Direct estimation of ethanol as a negative peak from alcoholic beverages and fermentation broths by reversed phase-HPLC

Mohanan, Nisha; Shankar, Mukund; Krishnan, Nagasathiya; Saleena, Lilly M.; Rajesh, Mathur; Vairamani, M.

doi:10.1039/c6ay01075j

Cited by 10 publications

(3 citation statements)

References 18 publications

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“…The conditions for the detection of sugars are as follow: injection volume: 20 μL, mobile phase; acetonitrile: water (50:50), flow rate: 1.0 mL/min, the column temperature: 30°C, run time: 7 min, and peaks were recorded at 245 nm [31]. The mobile phase for ethanol detection was 4% acetonitrile in water with 5 min run time; the negative peak was recorded at wavelength of 240 nm using photodiode analyzer (SPDM20A) detector; and the commercial ethanol (99.9%) was used as control [32].…”

Section: Determination Of Sugar and Ethanol Using High-performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Microwave-assisted pretreatment using alkali metal salt in combination with orthophosphoric acid for generation of enhanced sugar and bioethanol

Bhardwaj

Kumar

Verma

2020

Biomass Conv. Bioref.

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The microwave-assisted alkali salt (NaCl, MgCl 2 , and KCl) in combination with H 3 PO 4 pretreatment of rice straw followed by enzymatic hydrolysis with in-house and commercial enzyme cocktail (cellulase and xylanase) was investigated. The effect of pretreatment on the structure and composition of RS was investigated using XRD and FTIR analysis, and the result suggested strategic structural changes in the RS structure. The maximum CrI of 51.3% was obtained with KCl-H 3 PO 4 pretreatment system which is in line with maximum saccharification per biomass of 79% using commercial xylanase and cellulase enzyme cocktail (Com CK -XylCell). Comparable saccharification per biomass of 74% was obtained with in-house xylanase and cellulase enzyme cocktail (Cr CK -XylCell).The simultaneous saccharification and fermentation by KCl-H 3 PO 4 pretreated system of RS resulted the maximum sugar yield of 30.6 and 26.9% and ethanol yield of 12.2 and 11.9 g/L using Com CK -XylCell and Cr CK -XylCell cocktail, respectively, along with Saccharomyces cerevisiae MTCC 173. The hydrolysis of pretreated RS pulp using in-house enzyme cocktail resulted in high sugar and ethanol yield suggesting that the studied alkali metal in combination with weak acid such as H 3 PO 4 can serve as a cost-effective and environment friendly pretreatment method for high production of sugar and ethanol.

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Section: Determination Of Sugar and Ethanol Using High-performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Microwave-assisted pretreatment using alkali metal salt in combination with orthophosphoric acid for generation of enhanced sugar and bioethanol

Bhardwaj

Kumar

Verma

2020

Biomass Conv. Bioref.

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“…Samples were prepared by centrifuging the broth; 50 g of supernatant was distilled (79 ± 1 °C) to collect a uniform weight of 10 g. 10 µL of which was subjected to HPLC. The values were calculated from linear regression of the standards (Nisha et al 2016 ).…”

Section: Methodsmentioning

confidence: 99%

Enhanced saccharification of lignocellulosic agricultural biomass and increased bioethanol titre using acclimated Clostridium thermocellum DSM1313

et al. 2017

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Consolidated bioprocess assures an efficient lignocellulosic conversion to fermentable sugars and subsequently to bioethanol. Such a single-step hydrolysis and anaerobic fermentation was achieved with acclimated Clostridium thermocellum DSM 1313 on different mildly pre-treated agricultural lignocellulosic residues without any additional enzymes/and strains. Acclimation was achieved by serially sub-culturing in increasing concentration of individual substrates, such as rice husk, sugarcane bagasse, and banana pseudostem in the standard media, with cellobiose as an adjunct. The acclimated cellulolytic thermophile exhibited an early log phase entry with enhanced growth compared to the direct inoculation experiments with unacclimated culture. Around 672 mg/g of reducing sugar was produced from sugarcane bagasse media and 636 mg/g from rice husk media and 513 mg/g from banana pseudostem media with the acclimated organism. Bioethanol production also doubled in experiments with serially acclimated cultures, with a maximum of 1.21 and 1.0 g/L ethanol titre from sugarcane bagasse and rice husk, respectively. The serial acclimation experiments have increased the saccharification potentials of the organism towards the respective lignocellulosic substrates and also enhanced the bioethanol production.

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“…Ethanol absorbs less UV compared to high UV absorbing background, which result in the reverse positive or negative peak polarity. Direct estimation of ethanol as a negative peak from samples was con rmed by reversed phase-HPLC and performed same according to (Nisha et al 2016). In every run, the three peaks were corresponding to acetone, ethanol or water singly or in combination were detected.…”

Section: Ethanol Estimationmentioning

confidence: 99%

Simultaneous Production of Bioethanol and Bioelectricity in a Membrane-Less Single-Chambered Yeast Fuel Cell by Saccharomyces cerevisiae and Pichia fermentans

2021

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Production of bioethanol and bioelectricity is a promising approach through microbial electrochemical technology. Sugars are metabolized by yeast to produces ethanol, CO 2 and energy. Surplus electrons produced during the fermentation can be transferred through the circuit to generate electricity in a Microbial fuel cell (MFC). In the present study, a membrane less single chambered microbial fuel cell was developed for simultaneous production of bioethanol and bioelectricity. Pichia fermentans along with a well-known ethanol producing yeast Saccharomyces cerevisiae was allowed to ferment glucose. S. cerevisiae demonstrated maximum open circuit voltage (OCV) 0.287 ± 0.009 V and power density 4.473 mW m − 2 on 15th day, with a maximum ethanol yield of 5.6% (v/v) on 12th day. P. fermentans demonstrated a maximum OCV of 0.318 ± 0.0039 V and power density of 8.299 mW m − 2 on 15th day with ethanol yield of 4.7 % (v/v) on 12th day. Coulombic e ciency (CE) increased gradually from 0.002-0.471 % and 0.012-0.089 % in the case of S. cerevisiae and P. fermentans, respectively, during 15 days of experiment. Thus, the result indicated that Single chambered fuel cell can be explored for its potential applications for ethanol production along with clean energy generation.

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Direct estimation of ethanol as a negative peak from alcoholic beverages and fermentation broths by reversed phase-HPLC

Abstract: Direct injection of alcoholic samples/broths into a RP-HPLC (with a PDA-UV detector) system to quantify ethanol directly as a negative peak. The kind of peak pair depends on the ethanol concentration as described in the article.

Cited by 10 publications

References 18 publications

Microwave-assisted pretreatment using alkali metal salt in combination with orthophosphoric acid for generation of enhanced sugar and bioethanol

Microwave-assisted pretreatment using alkali metal salt in combination with orthophosphoric acid for generation of enhanced sugar and bioethanol

Enhanced saccharification of lignocellulosic agricultural biomass and increased bioethanol titre using acclimated Clostridium thermocellum DSM1313

Simultaneous Production of Bioethanol and Bioelectricity in a Membrane-Less Single-Chambered Yeast Fuel Cell by Saccharomyces cerevisiae and Pichia fermentans

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