Direct evidence for the nature of the binding of mitochondria to microtubules in ovarian nutritive tubes of an hemipteran insect

Stebbings, Howard

doi:10.1007/s004410050880

Cited by 3 publications

(3 citation statements)

References 30 publications

(35 reference statements)

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“…Despite the similarities, we did not observe the transference of mitochondria from nurse cells to oocytes, as described in some insects (e.g. Tourmente et al, 1990;Stebbings, 1997).…”

Section: Discussionmentioning

confidence: 64%

Ultrastructural studies of oogenesis in <i>Bolinus brandaris</i> (Gastropoda: Muricidae)

Amor¹,

Ramón²,

Durfort³

2004

Sci. Mar.

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SUMMARY: Ultrastructural studies of oogenesis in Bolinus brandaris are described. Although the initial phase of oogenesis is common to most animal species, vitellogenesis can be considered a species-specific characteristic. In the vitellogenesis of B. brandaris, mitochondria and endoplasmic reticula play a relevant role in the formation of myelinised membranous systems. Nuclear envelope, Golgi body and the oocyte plasma membrane invaginations are three possible origins for annulate lamellae. The latter can be considered membranous reservoirs. There are two sources for the vitellum: exogenous (from follicular cells) and endogenous (from the endoplasmic reticulum of the same oocyte).Key words: vitellogenesis, mitochondria, endoplasmic reticulum, annulate lamellae, imposex.RESUMEN: ESTUDIO ULTRAESTRUCTURAL DE LA OOGENESIS DE BOLINUS BRANDARIS (GASTROPODA: MURICIDAE). -Aunque las primeras fases de la oogénesis son comunes en la mayoría de las especies animales, la vitelogénesis sin embargo, puede considerarse específica para cada una de ellas. En la vitelogénesis de Bolinus brandaris, cabe destacar el papel de las mitocondrias y del retículo endoplasmático en la formación de sistemas membranosos que asemejan figuras mielínicas. La envoltura nuclear, el complejo de Golgi y las invaginaciones de la membrana plasmática oocitaria podrían constituir los posibles orígenes de las láminas anilladas, que pueden considerarse un reservorio membranoso. El vitelo a su vez, puede tener dos orígenes: extrínseco (procedente de las células foliculares) e intrínseco (sintetizado por el retículo endoplasmático del propio oocito).Palabras clave: vitelogénesis, mitocondria, retículo endoplasmático, láminas anilladas, imposex.

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“…Despite the similarities, we did not observe the transference of mitochondria from nurse cells to oocytes, as described in some insects (e.g. Tourmente et al, 1990;Stebbings, 1997).…”

Section: Discussionmentioning

confidence: 64%

Ultrastructural studies of oogenesis in <i>Bolinus brandaris</i> (Gastropoda: Muricidae)

Amor¹,

Ramón²,

Durfort³

2004

Sci. Mar.

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“…It is well known that the association of membrane-bound organelles with microtubules is crucial for determining of their shape, intracellular localization and translocation. The importance of microtubular interactions has been demonstrated for numerous organelles, including mitochondria (Stebbings, 1997). In trichomonads, the axostyle seems to participate in the hydrogenosome segregation between the daughter cells.…”

