Direct Evidence for a Colchicine-Induced Impairment in the Mobility of Membrane Components

Wunderlich, Frank; Müller, Rebecca; Speth, Volker

doi:10.1126/science.182.4117.1136

Cited by 142 publications

(32 citation statements)

References 41 publications

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“…Consideration should also be given to a possible direct effect of colchicine on membranes and their topography . Colchicine impaired the movement of membrane particles in the alveolar membrane of Tetrahymena pyriformis (49) and bound to rat liver Golgi membranes and increased their fluidity (23) . Such effects might explain the influence of colchicine in inhibiting the selective uptake of concanavalin A binding sites during phagocytosis by polymorphonuclear leukocytes (27), concanavalin A-induced agglutination of hepatoma cells (24), and the appearance of augmented amino acid transport capacity after partial hepatectomy (46), although an influence via microtubular effects cannot be excluded.…”

Section: Discussionmentioning

confidence: 99%

Effect of colchicine on the uptake of prolactin and insulin into Golgi fractions of rat liver.

Posner¹,

Verma²,

Patel³

et al. 1982

The Journal of Cell Biology

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In previous studies we have shown that 125I-labeled prolactin is taken up by a receptor-dependent process and concentrated in an intact form in Golgi elements from female rat liver (J. Biol. Chem., 1979, 254:209-214). In this study we have examined the effect of colchicine on this uptake process into Golgi elements. Colchicine [25 mumol (10 mg)/100 gm body wt] was injected intraperitoneally in adult female rats, and hepatic Golgi fractions were prepared at 1, 2, and 3 h postinjection. The enzyme recoveries and morphological appearance of fractions from colchicine-treated and control (alcohol alone) animals were similar. At times greater than 1 h after colchicine there was a marked (greater than 60%) inhibition of uptake of 125I-ovine prolactin (125I-oPRL) into Golgi light and intermediate fractions but no inhibition of uptake into Golgi heavy and plasmalemma elements. At times from 2 to 45 min postinjection, 125I-oPRL was extracted from Golgi elements and found to be largely intact as judged by rebinding to receptors. The inhibitory effect of colchicine was seen at doses ranging from 0.25 mumol to 25 mumol/100 g body wt. Vincristine also inhibited 125I-oPRL uptake into the Golgi light and intermediate fractions but lumicolchicine had no inhibitory effect. There was a smaller effect of colchicine both at early (1 h) and later (3 h) times on the extent and pattern of 125I-insulin uptake. Colchicine treatment did not produce a significant change in lactogen receptor levels in the Golgi fractions. These results demonstrate that colchicine treatment inhibited the transfer of prolactin into Golgi vesicular elements. The much smaller effect on insulin uptake suggests that there may be differences in the manner in which the two hormones are handled in the course of internalization.

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Section: Discussionmentioning

confidence: 99%

Effect of colchicine on the uptake of prolactin and insulin into Golgi fractions of rat liver.

Posner¹,

Verma²,

Patel³

et al. 1982

The Journal of Cell Biology

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“…During dedifferentiation and loss of specific cell functions such as protein secretion (32), the elaborate transport systems may also dedifferentiate, and uptake of nutrients would possibly be achieved by simple processes such as micropinocytosis, for example. These new uptake mechanisms could depend on variables such as plasma membrane microviscosity or the lateral mobility of membrane components, both of which are affected by colchicine (10,11). The effect of colchicine and vinblastine could be due to changes in composition (33) and turnover (15,34) of membrane proteins and glycoproteins in hepatomas.…”

Section: Methodsmentioning

confidence: 99%

“…Microtubule-associated functions may also regulate lysosomal degranulation during phagocytosis (7,8) and influence the conversion of prohormones into their active forms (9). The colchicine-induced inhibition of the mobility of membrane components (10) and changes in plasma membrane microviscosity associated with phagocytosis (11) may reflect the close relationship between the microtubular system and the cell membrane.…”

mentioning

confidence: 99%

A colchicine-sensitive uptake system in Morris hepatomas.

Tauber¹,

Reutter²

1980

Proc. Natl. Acad. Sci. U.S.A.

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' The interference of microtubular disruptors with the-uptake of amino acids and other low molecular weight substrates has been studied in Morris hepatomas, host liver, and regenerating liver. Colchicine inhibits amino acid transport (ct-aminoisobutyric acid, L-methionine, and L-leucine) in hepatomas by 59-98% whereas transport in host and regenerating liver is not impeded but increased. In hepatomas, treatment with colchicine also reduces the uptake of L-fcose, cytidine, urea, and carbonate. Vinblastine, but not lumicolchicine or cytochalasin B, is an effective inhibitor. The inhibition of uptake is not linked to a decrease of cellular ATP and UTP. The data suggest that the transport of low molecular weight substrates in hepatomas is related to microtubules or other colchicine-binding structures, e.g., of the plasma membrane. This colchicine-sensitive uptake system in hepatomas may be due to the malignant transformation of hepatocytes.

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“…Examination of the reports of some authors will lead us to a mechanism which concerns both binding to membranes and microtubules. Wunderlich et al (1973), Furcht and Scott (1975) and Furcht et al (1976) showed that colchicine alters mobility or topography of membrane components on normal or transformed cells. On the other hand, Tamura (1971) showed blockade of transference of newly synthesized microtubule protein to particulate fraction within colchicine treated cells.…”

mentioning

confidence: 99%

Cytoskeleton, microtubules, tubulin and colchicine : A review.

Artvinli

1987

CYTOLOGIA

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Direct Evidence for a Colchicine-Induced Impairment in the Mobility of Membrane Components

Cited by 142 publications

References 41 publications

Effect of colchicine on the uptake of prolactin and insulin into Golgi fractions of rat liver.

Effect of colchicine on the uptake of prolactin and insulin into Golgi fractions of rat liver.

A colchicine-sensitive uptake system in Morris hepatomas.

Cytoskeleton, microtubules, tubulin and colchicine : A review.

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