Direct Evidence for Requirement of Phosphatidylglycerol in Photosystem II of Photosynthesis

Hagio, Miki; Gombos, Zoltán; Várkonyi, Zsuzsanna; Masamoto, Kazumori; Sato, Norihiro; Tsuzuki, Mikio; Wada, Hajime

doi:10.1104/pp.124.2.795

Cited by 183 publications

(101 citation statements)

References 34 publications

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“…The A 1A redox potential is by 155 mV more positive than that of A 1B , which is because of electrostatic interactions with the backbone and the conserved residue Asp-B575. The influence of the asymmetrically distributed lipids on the A 1 redox potentials turned out to be small, in agreement with a recent mutant study (71), despite the negative net charge on PG.…”

Section: Resultssupporting

confidence: 90%

Redox Potential of Quinones in Both Electron Transfer Branches of Photosystem I

Ishikita

Knapp

2003

Journal of Biological Chemistry

108

151

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Section: Resultssupporting

confidence: 90%

Redox Potential of Quinones in Both Electron Transfer Branches of Photosystem I

Ishikita

Knapp

2003

Journal of Biological Chemistry

108

151

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“…PG is thought to play an important role in the ordered assembly and structural maintenance of the photosynthetic apparatus in thylakoid membranes [1][2][3][4]. It is required in photosystem II of photosynthesis [5,6]. The chloroplast membranes of all photosynthetic eukaryotes contain a high proportion of the unusual fatty 3-trans-hexadecenoic acid [⌬ 3 16:1 or C16:1(3t)], which is always found esterified to the sn-2 of glycerol backbone of PG [7][8][9][10].…”

Section: P Hosphatidylglycerol (Pg) Is the Major Phospholipid Of Planmentioning

confidence: 99%

Electrospray ionization multiple stage quadrupole ion-trap and tandem quadrupole mass spectrometric studies on phosphatidylglycerol from arabidopsis leaves

Hsu

Turk

Williams

et al. 2007

J. Am. Soc. Mass Spectrom.

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Phosphatidylglycerol (PG) is the major phospholipid of plant chloroplasts. PG from Arabidopsis thaliana has an unusual fatty acyl chain, 3-trans-hexadecenoyl (⌬ 3 16:1) in the sn-2 position of the major 18:3/⌬ 3 16:1-PG species, as well as in 18:2/⌬ 3 16:1-PG and 16:0/⌬ 3 16:1-PG. Upon low-energy collisionally activated dissociation (CAD) in a tandem quadrupole or in an ion-trap mass spectrometer, the [M -H] Ϫ ions of the PG molecules containing ⌬ 3 16:1 give product-ion spectra that are readily distinguishable from those arising from PGs without the ⌬ 3 16:1 species. The ⌬ 3 16:1-fatty acyl-containing PGs are characterized by MS 2 product-ion mass spectra that contain predominant [M -H -236] Ϫ ions arising from loss of the ⌬ 3 16:1-fatty acyl substituent as a ketene. This is attributable to the fact that the ␣-hydrogen of the ⌬ 3 16:1-fatty acid substituent involved in the ketene loss is an allylic hydrogen, which is very labile. This leads to preferential neutral loss of 236 and drastic decline in the neutral loss of 254 (i.e., loss as a fatty acid), the unique features that signify the presence of ⌬ 3 16:1-fatty acyl containing PGs. The neutral loss scan of 236, thus, provides a sensitive tandem quadrupole mass spectrometric means to identify ⌬ 3 16:1-containing PG species in lipid mixtures. This low-energy tandem mass spectrometric approach also permits the structures of the Arabidopsis PGs that consist of two isomeric structures to be unveiled. (J Am Soc Mass Spectrom 2007, 18, 783-790)

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“…It was shown earlier that complex lipids like phosphatidylglycerol (PG) can be taken up efficiently by cyanobacteria (Hagio et al, 2000). For the experiment the lipids sulfoquinovosyldiacylglycerol (SQDG) and PG-containing 14 C-labeled palmitic acid and 14 C-labeled oleic acid were generated as described in "Material and Methods."…”

mentioning

confidence: 99%

Fatty Acid Activation in Cyanobacteria Mediated by Acyl-Acyl Carrier Protein Synthetase Enables Fatty Acid Recycling

2010

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In cyanobacteria fatty acids destined for lipid synthesis can be synthesized de novo, but also exogenous free fatty acids from the culture medium can be directly incorporated into lipids. Activation of exogenous fatty acids is likely required prior to their utilization. To identify the enzymatic activity responsible for activation we cloned candidate genes from Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942 and identified the encoded proteins as acyl-acyl carrier protein synthetases (Aas). The enzymes catalyze the ATP-dependent esterification of fatty acids to the thiol of acyl carrier protein. The two protein sequences are only distantly related to known prokaryotic Aas proteins but they display strong similarity to sequences that can be found in almost all organisms that perform oxygenic photosynthesis. To investigate the biological role of Aas activity in cyanobacteria, aas knockout mutants were generated in the background of Synechocystis sp. PCC 6803 and S. elongatus PCC 7942. The mutant strains showed two phenotypes characterized by the inability to utilize exogenous fatty acids and by the secretion of endogenous fatty acids into the culture medium. The analyses of extracellular and intracellular fatty acid profiles of aas mutant strains as well as labeling experiments indicated that the detected free fatty acids are released from membrane lipids. The data suggest a considerable turnover of lipid molecules and a role for Aas activity in recycling the released fatty acids. In this model, lipid degradation represents a third supply of fatty acids for lipid synthesis in cyanobacteria.

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Direct Evidence for Requirement of Phosphatidylglycerol in Photosystem II of Photosynthesis

Cited by 183 publications

References 34 publications

Redox Potential of Quinones in Both Electron Transfer Branches of Photosystem I

Redox Potential of Quinones in Both Electron Transfer Branches of Photosystem I

Electrospray ionization multiple stage quadrupole ion-trap and tandem quadrupole mass spectrometric studies on phosphatidylglycerol from arabidopsis leaves

Fatty Acid Activation in Cyanobacteria Mediated by Acyl-Acyl Carrier Protein Synthetase Enables Fatty Acid Recycling

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