Direct examination of the injection process in liquid chromatographic separations

Evans, Christine; McGuffin, Victoria L.

doi:10.1021/ac00014a011

Cited by 12 publications

(8 citation statements)

References 26 publications

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“…, etc.) that cause the unevenly distributed mass pattern [5][6][7]. However, these explanations are of little consequence to practical users, as the instrumental conditions vary from one laboratory to another.…”

Section: Peak Asymmetrymentioning

confidence: 99%

Temporally convoluted Gaussian equations for chromatographic peaks

Pai

2004

Journal of Chromatography A

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Section: Peak Asymmetrymentioning

confidence: 99%

Temporally convoluted Gaussian equations for chromatographic peaks

Pai

2004

Journal of Chromatography A

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“…Detection System. Laser-induced fluorescence is utilized to probe solute zones at two to six positions along the microcolumn (3)(4)(5)(6)(7)(8)(9)(10). A continuous-wave He-Cd laser (325 nm, 10 mW) is utilized as the excitation source and is transmitted to the column via UVgrade optical fibers.…”

Section: On-column Laser-induced Fluorescence Detection Systemmentioning

confidence: 99%

“…Measured ratio of the length variance measured off-and on-column after injection as a function of the capacity factor. (--) Theoretical prediction according to Equation [1]; ( © ) Experimental measurement using a homologous series of fatty acids as model solutes (5).…”

Section: Figurementioning

confidence: 99%

“…Based on the results presented above, the interrelationship between the effects of temperature and pressure on solute retention may now be elucidated. By differentiation of Equation [3], the variation in capacity factor with temperature at constant pressure is expressed as ld(In k)] AH dT p -R T2 [5] From the experimental data shown in Figure 6, the values of d(In k)/dT decrease systematically from -0.012 to-0.029°C-1for solutes n-Clo to n-C2o,respectively, when evaluated at 1700 psi.…”

Section: Combined Effect Of Temperature and Pressurementioning

confidence: 99%

“…From our previous studies, we have gained experience in the preparation and characterization of uniformly packed beds of spherical particles. We have acquired a clear understanding of the retention and dispersion processes that occur as solute zones enter (5) and exit (6,7) the packed bed. Finally, we have elucidated the retention behavior of solute zones as a function of local temperature and pressure along the packed bed (8)(9)(10).…”

Section: /?mentioning

confidence: 99%

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Direct probing of chromatography columns by laser-induced fluorescence

McGuffin¹

1992

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This report summarizes the progress and accomplishments of this research project from September 1, 1989 to February 28, 1993. During this period, we have accomplished ali of the primary scientific objectives of the research proposal: 1) constructed and evaluated a laser-induced fluorescence detection system that allows direct examination of the chromatographic column, 2) examined nonequilibrium processes that occur upon solute injection and elution, 3) examined solute retention in liquid chromatography as a function of temperature and pressure, 4) examined solute zone dispersion in liquid chromatography as a function of temperature and pressure, and 5) developed appropriate theoretical models to describe these phenomena. In each of these studies, substantial knowledge has been gained of the fundamental processes that are responsible for chromatographic separations. lr, addition to these primary research objectives, we have made significant progress in three related areas: 1) examined pyrene as a fluorescent polarity probe in supercritical fluids and liquids as a function of temperature and pressure, 2) developed methods for the class-selective identification of polynuclear aromatic hydrocarbons in coal-derived fluids by microcolumn liquid chromatography with fluorescence quenching detection, and 3) developed methods for the determination of saturated and unsaturated (including omega-3) fatty acids in fish oil extracts by microcolumn liquid chromatography with laser-induced fluorescence detection. In these studies, the advanced separation and detection techniques developed in our laboratory are applied to practical problems of environmental and biomedical significance.

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Evaluation of a spatial scanning laser fluorometric detector for capillary electrophoresis: Application to studies of band migration and dispersion

Clark

1995

J Microcolumn Separations

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Abstract. We report herein the development and evaluation of modified capillary electrophoresis instrumentation with laser fluorometric detection which allows for the convenient and precise translation of the detection zone along the length of the separation column. This instrumentation is utilized for fundamental studies of band dispersion, analyte mobility alterations, sample stacking, and the determination of diffusion coefficients. The feasibility of using this instrumentation to improve the signal/noise ratio and decrease analysis time is also presented. DNA restriction digest separations employing soluble polymer buffer systems with oncolumn fluorescent labeling was used to illustrate the unique advantages of this experimental design. 0 1995 John Wiley & Sons, Inc.

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Direct examination of the injection process in liquid chromatographic separations

Cited by 12 publications

References 26 publications

Temporally convoluted Gaussian equations for chromatographic peaks

Temporally convoluted Gaussian equations for chromatographic peaks

Direct probing of chromatography columns by laser-induced fluorescence

Evaluation of a spatial scanning laser fluorometric detector for capillary electrophoresis: Application to studies of band migration and dispersion

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