khalunina, Anna; Морозова, О. В.; Yaropolov, A. I.; Ruzgas, Tautgirdas; Gorton, Lo

doi:10.1016/j.bioelechem.2005.02.004

Cited by 223 publications

(226 citation statements)

References 47 publications

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“…This value is in agreement with open-circuit potentials of carbon electrodes modified with high redox potential Trametes Lcs, reported to be in the range from 670 up to 910 mV depending on electrode material, Lc source, solution pH and amount of enzyme on the electrode surface [3,7,13,14]. We also found that potentials established were almost independent of O 2 concentrations.…”

Section: Electrochemical Characterisation Of Air Diffusion 'Floating'supporting

confidence: 91%

“…the absence of formation of H 2 O 2 during bioelectrocatalytic four-electron reduction of O 2 , was confirmed using the rotating ringdisk electrode method already in 1979 [7]. Detailed fundamental bioelectrochemical investigations of BMCO were performed using various planar electrodes [14]. It was discovered that carbon was superior, providing efficient O 2 bioelectroreduction at high potentials, whereas DET-based bioelectrocatalysis at metal electrodes seems to be much more complicated [20][21][22].…”

Section: Introductionmentioning

confidence: 88%

“…Taking into account, BMCO inhibition by halide ions, viz. F -and Cl -ions, NaClO 4 was usually used as base electrolyte [13,14,24,28], but other base electrolytes such as KNO 3 and Na 2 SO 4 can also be employed. In the present study, the activity dependence of native and immobilised Lc on solution ionic strength adjusted using KNO 3 was investigated.…”

Section: Investigations Of Native and Immobilised Trametes Hirsuta Lamentioning

confidence: 99%

“…Later this occurrence was identified in many other redox enzymes including different BMCO, e.g. ascorbate oxidase [8,9] and bilirubin oxidase [10,11], as well as fungal [12][13][14][15], plant [12,16] and bacterial [17,18] Lcs. BMCO contain four copper ions which are historically classified into three types according to their spectroscopic characteristics, viz.…”

Section: Introductionmentioning

confidence: 99%

“…Ref. [14]. Briefly, electrodes, on which enzymes are appropriately adsorbed with the T1 site in close proximity to the electrode surface (Figure 1, bottom), can serve as electron donors for the active centre of the enzyme, where molecular O 2 is reduced to H 2 O without formation of highly reactive oxygen species.…”

Section: Introductionmentioning

confidence: 99%

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Stable ‘Floating' Air Diffusion Biocathode Based on Direct Electron Transfer Reactions Between Carbon Particles and High Redox Potential Laccase

et al. 2010

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We report on the assembly and characterisation of a high potential, stable, mediator‐less and cofactor free biocathode based on a fungal laccase (Lc), adsorbed on highly dispersed carbonaceous materials. First, the stability and activity of Trametes hirsuta Lc immobilised on different carbon particles were studied and compared to the solubilised enzyme. Based on the experimental results and a literature analysis, the carbonaceous material BM‐4 was chosen to design efficient and stable biocatalysts for the production of a ‘floating' air diffusion Lc‐based biocathode. Voltammetric characteristics and operational stability of the biocathode were investigated. The current density of oxygen reduction at the motionless biocathode in a quiet, air saturated citrate buffer (100 mM, pH 4.5, 23 °C) reached values as high as 0.3 mA cm–2 already at 0.7 V versus NHE. The operational stability of the biocathode depended on the current density of the device. For example, at low current density (20 μA cm–2), the biocathode lost only 5× of its initial power after 1 month of continuous operation. However, when the device was polarised at 150 mV it lost more than 32× of its initial power in just 10 min. We also found that co‐immobilisation of Lc and peroxidase on highly dispersed carbon materials could protect the biocatalyst from rapid inactivation by hydrogen peroxide produced during electrocatalytic reactions at high‐current densities.

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Section: Electrochemical Characterisation Of Air Diffusion 'Floating'supporting

confidence: 91%

Section: Introductionmentioning

confidence: 88%

Section: Investigations Of Native and Immobilised Trametes Hirsuta Lamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Stable ‘Floating' Air Diffusion Biocathode Based on Direct Electron Transfer Reactions Between Carbon Particles and High Redox Potential Laccase

et al. 2010

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Electrical “Wiring” of Enzymes

Polsky

Brozik

Xiao

et al. 2013

Wiley Encyclopedia of Electrical and Electronics Engineering

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Direct communication with the redox active center of many enzymes can be extremely beneficial for many applications ranging from basic research to the construction of sensors and biofuel cells. Unfortunately, most enzymes have evolved such that they naturally protect their active sites, which are shielded within their protein shell, to work more effectively in native environments. Therefore, much activity has taken place to try and artificially connect external electrode transducers to enzyme redox centers in what is termed electrical “wiring” of enzymes. This review gives a general overview of enzyme wiring including background, methods, and applications.

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Biosensors, Toxicity Monitoring

Ornatska

Ganesana

Andreescu

2009

Encyclopedia of Industrial Biotechnology

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The need for simple, rapid, cost‐effective, and field‐portable screening methods has boosted development in the field of biosensors. This article reviews recent advances in the field of toxicity biosensors for monitoring chemical and biological toxicants, with a focus on how such technologies can be adapted to toxicity monitoring. Current efforts in this direction are concentrated toward disposable devices for screening and semiquantitative analysis. These can be designed to detect a single specific analyte or can provide general or group toxicity screening, depending on the design parameters and the specificity of the biomolecule. Relevant, specific applications and the analytical performance of biosensors for toxicity monitoring of several classes of chemical toxins are discussed.

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Direct electron transfer reactions of laccases from different origins on carbon electrodes

Cited by 223 publications

References 47 publications

Stable ‘Floating' Air Diffusion Biocathode Based on Direct Electron Transfer Reactions Between Carbon Particles and High Redox Potential Laccase

Stable ‘Floating' Air Diffusion Biocathode Based on Direct Electron Transfer Reactions Between Carbon Particles and High Redox Potential Laccase

Electrical “Wiring” of Enzymes

Biosensors, Toxicity Monitoring

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