Direct gene transferState of the Art and Future Potential

Potrykus, I.; Shillito, Raymond D.; Saul, Michael W.; Paszkowski, Jerzy

doi:10.1007/bf02885591

Cited by 93 publications

(22 citation statements)

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“…The development and improvement of protoplast transformation methods with plasmid DNA by polyethylene glycol (PEG; Krens et al, 1982;Potrykus et al, 1985; Negrutiu et al, 1987), electroporation (Fromm et al, 1985; Nishiguchi et al, 1986; Ou-Lee et al., 1986; Hauptmann et al, 1987; Jones et al, 1989), and microinjection (Hillmer et al, 1992) set the foundation to use protoplasts to study gene regulation and signal transduction in plant cells. The establishment of new, and more economical, convenient and sensitive reporter gene assays for ␤-glucuronidase (GUS; Jefferson et al, 1987), chloramphenicol acyltransferase (Seed and Sheen, 1988), LUC (firefly luciferase; Luehrsen et al, 1992) and later green fluorescent protein (GFP;Sheen et al, 1995; Chiu et al, 1996) for plant cells has also facilitated the application of protoplast transient expression systems.…”

Section: A Brief History Of Protoplast Transient Expression Systemsmentioning

confidence: 99%

“…In particular, patch clamping of protoplasts is routinely used to study ion channels and their regulation by light, stress, or hormones (Moran et al, 1984;Schroeder et al, 1984Schroeder et al, , 2001 Maathuis and Sanders, 1994; Cho and Spalding, 1996; Bauer et al, 2000; Downey et al, 2000; Hamilton et al, 2000). Furthermore, protoplasts are frequently used to analyze calcium signals and regulation in plant cells (Gilroy and Jones, 1992; Trewavas, 1999; Pauly et al, 2000).The development and improvement of protoplast transformation methods with plasmid DNA by polyethylene glycol (PEG; Krens et al, 1982;Potrykus et al, 1985; Negrutiu et al, 1987), electroporation (Fromm et al, 1985; Nishiguchi et al, 1986; Ou-Lee et al., 1986; Hauptmann et al, 1987; Jones et al, 1989), and microinjection (Hillmer et al, 1992) set the foundation to use protoplasts to study gene regulation and signal transduction in plant cells. The establishment of new, and more economical, convenient and sensitive reporter gene assays for ␤-glucuronidase (GUS; Jefferson et al, 1987), chloramphenicol acyltransferase (Seed and Sheen, 1988), LUC (firefly luciferase; Luehrsen et al, 1992) and later green fluorescent protein (GFP;Sheen et al, 1995; Chiu et al, 1996) for plant cells has also facilitated the application of protoplast transient expression systems.…”

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Signal Transduction in Maize and Arabidopsis Mesophyll Protoplasts

