Direct formation of mixed micelles in the solubilization of phospholipid liposomes by Triton X‐100

López, Olga; Maza, Alfons de la; Coderch, L.; López‐Iglesias, Carmen; Wehrli, E.; Parra, J. L.

doi:10.1016/s0014-5793(98)00363-9

Cited by 162 publications

(134 citation statements)

References 18 publications

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“…According to Lopez et al the entrapment of drug occurs both in the bilayer and in the aqueous compartment of the vesicle, for which both surfactant and lipids are responsible. 28 When the lipid compartment and the aqueous phase become saturated with the drug, the vesicle provides limited entrapment capacity. 29 SDC, with a steroidal structure and two OH groups, was used as a surfactant in this study.…”

Section: Mathematical Modelling For Rsm Optimizationmentioning

confidence: 99%

Experimental design and optimization of raloxifene hydrochloride loaded nanotransfersomes for transdermal application

Mahmood¹,

Taher²,

Mandal³

2014

IJN

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Raloxifene hydrochloride, a highly effective drug for the treatment of invasive breast cancer and osteoporosis in post-menopausal women, shows poor oral bioavailability of 2%. The aim of this study was to develop, statistically optimize, and characterize raloxifene hydrochloride-loaded transfersomes for transdermal delivery, in order to overcome the poor bioavailability issue with the drug. A response surface methodology experimental design was applied for the optimization of transfersomes, using Box-Behnken experimental design. Phospholipon ® 90G, sodium deoxycholate, and sonication time, each at three levels, were selected as independent variables, while entrapment efficiency, vesicle size, and transdermal flux were identified as dependent variables. The formulation was characterized by surface morphology and shape, particle size, and zeta potential. Ex vivo transdermal flux was determined using a Hanson diffusion cell assembly, with rat skin as a barrier medium. Transfersomes from the optimized formulation were found to have spherical, unilamellar structures, with a homogeneous distribution and low polydispersity index (0.08). They had a particle size of 134±9 nM, with an entrapment efficiency of 91.00%±4.90%, and transdermal flux of 6.5±1.1 μg/cm 2 /hour. Raloxifene hydrochloride-loaded transfersomes proved significantly superior in terms of amount of drug permeated and deposited in the skin, with enhancement ratios of 6.25±1.50 and 9.25±2.40, respectively, when compared with drug-loaded conventional liposomes, and an ethanolic phosphate buffer saline. Differential scanning calorimetry study revealed a greater change in skin structure, compared with a control sample, during the ex vivo drug diffusion study. Further, confocal laser scanning microscopy proved an enhanced permeation of coumarin-6-loaded transfersomes, to a depth of approximately160 μM, as compared with rigid liposomes. These ex vivo findings proved that a raloxifene hydrochloride-loaded transfersome formulation could be a superior alternative to oral delivery of the drug.

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Section: Mathematical Modelling For Rsm Optimizationmentioning

confidence: 99%

Experimental design and optimization of raloxifene hydrochloride loaded nanotransfersomes for transdermal application

Mahmood¹,

Taher²,

Mandal³

2014

IJN

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“…It is well known experimentally that detergents can dissolve lipid membranes into small micelles. 13,[18][19][20][21] Nomura et al 21 observed several types of dynamical behaviors in the lysis of liposomes: opening up by pore formation, inside-out inversion, burst, and vesicle shrinkage with oscillations. Pore-opening and rupture of vesicles were also observed under positive surface tension induced by adhesion to a surface, 22 optical illumination, 22,23 or micropipette aspiration.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of vesicle self-assembly and dissolution

Noguchi

Gompper

2006

The Journal of Chemical Physics

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The dynamics of membranes is studied on the basis of a particle-based meshless surface model, which was introduced earlier ͓Phys. Rev. E 73, 021903 ͑2006͔͒. The model describes fluid membranes with bending energy and-in the case of membranes with boundaries-line tension. The effects of hydrodynamic interactions are investigated by comparing Brownian dynamics with a particle-based mesoscale solvent simulation ͑multiparticle collision dynamics͒. Particles self-assemble into vesicles via disk-shaped membrane patches. The time evolution of assembly is found to consist of three steps: particle assembly into discoidal clusters, aggregation of clusters into larger membrane patches, and finally vesicle formation. The time dependence of the cluster distribution and the mean cluster size is evaluated and compared with the predictions of Smoluchowski rate equations. On the other hand, when the line tension is suddenly decreased ͑or the temperature is increased͒, vesicles dissolve via pore formation in the membrane. Hydrodynamic interactions are found to speed up the dynamics in both cases. Furthermore, hydrodynamics makes vesicle more spherical in the membrane-closure process.

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“…In particular, the sugar-type and the oxyethylene-type nonionic surfactants have been widely used for this purpose, and thus the nonionic surfactant-induced transition from phospholipid vesicles to micelles has been well studied [2][3][4][5][6][7][8][9][10][11]. Although studies using anionic [12][13][14][15][16][17], cationic [12,18], and zwitterionic surfactants [19] have also been published, they have received less attention.…”

Section: Introductionmentioning

confidence: 99%

Unique incorporation behavior of amino acid-type surfactant into phospholipid vesicle membrane

et al. 2005

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The incorporation behavior of some anionic surfactants, including amino acid-type surfactants, on phospholipid vesicles was investigated. This was done by measuring the release of a vesicle-entrapped fluorescence probe and the scattered light intensities of vesicle particles in the surfactant solution as a function of surfactant concentration and time. Sodium dodecyl sulfate, sodium dodecanesulfonate, sodium dodecanoyl sarcosinate, and sodium dodecanoyl glutaminate were employed in this study. All surfactants ruptured the phospholipid vesicle at around each critical micelle concentration by mixed micelle formation with phospholipid. While leakage of the fluorescence probe took place at a very low concentration in the sulfate-or sulfonate-type surfactant systems, it occurred at the concentration just below the CMC in the amino acid-type surfactant systems. Kinetic analysis of the release of the probe from the vesicles showed that the former surfactants adsorbed independently and homogeneously onto the phospholipid vesicles, while the latter surfactants were cooperatively incorporated.

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Direct formation of mixed micelles in the solubilization of phospholipid liposomes by Triton X‐100

Cited by 162 publications

References 18 publications

Experimental design and optimization of raloxifene hydrochloride loaded nanotransfersomes for transdermal application

Experimental design and optimization of raloxifene hydrochloride loaded nanotransfersomes for transdermal application

Dynamics of vesicle self-assembly and dissolution

Unique incorporation behavior of amino acid-type surfactant into phospholipid vesicle membrane

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