Direct ETTIN-auxin interaction controls chromatin states in gynoecium development

Kuhn, André; Harborough, Sigurd Ramans; McLaughlin, Heather; Natarajan, Bhavani; Verstraeten, Inge; Friml, Jiřı́; Kepinski, Stefan; Østergaard, Lars

doi:10.7554/elife.51787

Cited by 54 publications

(65 citation statements)

References 50 publications

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“…For example, the atypical ARF ETTIN (ETT) cannot recruit AUX/ IAA adaptors and instead directly recruits TPLs, via a BRD repression domain, to repress auxin target genes. Remarkably, ETT also acts as an auxin receptor, and auxin binding by ETT disrupts the ETT-TPL interaction, leading to de-repression of ETT targets (Kuhn et al, 2020).…”

Section: Hormone Signallingmentioning

confidence: 99%

“…All genetic components of the canonical [ARF]‐[AUX/IAA]‐[TPL/TPR] mechanism regulating auxin responses are conserved from Charophyte algae through to the angiosperms (Flores‐Sandoval et al, 2015; Martin‐Arevalillo et al, 2019), although it remains to be determined whether the mechanism is functionally conserved. However, additional non‐canonical auxin signalling mechanisms that utilise TPL corepressors have been identified (Causier et al, 2012a,b; Kuhn et al, 2020). For example, the atypical ARF ETTIN (ETT) cannot recruit AUX/IAA adaptors and instead directly recruits TPLs, via a BRD repression domain, to repress auxin target genes.…”

Section: Tpl/tprs Are Recruited Into Diverse Biological Processesmentioning

confidence: 99%

“…Interestingly, TPL proteins have been shown to interact directly with certain ARF proteins in Arabidopsis , P. patens and the charophyte alga Chlorokybus atmophyticus , even in the absence of AUX/IAA adaptors (Causier et al, 2012a,b; Martin‐Arevalillo et al, 2019). As discussed above, the interaction between TPL and the atypical Arabidopsis ARF ETT is disrupted by auxin binding to ETT (Kuhn et al, 2020). Together these findings suggest the existence of a precursor classical auxin response pathway that predates the canonical auxin response.…”

Section: Topless Predates the Evolution Of Land Plantsmentioning

confidence: 99%

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Repressor for hire! The vital roles of TOPLESS‐mediated transcriptional repression in plants

2021

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Transcriptional corepressors play important roles in establishing the appropriate levels of gene expression during growth and development. The TOPLESS (TPL) family of corepressors are critical for all plant life. TPLs are involved in numerous developmental processes and in the response to extrinsic challenges. As such these proteins have been the focus of intense study since Long and colleagues first described the TPL corepressor in 2006. In this review we will explore the evolutionary history of these essential plant-specific proteins, their mechanism of action based on recent structural analyses, and the myriad of pathways in which they function. We speculate how relatively minor changes in the peptide sequence of transcriptional regulators allowed them to recruit TPL into new processes, driving innovation and resulting in TPL becoming vital for plant development.

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Section: Hormone Signallingmentioning

confidence: 99%

Section: Tpl/tprs Are Recruited Into Diverse Biological Processesmentioning

confidence: 99%

Section: Topless Predates the Evolution Of Land Plantsmentioning

confidence: 99%

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Repressor for hire! The vital roles of TOPLESS‐mediated transcriptional repression in plants

2021

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“…This in turn, releases repression of Auxin Response Element-(ARE)-containing promoters, simply put, as a consequence of derepressed AUXIN RESPONSE FACTOR (ARF) transcription factors no longer dimerizing with Aux/IAAs [ 36 ]. Alternate auxin sensing mechanisms have been identified, which involves activity of plasma membrane-resident TRANSMEMBRANE KINASE (TMK) proteins [ 37 , 38 , 39 ] as well as hormone sensing via the ARF3/ETTIN transcriptional regulator [ 40 , 41 ]. Modes of auxin perception via these pathways are still only partially resolved and matter of discussion [ 42 , 43 ], but downstream signaling events have been characterized to some extent.…”

Section: Auxinmentioning

confidence: 99%

Auxin and Root Gravitropism: Addressing Basic Cellular Processes by Exploiting a Defined Growth Response