2001

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Plant protoplasts show physiological perceptions and responses to hormones, metabolites, environmental cues, and pathogen-derived elicitors, similar to cell-autonomous responses in intact tissues and plants. The development of defined protoplast transient expression systems for high-throughput screening and systematic characterization of gene functions has greatly contributed to elucidating plant signal transduction pathways, in combination with genetic, genomic, and transgenic approaches.The availability of mutants, transgenic plants, global gene expression profiles, and genomic sequences has offered invaluable opportunities in understanding organismal plant biology at the cellular and molecular level (Gai et al., 2000; Genome, 2000; Parinov and Sundaresan, 2000;Richmond and Somerville, 2000;Sussman et al., 2000;Walbot, 2000;Zhu and Wang, 2000). Notably, molecular and genetic studies have discovered central components from receptors to transcription factors in diverse plant signal transduction pathways (Bleecker and Kende, 2000; Gray and Estelle, 2000; McCarty and Chory, 2000;Urao et al., 2000; Dangl and Jones, 2001; Inoue et al., 2001;Schroeder et al., 2001; Tena et al., 2001;Zhu, 2001). Still, many missing links exist in the plant transduction pathways from signals to target genes.Analogous to the mammalian tissue culture lines and transient gene expression assays that are indispensable for the rapid progress in discoveries of signal transduction pathways in multicellular organisms, protoplast transient expression systems using parsley (Petroselinum crispum), maize (Zea mays), carrot (Daucus carota), alfalfa (Medicago sativa), Arabidopsis, and tobacco (Nicotiana tabacum) suspension culture cells have been established. These plant cell lines offer new opportunities to dissect signal transduction pathways involved in UV (Lipphardt et al., 1988), abscisic acid (ABA; Vasil et al., 1989), metabolite (Loake et al., 1991), ribosomal RNA (Doelling and Pikaard, 1993), light (Frohnmeyer et al., 1994; Harter et al., 1994), auxin (Liu et al., 1994), defense (Nurnberger et al., 1994), and cell cycle regulation (Evans and Bravo, 1983; Nagata et al., 1992; Ito et al., 2001). Compared with cell culture lines, the use of fresh tissues as protoplast sources offers unique advantages. For example, protoplasts isolated from plant tissues retain their cell identity and differentiated state; they show high transformation efficiency with low maintenance. These freshly isolated protoplasts have proven to be physiological and versatile cell systems for studying a broad spectrum of plant signaling mechanisms underlying phytochrome, clock (Kim et al., 1993), auxin (Abel and Theologis, 1996), gibberellin (GA; Gubler et al., 1999), light, sugar, stress, auxin, hydrogen peroxide (Sheen, 1999; Tena et al., 2001), membrane transport (Maathuis et al., 1997; Bauer et al., 2000; Hamilton et al., 2000;Schroeder et al., 2001), ABA (Uno et al., 2000), cytokinin (Hwang and Sheen, 2001), and cell death (Asai et al., 2000; Bethke and Jone...

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Section: A Brief History Of Protoplast Transient Expression Systemsmentioning

confidence: 99%

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confidence: 99%

Signal Transduction in Maize and Arabidopsis Mesophyll Protoplasts

2001

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“…The process of direct gene transfer involves the integration of foreign genes into plant genomes following uptake of DNA into isolated protoplasts (Paszkowski et al ., 1984;Potrykus et al ., 1985 ;Negrutiu et al ., 1987), microinjecti bn of DNA into isolated protoplasts, protoplast-derived cells, or tissues (Crossway et al, 1986) or microprojectile bombardment of tissues (Klein et al ., 1987) . Since stable transformation with Agrobacterium strains harbouring genetically engineered Ti or Ri plasmids has been successful for several legumes, comparatively little work has been reported on the use of direct gene transfer for genetically engineering plants .…”

Section: Direct Gene Transfer Into Legumesmentioning

confidence: 99%

Genetic improvement of legumes using somatic cell and molecular techniques

Kumar

Davey

1991

Euphytica

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“…In contrast to the zygotic (sexual) embryos of seeds, somatic embryos arise from somatic cells (Figs. [1][2][3]. Somatic embryos can be cultivated in any desired number in synthetic media.…”

Section: Alternatively Somatic Embryos May Develop (R) a Cell Wall mentioning

confidence: 99%

Genetic Engineering with Plants

Eckes

Donn

Wengenmayer

1987

Angew. Chem. Int. Ed. Engl.

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Dedicated to Professor Heinz Harnisch on the occasion of his 60th birthdayToday there are no longer any basic problems involved in isolating and characterizing plant genes. Our knowledge of the structure and function of the genes so far sequenced provides a beginning understanding of gene regulation in plants. In connection with the recently developed methods of gene transfer to plant cells, the most important application is the production of plants with new characteristics through genetic engineering. Since the genes can be transferred from any kind of plant-indeed even from any organism-completely new possibilities are opened up for plant breeding.

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Direct gene transferState of the Art and Future Potential

Cited by 93 publications

References 22 publications

Signal Transduction in Maize and Arabidopsis Mesophyll Protoplasts

Signal Transduction in Maize and Arabidopsis Mesophyll Protoplasts

Genetic improvement of legumes using somatic cell and molecular techniques

Genetic Engineering with Plants

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