1999
DOI: 10.1074/jbc.274.16.10802
|View full text |Cite
|
Sign up to set email alerts
|

Direct EPR Detection of the Carbonate Radical Anion Produced from Peroxynitrite and Carbon Dioxide

Abstract: The biological effects of peroxynitrite have been recently considered to be largely dependent on its reaction with carbon dioxide, which is present in high concentrations in intra-and extracellular compartments. Peroxynitrite anion (ONOO ؊ ) reacts rapidly with carbon dioxide, forming an adduct, nitrosoperoxocarboxylate (ONOOCO 2 ؊ ), whose decomposition has been proposed to produce reactive intermediates such as the carbonate radical (CO 3 . ). Here, by the use of rapid mixing continuous flow electron param… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

8
220
1
10

Year Published

1999
1999
2009
2009

Publication Types

Select...
7
2

Relationship

2
7

Authors

Journals

citations
Cited by 295 publications
(239 citation statements)
references
References 35 publications
8
220
1
10
Order By: Relevance
“…Under these circumstances, other nitric oxide synthases have previously been shown to produce • NO and O 2 • − simultaneously, leading to peroxynitrite production [42;46;47], which is a transition metal-independent source of • OH [48][49][50][51][52]. Although peroxynitrite homolysis to • OH is expected to be a minor route in vivo, such a reaction may acquire significant relevance in hydrophobic environments and initiate lipid peroxidation [53].…”
Section: Discussionmentioning
confidence: 99%
“…Under these circumstances, other nitric oxide synthases have previously been shown to produce • NO and O 2 • − simultaneously, leading to peroxynitrite production [42;46;47], which is a transition metal-independent source of • OH [48][49][50][51][52]. Although peroxynitrite homolysis to • OH is expected to be a minor route in vivo, such a reaction may acquire significant relevance in hydrophobic environments and initiate lipid peroxidation [53].…”
Section: Discussionmentioning
confidence: 99%
“…What is a clinician to think of an intervention, which on the one hand has potentially beneficial immune-modulating effects on cytokine networks (52, 55-57), protects lung barrier function (reviewed in Kregenow and Swenson [13]), has complex effects on reactive intermediates (14)(15)(16)58), but also increases the number of necrotic lung cells? In contrast to apoptosis, which may also be enhanced in a hypercapnic environment (18,59), cell death by necrosis triggers a proinflammatory response.…”
Section: Clinical Relevancementioning
confidence: 99%
“…The specific mechanisms through which hypercapnia influences lung injury and vascular barrier function remain uncertain (13). CO 2 generates H ϩ ions, which react with titratable groups in certain amino acids and/or interacts directly with free amine groups in proteins to form carbamate residues (14)(15)(16). CO 2 -dependent effects on the generation of reactive oxygen species and reactive nitrogen species as well as effects on ion channel conductivity have all been considered (17,18).…”
mentioning
confidence: 99%
“…EPR spectra of transient species formed at room temperature (25 Ϯ 2°C) were obtained with an EMX spectrometer equipped with a ER 4117 D-MVT dielectric-mixing resonator (Bruker, Billerica, MA) (68). The magnetic field was calibrated with 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPOL) which has a g-value of 2.0056 (69).…”
Section: Preparation Of Liposomes Containing Phospholipid Hydroperoximentioning
confidence: 99%