Section: Hydrogenosomes and Microtubulesmentioning

confidence: 99%

Hydrogenosome behavior during the cell cycle in Tritrichomonas foetus

Benchimol

Engelke

2003

Biology of the Cell

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The hydrogenosome is an unusual organelle found in several trichomonad species and other protists living in oxygen poor or anoxic environments. The hydrogenosome behavior in the protist Tritrichomonas foetus, parasite of the urogenital tract of cattle, is reported here. The hydrogenosomes were followed by light and transmission electron microscopy during the whole cell cycle. Videomicroscopy, immunofluorescence microscopy, and immunocytochemistry were also used. It is shown that the hydrogenosomes divide at any phase of the cell cycle and that the organellar division is not synchronized. During the interphase the hydrogenosomes are distributed mainly along the axostyle and costa, and at the beginning of mitosis migrate to around the nucleus. Three forms of hydrogenosome division were seen: (1) segmentation, where elongated hydrogenosomes are further separated by external membranous profiles; (2) partition, where rounded hydrogenosomes, in a bulky form, are further separated by a membranous internal septum and, (3) a new dividing form: heart-shaped hydrogenosomes, which gradually present a membrane invagination leading to the organelle division. The hydrogenosomes divide at any phase of the cell cycle. A necklace of intramembranous particles delimiting the outer hydrogenosomal membrane in the region of organelle division was observed by freeze-etching. Similarities between hydrogenosomes and mitochondria behavior during the cell cycle are discussed.

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“…The generation and maintenance of this organization is particularly dependent on the microtubular system. The importance of microtubular interactions has been demonstrated for numerous organelles, such as secretory granules [1,2], exocytotic [3] and endocytic vesicles [4,5], melanosomes [6], phagosomes [7,8], autophagosomes [9], lysosomes [10,11], endoplasmic reticulum [12,13], Golgi complex [14–17], and mitochondria [18,19]. For peroxisomes, although the interaction with microtubules has been demonstrated [20–24], little is known about the nature of involved proteins.…”

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confidence: 99%

Interaction of peroxisomes with microtubules

Thiemann

Schrader

Völkl

et al. 2000

European Journal of Biochemistry

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The association of membrane-bounded cell organelles to microtubules is crucial for determination of their shape, intracellular localization and translocation. We have shown previously the high affinity binding of peroxisomes to microtubules which appears to be of static nature as in vivo studies indicate that only a few peroxisomes move along the microtubular tracks. In order to characterize the interactions of peroxisomes with microtubules, we have developed a semiquantitative in vitro binding assay, which is based on the association of highly purified rat liver peroxisomes to microtubules coated onto microtiterplates. The binding was visualized by differential interference contrast and immunofluorescence using a confocal laser scanning microscope. The binding was concentration dependent and saturable, being affected by time, temperature, and pH. Addition of ATP or the motor proteins kinesin and dynein increased the binding capacity, while ATP-depletion or microtubule associated proteins (MAPs) decreased it. KCl treatment of peroxisomes reduced the binding, which was restored by dialyzed KCl-stripping eluate as well as by rat liver cytosol. The reconstituting effect of cytosol was abolished by its pretreatment with proteases or N-ethylmaleimide. Moreover, the treatment of peroxisomes with proteases or N-ethylmaleimide reduced their binding, which was not reversed by cytosol. These results suggest the involvement of a peroxisomal membrane protein and cytosolic factor(s) in the binding of peroxisomes to microtubules. This notion is supported by the observation that distinct subfractions of dialyzed KCl-stripping eluate obtained by gel chromatography augmented the binding. Those subfractions, as well as purified peroxisome fractions, exhibited strong immunoreactivity with an antibody to cytoplasmic linker protein (CLIP)-115, revealing a 70-kDa polypeptide. Moreover, immunodepletion of KCl-stripping eluate and its subfractions with an antibody to the conserved microtubule binding domain of CLIPs, abolished their promoting effect on the binding, thus suggesting the involvement of a CLIP-related protein in the binding of peroxisomes to microtubules.

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Direct evidence for the nature of the binding of mitochondria to microtubules in ovarian nutritive tubes of an hemipteran insect

Cited by 3 publications

References 30 publications

Ultrastructural studies of oogenesis in <i>Bolinus brandaris</i> (Gastropoda: Muricidae)

Ultrastructural studies of oogenesis in <i>Bolinus brandaris</i> (Gastropoda: Muricidae)

Hydrogenosome behavior during the cell cycle in Tritrichomonas foetus

Interaction of peroxisomes with microtubules

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