Konstantinova¹,

Korbei²,

Luschnig³

2021

IJMS

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Root architecture and growth are decisive for crop performance and yield, and thus a highly topical research field in plant sciences. The root system of the model plant Arabidopsis thaliana is the ideal system to obtain insights into fundamental key parameters and molecular players involved in underlying regulatory circuits of root growth, particularly in responses to environmental stimuli. Root gravitropism, directional growth along the gravity, in particular represents a highly sensitive readout, suitable to study adjustments in polar auxin transport and to identify molecular determinants involved. This review strives to summarize and give an overview into the function of PIN-FORMED auxin transport proteins, emphasizing on their sorting and polarity control. As there already is an abundance of information, the focus lies in integrating this wealth of information on mechanisms and pathways. This overview of a highly dynamic and complex field highlights recent developments in understanding the role of auxin in higher plants. Specifically, it exemplifies, how analysis of a single, defined growth response contributes to our understanding of basic cellular processes in general.

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“…Accordingly, TPL, TPR2 and TPR4 acting together seem to regulate the DCL1 and SERRATE expression, while TPR4 alone may be involved in the repression of HEN1. The gene silencing complexes commonly consist of different TPL/TPR proteins [65,66]. However, recently, a monomeric form of TPL was found to be sufficient to cause an intense transcriptional repression in plants [67].…”

Section: Agl15 Together With the Tpl/tpr Corepressors Transcriptionalmentioning

confidence: 99%

AGL15 Controls the Embryogenic Reprogramming of Somatic Cells in Arabidopsis through the Histone Acetylation-Mediated Repression of the miRNA Biogenesis Genes

Nowak¹,

Morończyk²,

Wójcik³

et al. 2020

IJMS

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The embryogenic transition of somatic cells requires an extensive reprogramming of the cell transcriptome. Relevantly, the extensive modulation of the genes that have a regulatory function, in particular the genes encoding the transcription factors (TFs) and miRNAs, have been indicated as controlling somatic embryogenesis (SE) that is induced in vitro in the somatic cells of plants. Identifying the regulatory relationships between the TFs and miRNAs during SE induction is of central importance for understanding the complex regulatory interplay that fine-tunes a cell transcriptome during the embryogenic transition. Hence, here, we analysed the regulatory relationships between AGL15 (AGAMOUS-LIKE 15) TF and miR156 in an embryogenic culture of Arabidopsis. Both AGL15 and miR156 control SE induction and AGL15 has been reported to target the MIR156 genes in planta. The results showed that AGL15 contributes to the regulation of miR156 in an embryogenic culture at two levels that involve the activation of the MIR156 transcription and the containment of the abundance of mature miR156 by repressing the miRNA biogenesis genes DCL1 (DICER-LIKE1), SERRATE and HEN1 (HUA-ENHANCER1). To repress the miRNA biogenesis genes AGL15 seems to co-operate with the TOPLESS co-repressors (TPL and TPR1-4), which are components of the SIN3/HDAC silencing complex. The impact of TSA (trichostatin A), an inhibitor of the HDAC histone deacetylases, on the expression of the miRNA biogenesis genes together with the ChIP results implies that histone deacetylation is involved in the AGL15-mediated repression of miRNA processing. The results indicate that HDAC6 and HDAC19 histone deacetylases might co-operate with AGL15 in silencing the complex that controls the abundance of miR156 during embryogenic induction. This study provides new evidence about the histone acetylation-mediated control of the miRNA pathways during the embryogenic reprogramming of plant somatic cells and the essential role of AGL15 in this regulatory mechanism.

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Direct ETTIN-auxin interaction controls chromatin states in gynoecium development

Cited by 54 publications

References 50 publications

Repressor for hire! The vital roles of TOPLESS‐mediated transcriptional repression in plants

Repressor for hire! The vital roles of TOPLESS‐mediated transcriptional repression in plants

Auxin and Root Gravitropism: Addressing Basic Cellular Processes by Exploiting a Defined Growth Response

AGL15 Controls the Embryogenic Reprogramming of Somatic Cells in Arabidopsis through the Histone Acetylation-Mediated Repression of the miRNA Biogenesis Genes

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