Direct ethanol production from cassava pulp using a surface-engineered yeast strain co-displaying two amylases, two cellulases, and β-glucosidase

Apiwatanapiwat, Waraporn; Murata, Yoshinori; Kosugi, Akihiko; Yamada, R.; Kondo, Akihiko; Arai, T.; Rugthaworn, Prapassorn; Mori, Yutaka

doi:10.1007/s00253-011-3115-8

Cited by 55 publications

(24 citation statements)

References 29 publications

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“…The reaction was then performed at 45 °C for 10 min and stopped by adding 150 µL of 1 mol L ‐1 Na 2 CO 3 . After 2 min centrifugation, the amount of p ‐nitrophenol released was measured at 400 nm …”

Section: Methodsmentioning

confidence: 99%

The combined use of a continuous‐flow microchannel reactor and ionic liquid cosolvent for efficient biocatalysis of unpurified recombinant enzyme

Wang

Zhu

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Microfluidic biocatalysis has advantages in terms of heat transfer and mass transfer. However, mass transfer in microscale conditions is often limited by excessive enzyme agglomeration, resulting in reduced reactor performance. In the present study, to establish an efficient and inexpensive microreaction system for obtaining high‐value fine chemicals, a novel combined use of continuous‐flow microchannel reactor and ionic liquid cosolvent for efficient production of isoquercitrin from rutin catalysed by unpurified recombinant enzyme RhaB1 was successfully carried out. RESULTS The unpurified RhaB1 was expressed by BL21‐pET21a‐rhaB1 and the amino acid sequence was confirmed by Maldi‐TOF‐TOF, which was directly applied in microchannel reactors with an increased activity of 37.7% compared with the purified RhaB1, with [Toma][Tf2N] used as a cosolvent to avoid enzymatic agglomeration. A yield of 99.3 ± 4.3% was achieved in 10 min using crude RhaB1 in microchannel reactors under optimum conditions, and circular dichroism spectroscopy confirmed that [Toma][Tf2N] had a positive effect on RhaB1 activity. Compared with a batch reactor, the reaction time was reduced by 98.3%, the Km was decreased to 1/3 and productivity was improved 61.4 times. CONCLUSION Results indicated that the developed cosolvent system in the microfluidic reactor could improve the solubility and liquidity of recombinant RhaB1. The protocol is promising for industrial applications due to higher productivity and lower costs. © 2018 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

The combined use of a continuous‐flow microchannel reactor and ionic liquid cosolvent for efficient biocatalysis of unpurified recombinant enzyme

Wang

Zhu

et al. 2018

J of Chemical Tech & Biotech

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“…In this respect, heterologous β-glucosidases genes with different origin have been successfully cloned and expressed in S. cerevisiae strains, including those from Saccharomycopsis fibuligera [146,147], Issatchenkia orientalis [148]. Furthermore, to reduce the cellulosic ethanol production cost, a promising strategy termed as "consolidated bioprocessing" has been proposed, which involves production of all the cellulolytic enzymes (EG, CBH, BG) and fermentation of resulting sugars to ethanol [149][150][151]. Considerable success has been achieved enabling growth of recombinant S. cerevisiae on pure cellulose [152,153].…”

Section: Strain Improvementmentioning

confidence: 99%

Bioconversion of Lignocellulose into Bioethanol: Process Intensification and Mechanism Research

Huang

et al. 2011

Bioenerg. Res.

125

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Biofuels produced from lignocellulosic biomass can significantly reduce the energy dependency on fossil fuels and the resulting effects on environment. In this respect, cellulosic ethanol as an alternative fuel has the potential to become a viable energy source in the near future. Over the past few decades, tremendous effort has been undertaken to make cellulosic ethanol cost competitive with conventional fossil fuels. The pretreatment step is always necessary to deconstruct the recalcitrant structures and to make cellulose more accessible to enzymes. A large number of pretreatment technologies involving physical, chemical, biological, and combined approaches have been developed and tested at the pilot scale. Furthermore, various strategies and methods, including multi-enzyme complex, non-catalytic additives, enzyme recycling, high solids operation, design of novel bioreactors, and strain improvement have also been implemented to improve the efficiency of subsequent enzymatic hydrolysis and fermentation. These technologies provide significant opportunities for lower total cost, thus making large-scale production of cellulosic ethanol possible. Meanwhile, many researchers have focused on the key factors that limit cellulose hydrolysis, and analyzing the reaction mechanisms of cellulase. This review describes the most recent advances on process intensification and mechanism research of pretreatment, enzymatic hydrolysis, and fermentation during the production of cellulosic ethanol.

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“…Aspergillus aculeatus β-glucosidase I has been reported that it can hydrolyze soluble and insoluble cello-oligosaccharides. Apiwatanapiwat et al [8] modified a yeast strain coexpressing α-amylase, glucoamylase, endoglucanase, cellobiohydrase, and β-glucosidase that could directly produce ethanol from cassava pulp without addition of any hydrolytic enzymes during fermentation. Ito et al [9] constructed a yeast strain for APG synthesis from genetically inducing the display of β-glucosidase I from A. aculeatus No.…”

Section: Introductionmentioning

confidence: 99%

Production of Alkyl Polyglucoside Using Pichia pastoris GS115 Displaying Aspergillus aculeatus β-Glucosidase I

Kang

Wei

Guo

et al. 2015

Lecture Notes in Electrical Engineering

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Pichia pastoris GS115 displaying Aspergillus aculeatus β-glucosidase I was used as whole-cell biocatalyst catalyzing the synthesis of 1-hexyl glucoside, 1-octyl glucoside, and 1-decyl glucoside through reverse hydrolysis in water-alcohol two-phase system, while glucose and primary alcohols were used as substrate. In this study, factors affecting the synthetic yield were optimized in our study, including water content, glucose concentration, whole-cell biocatalyst content, the pH value of sodium acetate buffer, and the reaction temperature. In 5 mL reaction volume, the optimal reaction conditions of HG were: 10 % pH 3.0 buffer, 0.2 mol/L glucose, 10 g/L whole-cell biocatalyst; the optimal reaction conditions of DG: 15 % pH 3.0 buffer, 0.6 mol/L glucose, 10 g/L whole-cell biocatalyst; the optimal reaction conditions of DG: 20 % pH 2.0 buffer, 0.2 mol/L glucose, 40 g/L whole-cell biocatalyst; three reactions were conducted under 55°C. After 72 h the maximum yield of HG was 11.5 %, that of OG and DG were 5.3 and 3.2 % after 96 h, respectively. The reutilization experiments inducted that the whole-cell biocatalyst could be used at least for five times without obvious decrease in synthesis ability.

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Direct ethanol production from cassava pulp using a surface-engineered yeast strain co-displaying two amylases, two cellulases, and β-glucosidase

Cited by 55 publications

References 29 publications

The combined use of a continuous‐flow microchannel reactor and ionic liquid cosolvent for efficient biocatalysis of unpurified recombinant enzyme

The combined use of a continuous‐flow microchannel reactor and ionic liquid cosolvent for efficient biocatalysis of unpurified recombinant enzyme

Bioconversion of Lignocellulose into Bioethanol: Process Intensification and Mechanism Research

Production of Alkyl Polyglucoside Using Pichia pastoris GS115 Displaying Aspergillus aculeatus β-Glucosidase I

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